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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09365 [old locus tag: NWMN_1668 ]
  • pan locus tag?: SAUPAN004470000
  • symbol: NWMN_RS09365
  • pan gene symbol?: sigS
  • synonym:
  • product: RNA polymerase sigma factor SigS

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS09365 [old locus tag: NWMN_1668 ]
  • symbol: NWMN_RS09365
  • product: RNA polymerase sigma factor SigS
  • replicon: chromosome
  • strand: +
  • coordinates: 1865174..1865644
  • length: 471
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1865174..1865644) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGAAATTTAATGACGTATACAACAAACACCACAAAATCATACACCATCTTTTAAAAAAA
    TATAATATTAGCTATAATTATGATGAGTATTATCAACTACTCTTGATAAAAATGTGGCAA
    TTGAGTCAGATATATAAACCCTCAAGCAAGCAATCTTTATCCTCTTTTTTATTCACTCGA
    TTAAATTTTTACCTTATCGATTTATTCAGACAACAAAATCAATTAAAAGATGTCATTTTA
    TGTGAGAATAATTCACCAACATTAACTGAACAACCAACATACTTTAATGAACATGACCTT
    CGTTTACAAGATATCTTCAAGCTTTTAAATCAAAGAGAAAGACTATGGCTCAAACTATAC
    CTTGAAGGATACAAGCAATTTGAAATTGCTGAAATCATGTCATTATCGCTTTCAACGATT
    AAATTAATTAAGATGTCCGTTAAGCGTAAATGCCAACATAATTTTAATTAG
    60
    120
    180
    240
    300
    360
    420
    471

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS09365 [old locus tag: NWMN_1668 ]
  • symbol: NWMN_RS09365
  • description: RNA polymerase sigma factor SigS
  • length: 156
  • theoretical pI: 9.80207
  • theoretical MW: 19136.1
  • GRAVY: -0.479487

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 61.5)
    transcriptional regulator BotR, P-21 (TIGR03209; HMM-score: 28.9)
    RNA polymerase sigma factor, SigZ family (TIGR02959; HMM-score: 25.7)
    RNA polymerase sigma factor, FliA/WhiG family (TIGR02479; HMM-score: 23.9)
    RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 22.6)
    RNA polymerase sigma-70 factor, TIGR02954 family (TIGR02954; HMM-score: 16.5)
    RNA polymerase sigma factor, TIGR02999 family (TIGR02999; HMM-score: 15.1)
    LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 15)
    Cellular processesCellular processesSporulation and germinationRNA polymerase sigma-G factor (TIGR02850; HMM-score: 12.4)
    Genetic information processingTranscriptionTranscription factorsRNA polymerase sigma-G factor (TIGR02850; HMM-score: 12.4)
    RNA polymerase sigma-B factor (TIGR02941; HMM-score: 11.9)
    RNA polymerase sigma-70 factor, sigma-E family (TIGR02983; HMM-score: 11.9)
  • TheSEED:
  • PFAM:
    HTH (CL0123) Sigma70_r2; Sigma-70 region 2 (PF04542; HMM-score: 28.9)
    GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 24.3)
    Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 20)
    Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 19.5)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.2)
    Terminase_5; Putative ATPase subunit of terminase (gpP-like) (PF06056; HMM-score: 12.8)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.1)
    no clan definedDUF3837; Domain of unknown function (DUF3837) (PF12939; HMM-score: 9.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 11
    • Cmax: 0.115
    • Cmax_pos: 51
    • Smax: 0.239
    • Smax_pos: 8
    • Smean: 0.177
    • D: 0.147
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446593706 NCBI
  • RefSeq: WP_000671052 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQIYKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRLQDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS09365 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]