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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01187
  • pan locus tag?: SAUPAN003503000
  • symbol: SAOUHSC_01187
  • pan gene symbol?: pknB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01187
  • symbol: SAOUHSC_01187
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1138203..1140197
  • length: 1995
  • essential: no [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGC
    ATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCG
    ATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACAT
    AACTCATCACAGCTATCACATCAAAATATAGTAAGTATGATCGATGTTGATGAAGAAGAT
    GACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAA
    AGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGC
    ATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTA
    ATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAG
    ACGTCTTTAACTCAGACTAATCATGTGTTAGGTACTGTGCAGTACTTTTCGCCAGAACAA
    GCAAAAGGTGAGGCAACGGATGAATGTACAGATATTTATTCTATAGGTATTGTGTTATAT
    GAAATGCTTGTTGGTGAACCACCCTTTAATGGAGAAACTGCAGTTAGCATTGCGATTAAA
    CATATTCAGGATTCTGTGCCAAATGTGACAACAGATGTACGTAAGGATATTCCGCAATCT
    TTAAGTAATGTCATTTTACGCGCTACAGAAAAAGACAAAGCGAATCGTTACAAAACAATT
    CAAGAAATGAAAGATGATTTGAGTAGTGTTTTACATGAAAATCGAGCGAATGAAGATGTC
    TATGAACTCGATAAAATGAAAACGATAGCGGTACCTTTGAAAAAAGAAGATCTAGCAAAG
    CATATTAGTGAACATAAGTCGAATCAACCTAAACGTGAAACGACGCAAGTACCTATTGTA
    AATGGGCCTGCTCATCATCAGCAATTCCAAAAGCCAGAAGGTACGGTGTACGAACCAAAA
    CCTAAAAAGAAATCAACACGAAAGATTGTGCTCTTATCACTAATCTTTTCGTTGTTAATG
    ATTGCACTTGTTTCTTTTGTGGCAATGGCAATGTTTGGTAATAAATACGAAGAGACACCT
    GATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAA
    TTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACA
    ACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAG
    GGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGCCTTGCAG
    AAATTAAAATCGTTAGGTCTTAAAGATGTTACGATTGAAAAAGTATATAATAATCAAGCG
    CCAAAAGGATACATTGCAAATCAAAGTGTAACCGCAAATACTGAAATCGCTATTCATGAT
    TCTAATATTAAACTATATGAATCTTTAGGCATTAAGCAAGTTTATGTAGAAGACTTTGAA
    CATAAATCCTTTAGCAAAGCTAAAAAAGCCTTAGAAGAAAAAGGGTTTAAAGTTGAAAGT
    AAGGAAGAGTATAGTGACGATATTGATGAGGGTGATGTGATTTCTCAATCTCCTAAAGGA
    AAATCAGTAGATGAGGGGTCAACGATTTCATTTGTTGTTTCTAAAGGTAAAAAAAGTGAC
    TCATCAGATGTCAAAACGACAACTGAATCGGTAGATGTACCATACACTGGTAAAAATGAT
    AAGTCACAAAAAGTTAAAGTTTATATTAAAGATAAAGATAATGACGGTTCAACTGAAAAA
    GGTAGTTTCGATATTACTAGTGATCAACGTATAGACATTCCTTTAAGAATTGAAAAAGGA
    AAAACAGCAAGTTATATTGTTAAAGTTGACGGTAAAACTGTAGCTGAAAAAGAAGTCAGC
    TATGATGATGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1995

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01187
  • symbol: SAOUHSC_01187
  • description: hypothetical protein
  • length: 664
  • theoretical pI: 5.9417
  • theoretical MW: 74362.8
  • GRAVY: -0.546837

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistanceTOMM system kinase/cyclase fusion protein (TIGR03903; HMM-score: 150.6)
    Unknown functionGeneralKae1-associated kinase Bud32 (TIGR03724; EC 2.7.-.-; HMM-score: 41.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-polyprenylphenol 6-hydroxylase (TIGR01982; EC 1.14.13.-; HMM-score: 16.9)
    early transcribed membrane protein (ETRAMP) family (TIGR01495; HMM-score: 14.5)
  • TheSEED:  
    A Gram-positive cluster that relates ribosomal protein L28P to a set of uncharacterized proteins Serine/threonine protein kinase PrkC, regulator of stationary phase 
    Conserved gene cluster associated with Met-tRNA formyltransferase Serine/threonine protein kinase PrkC, regulator of stationary phase 
  • PFAM:
    PKinase (CL0016) Pkinase; Protein kinase domain (PF00069; HMM-score: 196.3)
    no clan definedPASTA; PASTA domain (PF03793; HMM-score: 145.1)
    PKinase (CL0016) Pkinase_Tyr; Protein tyrosine kinase (PF07714; HMM-score: 134.6)
    APH; Phosphotransferase enzyme family (PF01636; HMM-score: 27)
    Seadorna_VP7; Seadornavirus VP7 (PF07387; HMM-score: 15.7)
    RIO1; RIO1 family (PF01163; HMM-score: 15.2)
    no clan definedSigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 15.2)
    ChlamPMP_M; Chlamydia polymorphic membrane protein middle domain (PF07548; HMM-score: 14.9)
    PKinase (CL0016) Choline_kinase; Choline/ethanolamine kinase (PF01633; HMM-score: 14.7)
    Kinase-like; Kinase-like (PF14531; HMM-score: 13.8)
    no clan definedETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 13.4)
    PKinase (CL0016) Kdo; Lipopolysaccharide kinase (Kdo/WaaP) family (PF06293; HMM-score: 12.8)
    no clan definedPNPase; Polyribonucleotide nucleotidyltransferase, RNA binding domain (PF03726; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 11
    • Cmax: 0.131
    • Cmax_pos: 27
    • Smax: 0.174
    • Smax_pos: 36
    • Smean: 0.133
    • D: 0.123
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIPPREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESHGPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSETSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIAIKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRANEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEGTVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQIFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNVIGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYESLGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEGSTISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFDITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Stefanie Donat, Karin Streker, Tanja Schirmeister, Sonja Rakette, Thilo Stehle, Manuel Liebeke, Michael Lalk, Knut Ohlsen
Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus.
J. Bacteriol.: 2009, 191(13);4056-69
[PubMed:19376851] [WorldCat.org] [DOI] (I p)
Malgorzata Miller, Stefanie Donat, Sonja Rakette, Thilo Stehle, Thijs R H M Kouwen, Sander H Diks, Annette Dreisbach, Ewoud Reilman, Katrin Gronau, Dörte Becher, Maikel P Peppelenbosch, Jan Maarten van Dijl, Knut Ohlsen
Staphylococcal PknB as the first prokaryotic representative of the proline-directed kinases.
PLoS ONE: 2010, 5(2);e9057
[PubMed:20140229] [WorldCat.org] [DOI] (I e)