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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0566 [new locus tag: SA_RS03275 ]
  • pan locus tag?: SAUPAN002476000
  • symbol: SA0566
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0566 [new locus tag: SA_RS03275 ]
  • symbol: SA0566
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 660074..660961
  • length: 888
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGAAGAAATCGTTAATTGCTTTTATTTTGATTTTTATGCTTGTCCTGAGTGGCTGTGGT
    ATGAAAGATAATGATAAACAAGGTAGCGATGATAATGGCTCGTCTAAATCGCCGTACCAT
    AGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAA
    TACATAGTTGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAGAGGGTAAGAAA
    CAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATT
    CTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTAGAGAAACAA
    GGCATCAAAGTAGTGTATGTTAAAGATGCACAATCAATTGATGAAACATACAACACATTT
    AAGCAAATTGGGAAATTAACGCATCATGATAAGCAGGCTGAACAACTTGTTGAGGAAACT
    AAAGATAATATCGATAAAGTCATAGATTCAATTCCTGCTCATCATAAAAAATCAAAAGTA
    TTTATTGAGGTTTCATCAAAGCCTGAAATATATACAGCAGGGAAGCATACATTTTTTAAT
    GATATGTTAGAAAAATTAGAAGCCCAAAATGTTTATAGTGACATTAATGGTTGGAACCCT
    GTAACGAAGGAAAGTATTATTAAAAAGAACCCAGATATATTAATTTCGACGGAAGCTAAG
    ACAAGATCAGATTATATGGATATCATCAAAAAAAGGGGTGGATTCAATAAAATTAATGCT
    GTCAAGAATACACGTATTGAAGTTGTAAATGGTGATGAAGTATCAAGACCAGGTCCACGT
    ATTGATGAAGGATTAAAAGAATTAAGAGATGCAATTTATAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    888

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0566 [new locus tag: SA_RS03275 ]
  • symbol: SA0566
  • description: hypothetical protein
  • length: 295
  • theoretical pI: 9.04982
  • theoretical MW: 33256.8
  • GRAVY: -0.572881

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 125.4)
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 75)
    proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 54.1)
  • TheSEED:  
    ABC transporter, substrate-binding protein (cluster 8, B12/iron complex) 
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 117.4)
    HTH (CL0123) F-112; F-112 protein (PF09645; HMM-score: 17.5)
    no clan definedMMR1; Mitochondrial Myo2 receptor-related protein (PF08505; HMM-score: 14.9)
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 13.7)
    Chelatase (CL0043) Oxidored_nitro; Nitrogenase component 1 type Oxidoreductase (PF00148; HMM-score: 13.6)
    no clan definedETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 13.5)
    SIS (CL0067) DUF2529; Domain of unknown function (DUF2529) (PF10740; HMM-score: 13.4)
    Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0.33
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LVLSGCG
  • SignalP: Signal peptide length 29 aa
    • Ymax: 0.339
    • Ymax_pos: 29
    • Cmax: 0.192
    • Cmax_pos: 29
    • Smax: 0.886
    • Smax_pos: 18
    • Smean: 0.663
    • D: 0.465
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVSLMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKAKPDLILAHESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEETKDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGWNPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSRPGPRIDEGLKELRDAIYRK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]