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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01185
  • pan locus tag?: SAUPAN003501000
  • symbol: SAOUHSC_01185
  • pan gene symbol?: rlmN
  • synonym:
  • product: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01185
  • symbol: SAOUHSC_01185
  • product: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
  • replicon: chromosome
  • strand: +
  • coordinates: 1136362..1137456
  • length: 1095
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGATAACTGCTGAAAAGAAAAAGAAGAATAAATTTCTTCCAAATTTTGACAAGCAATCA
    ATATATTCATTGCGATTTGACGAAATGCAAAACTGGCTCGTTGAACAAGGGCAACAAAAA
    TTTCGAGCGAAACAGATTTTTGAATGGTTATATCAAAAAAGAGTAGATTCGATTGATGAA
    ATGACGAACTTATCGAAAGACTTACGACAGCTTTTAAAAGATAACTTTACTGTTACAACT
    TTAACAACTGTAGTAAAACAAGAAAGTAAAGACGGTACAATTAAATTCTTATTTGAATTA
    CAAGATGGCTATACAATTGAAACTGTTTTAATGAGACATGATTATGGAAATTCAGTATGT
    GTAACGACACAAGTAGGTTGTCGCATCGGATGTACGTTTTGTGCTTCTACACTTGGCGGC
    TTAAAAAGAAACCTTGAAGCTGGCGAAATTGTTTCACAAGTTTTAACAGTTCAAAAAGCC
    CTTGATGCTACAGAAGAGCGCGTATCTCAAATTGTCATAATGGGTATCGGTGAACCATTT
    GAAAATTATGATGAAATGATGGACTTTTTAAGAATCGTCAATGATGATAACAGTTTAAAT
    ATTGGTGCACGTCACATTACAGTATCAACATCAGGTATCATTCCTAGAATATATGACTTT
    GCGGATGAAGATATTCAAATTAATTTTGCTGTAAGCTTACACGCCGCAAAAGATGAAGTG
    CGATCACGCTTGATGCCAATTAACCGTGCATATAATGTTGAGAAGTTAATCGAAGCAATT
    CAATATTATCAAGAAAAAACAAATCGTCGTGTTACTTTTGAATATGGTCTGTTTGGTGGT
    GTGAATGACCAACTAGAACATGCAAGAGAATTAGCACATTTAATAAAAGGCTTAAACTGC
    CATGTTAACTTAATTCCTGTCAACCATGTTCCAGAAAGAAATTATGTGAAAACGGCTAAA
    AATGATATCTTTAAATTTGAAAAAGAATTAAAGAGACTAGGAATTAATGCCACAATACGT
    CGTGAACAAGGTTCGGATATTGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAA
    GTAGAAACGAGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1095

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01185
  • symbol: SAOUHSC_01185
  • description: 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN
  • length: 364
  • theoretical pI: 7.15306
  • theoretical MW: 41904.6
  • GRAVY: -0.428022

Function[edit source | edit]

  • reaction:
    EC 2.1.1.192?  ExPASy
    23S rRNA (adenine2503-C2)-methyltransferase2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin2 S-adenosyl-L-methionine + adenine37 in tRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA + 2 oxidized [2Fe-2S] ferredoxin
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modification23S rRNA (adenine(2503)-C(2))-methyltransferase (TIGR00048; EC 2.1.1.192; HMM-score: 510.6)
    Genetic information processingProtein synthesistRNA and rRNA base modification23S rRNA (adenine(2503)-C(8))-methyltransferase Cfr (TIGR04432; EC 2.1.1.224; HMM-score: 251.5)
    hopanoid biosynthesis associated radical SAM protein HpnH (TIGR03470; HMM-score: 22.6)
    Genetic information processingProtein fateProtein modification and repairanaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 19.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismanaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 19.3)
    radical SAM enzyme, TIGR04100 family (TIGR04100; HMM-score: 19.1)
    Cellular processesCellular processesAdaptations to atypical conditionsKamA family protein (TIGR00238; HMM-score: 17.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationMiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismanaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02826; EC 1.97.1.-; HMM-score: 15.1)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmagnesium transporter (TIGR00400; HMM-score: 10.5)
  • TheSEED:  
    Conserved gene cluster associated with Met-tRNA formyltransferase 23S rRNA (adenine(2503)-C(2))-methyltransferase 
    Conserved gene cluster associated with Met-tRNA formyltransferase tRNA (adenine(37)-C(2))-methyltransferase (EC 2.1.1.192) 
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 69.1)
    4Fe-4S (CL0344) Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 29.7)
    Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 19.4)
    no clan definedPox_RNA_Pol_22; Poxvirus RNA polymerase 22 kDa subunit (PF05273; HMM-score: 13.4)
    Peptidase_CA (CL0125) Peptidase_C23; Carlavirus endopeptidase (PF05379; HMM-score: 12.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 11
    • Cmax: 0.113
    • Cmax_pos: 38
    • Smax: 0.279
    • Smax_pos: 1
    • Smean: 0.18
    • D: 0.156
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]