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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06380 [old locus tag: NWMN_1130 ]
  • pan locus tag?: SAUPAN003503000
  • symbol: NWMN_RS06380
  • pan gene symbol?: pknB
  • synonym:
  • product: serine/threonine protein kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS06380 [old locus tag: NWMN_1130 ]
  • symbol: NWMN_RS06380
  • product: serine/threonine protein kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1238160..1240154
  • length: 1995
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1238160..1240154) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
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    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGC
    ATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCG
    ATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACAT
    AACTCATCACAGCTATCACATCAAAATATAGTAAGTATGATCGATGTTGATGAAGAAGAT
    GACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAA
    AGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGC
    ATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTA
    ATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAG
    ACGTCTTTAACTCAGACTAATCATGTGTTAGGTACTGTGCAGTACTTTTCGCCAGAACAA
    GCAAAAGGTGAGGCAACGGATGAATGTACAGATATTTATTCTATAGGTATTGTGTTATAT
    GAAATGCTTGTTGGTGAACCACCCTTTAATGGAGAAACTGCAGTTAGCATTGCGATTAAA
    CATATTCAGGATTCTGTGCCAAATGTGACAACAGATGTACGTAAGGATATTCCGCAATCT
    TTAAGTAATGTCATTTTACGCGCTACAGAAAAAGACAAAGCGAATCGTTACAAAACAATT
    CAAGAAATGAAAGATGATTTGAGTAGTGTTTTACATGAAAATCGAGCGAATGAAGATGTC
    TATGAACTCGATAAAATGAAAACGATAGCGGTACCTTTGAAAAAAGAAGATCTAGCAAAG
    CATATTAGTGAACATAAGTCGAATCAACCTAAACGTGAAACGACGCAAGTACCTATTGTA
    AATGGGCCTGCTCATCATCAGCAATTCCAAAAGCCAGAAGGTACGGTGTACGAACCAAAA
    CCTAAAAAGAAATCAACACGAAAGATTGTGCTCTTATCACTAATCTTTTCGTTGTTAATG
    ATTGCACTTGTTTCTTTTGTGGCAATGGCAATGTTTGGTAATAAATACGAAGAGACACCT
    GATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAA
    TTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACA
    ACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAG
    GGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAG
    AAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCG
    CCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGAT
    TCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTATGTAGAAGACTTTGAA
    CATAAATCCTTTAGCAAAGCTAAAAAAGCCTTAGAAGAAAAAGGGTTTAAAGTTGAAAGT
    AAGGAAGAGTATAGTGACGATATTGATGAGGGTGATGTGATTTCTCAATCTCCTAAAGGA
    AAATCAGTAGATGAGGGGTCAACGATTTCATTTGTTGTTTCTAAAGGTAAAAAAAGTGAC
    TCATCAGATGTCAAAACGACAACTGAATCGGTAGATGTACCATACACTGGTAAAAATGAT
    AAGTCACAAAAAGTTAAAGTTTATATTAAAGATAAAGATAATGACGGTTCAACTGAAAAA
    GGTAGTTTCGATATTACTAGTGATCAACGTATAGACATTCCTTTAAGAATTGAAAAAGGA
    AAAACAGCAAGTTATATTGTTAAAGTTGACGGTAAAACTGTAGCTGAAAAAGAAGTCAGC
    TATGATGATGTATAA
    60
    120
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    1560
    1620
    1680
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    1860
    1920
    1980
    1995

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS06380 [old locus tag: NWMN_1130 ]
  • symbol: NWMN_RS06380
  • description: serine/threonine protein kinase
  • length: 664
  • theoretical pI: 5.9417
  • theoretical MW: 74362.8
  • GRAVY: -0.546837

Function[edit source | edit]

  • reaction:
    EC 2.7.11.1?  ExPASy
    Non-specific serine/threonine protein kinaseATP + a protein = ADP + a phosphoprotein
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistanceTOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 150.6)
    Unknown functionGeneralKae1-associated kinase Bud32 (TIGR03724; EC 2.7.-.-; HMM-score: 41.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-polyprenylphenol 6-hydroxylase (TIGR01982; EC 1.14.13.-; HMM-score: 16.9)
    early transcribed membrane protein (ETRAMP) family (TIGR01495; HMM-score: 14.5)
  • TheSEED:
  • PFAM:
    PKinase (CL0016) Pkinase; Protein kinase domain (PF00069; HMM-score: 196.3)
    no clan definedPASTA; PASTA domain (PF03793; HMM-score: 145.1)
    PKinase (CL0016) Pkinase_Tyr; Protein tyrosine kinase (PF07714; HMM-score: 134.6)
    APH; Phosphotransferase enzyme family (PF01636; HMM-score: 27)
    Seadorna_VP7; Seadornavirus VP7 (PF07387; HMM-score: 15.7)
    RIO1; RIO1 family (PF01163; HMM-score: 15.2)
    no clan definedSigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 15.2)
    ChlamPMP_M; Chlamydia polymorphic membrane protein middle domain (PF07548; HMM-score: 14.9)
    PKinase (CL0016) Choline_kinase; Choline/ethanolamine kinase (PF01633; HMM-score: 14.7)
    Kinase-like; Kinase-like (PF14531; HMM-score: 13.8)
    no clan definedETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 13.4)
    PKinase (CL0016) Kdo; Lipopolysaccharide kinase (Kdo/WaaP) family (PF06293; HMM-score: 12.8)
    no clan definedPNPase; Polyribonucleotide nucleotidyltransferase, RNA binding domain (PF03726; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 11
    • Cmax: 0.131
    • Cmax_pos: 27
    • Smax: 0.174
    • Smax_pos: 36
    • Smean: 0.133
    • D: 0.123
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

  • GI: 446501710 NCBI
  • RefSeq: WP_000579564 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIPPREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESHGPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSETSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIAIKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRANEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEGTVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQIFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNVIGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYESLGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEGSTISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFDITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS06380 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]