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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01185
  • pan locus tag?: SAUPAN003501000
  • symbol: SAOUHSC_01185
  • pan gene symbol?: rlmN
  • synonym:
  • product: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01185
  • symbol: SAOUHSC_01185
  • product: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
  • replicon: chromosome
  • strand: +
  • coordinates: 1136362..1137456
  • length: 1095
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGATAACTGCTGAAAAGAAAAAGAAGAATAAATTTCTTCCAAATTTTGACAAGCAATCA
    ATATATTCATTGCGATTTGACGAAATGCAAAACTGGCTCGTTGAACAAGGGCAACAAAAA
    TTTCGAGCGAAACAGATTTTTGAATGGTTATATCAAAAAAGAGTAGATTCGATTGATGAA
    ATGACGAACTTATCGAAAGACTTACGACAGCTTTTAAAAGATAACTTTACTGTTACAACT
    TTAACAACTGTAGTAAAACAAGAAAGTAAAGACGGTACAATTAAATTCTTATTTGAATTA
    CAAGATGGCTATACAATTGAAACTGTTTTAATGAGACATGATTATGGAAATTCAGTATGT
    GTAACGACACAAGTAGGTTGTCGCATCGGATGTACGTTTTGTGCTTCTACACTTGGCGGC
    TTAAAAAGAAACCTTGAAGCTGGCGAAATTGTTTCACAAGTTTTAACAGTTCAAAAAGCC
    CTTGATGCTACAGAAGAGCGCGTATCTCAAATTGTCATAATGGGTATCGGTGAACCATTT
    GAAAATTATGATGAAATGATGGACTTTTTAAGAATCGTCAATGATGATAACAGTTTAAAT
    ATTGGTGCACGTCACATTACAGTATCAACATCAGGTATCATTCCTAGAATATATGACTTT
    GCGGATGAAGATATTCAAATTAATTTTGCTGTAAGCTTACACGCCGCAAAAGATGAAGTG
    CGATCACGCTTGATGCCAATTAACCGTGCATATAATGTTGAGAAGTTAATCGAAGCAATT
    CAATATTATCAAGAAAAAACAAATCGTCGTGTTACTTTTGAATATGGTCTGTTTGGTGGT
    GTGAATGACCAACTAGAACATGCAAGAGAATTAGCACATTTAATAAAAGGCTTAAACTGC
    CATGTTAACTTAATTCCTGTCAACCATGTTCCAGAAAGAAATTATGTGAAAACGGCTAAA
    AATGATATCTTTAAATTTGAAAAAGAATTAAAGAGACTAGGAATTAATGCCACAATACGT
    CGTGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAA
    GTAGAAACGAGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1095

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01185
  • symbol: SAOUHSC_01185
  • description: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
  • length: 364
  • theoretical pI: 7.15306
  • theoretical MW: 41904.6
  • GRAVY: -0.428022

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.192?  ExPASy
    23S rRNA (adenine2503-C2)-methyltransferase 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin 2 S-adenosyl-L-methionine + adenine37 in tRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA + 2 oxidized [2Fe-2S] ferredoxin
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(2))-methyltransferase (TIGR00048; EC 2.1.1.192; HMM-score: 510.6)
    and 9 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (adenine(2503)-C(8))-methyltransferase Cfr (TIGR04432; EC 2.1.1.224; HMM-score: 251.5)
    hopanoid biosynthesis associated radical SAM protein HpnH (TIGR03470; HMM-score: 22.6)
    Genetic information processing Protein fate Protein modification and repair anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 19.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 19.3)
    radical SAM enzyme, TIGR04100 family (TIGR04100; HMM-score: 19.1)
    Cellular processes Cellular processes Adaptations to atypical conditions KamA family protein (TIGR00238; HMM-score: 17.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02826; EC 1.97.1.-; HMM-score: 15.1)
    Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium transporter (TIGR00400; HMM-score: 10.5)
  • TheSEED  :
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • tRNA (adenine(37)-C(2))-methyltransferase (EC 2.1.1.192)
    Conserved gene cluster associated with Met-tRNA formyltransferase  Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 69.1)
    and 4 more
    4Fe-4S (CL0344) Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 29.7)
    Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 19.4)
    no clan defined Pox_RNA_Pol_22; Poxvirus RNA polymerase 22 kDa subunit (PF05273; HMM-score: 13.4)
    Peptidase_CA (CL0125) Peptidase_C23; Carlavirus endopeptidase (PF05379; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001615
    • TAT(Tat/SPI): 0.000149
    • LIPO(Sec/SPII): 0.000265
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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