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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0850 [new locus tag: SA_RS04820 ]
  • pan locus tag?: SAUPAN003146000
  • symbol: SA0850
  • pan gene symbol?: opp-4A
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0850 [new locus tag: SA_RS04820 ]
  • symbol: SA0850
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 963927..965642
  • length: 1716
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGAAGCTAATTAAATATATTTCAATACTTCTTATTGTCGTTTTAGTGTTGAGTGCT
    TGCGGAAAAAGCAGTAATAAAGATGAAGGAGTAAAAGATGCTACTAAAACGGAAACCTCA
    AAACATAAAGGTGGTACCTTAAATGTAGCATTAACAGCACCGCCAAGTGGTGTTTATTCT
    TCGTTATTAAATAGTACACATGCAGATTCTGTAGTTGAGGGATATTTTAACGAAAGCTTA
    TTAGCAACTGATAAAAAAATACGTCCTAAGGCATATATTGCTTCATGGAAGGACATCGAG
    CCGGCTAAGAAAATAGAATTTAAAATTAAAAAAGGTATTAAATGGCATGATGGTAATGAA
    TTGAAAATTGATGATTGGATTTATTCAATTGAAGTCTTAGCTAACAAGGACTACGAAGGT
    GCTTATTATCCAAGTGTAGAAAATATCCAAGGTGCGAAAGATTATCATGAAGGAAAAACT
    GATCATATTAGCGGATTGAAGAAAATAGATGACTACACTATGCAGGTTACATTTGATAAA
    AAACAAGAAAATTACTTAACAGGATTTATTACTGGACATTTATTAAGTAAAAAATATTTA
    TCAGATGTACCAATTAAAGATTTAGCGAAATCAGATAAAATCCGAAAATATCCTATTGGT
    ATTGGACCGTATAAAGTTAAGAAAATCGTTCCAGGTGAGGCTGTTCAACTCGTTAAATTT
    GATGATTATTGGCAAGGTAAGCCTGCACTAGACAAAATCAATTTAAAAGTTATTGATCAA
    GCTCAAATTATTAAGGCAATGGAAAAAGGCGATATTGATGTTGCGAATGATGCTACCGGT
    GCAATGGCAAAAGATGCTAAGTCATCTAATGCTGGTCTCAAGGTATTATCTGCGCCAAGC
    TTAGACTACGGTTTAATAGGATTCGTATCTCATGATTACGATAAAAAAGCTAATAAAACT
    GGTAAAGTGAGACCAAAATATGAAGACAAAGAATTACGTAAAGCAATGCTTTATGCAATT
    GATAGAGAAAAATGGATTAAAGCGTTTTTCAATGGTTACGCTAGTGAAATCAATAGTTTT
    GTACCATCTATGCATTGGATAGCAGCCAATCCTAAGGACCTAAATGATTACAAATATGAT
    CCTGAAAAAGCTAAAAAAATCTTAGATAAGTTAGGTTATAAAGATAGAGATGGTGACGGA
    TTTAGAGAAGATCCTAAAGGTAATAAATTTGAGATTAACTTTAAACATTATTCAGGTTCA
    AATCCTACTTTTGAACCAAGAACTGCTGCGATAAAAGATTTCTGGGAAAAAGTTGGCTTG
    AAAACAAATGTGAAGTTAGTAGAATTCGGTAAATATAATGAAGACTTAGCAAATGCATCT
    AAAGATATGGAAGTGTACTTCAGATCATGGGCAGGAGGTACAGATCCAGATCCATCAGAT
    TTATACCACACTGATAGACCTCAAAATGAAATGAGAACAGTTTTACCAAAATCAGATCAA
    TATTTAGATGATGCATTAGACTTCGAAAAAGTAGGCATTGATGAAAAGAAACGTAAAGAT
    ATTTATGTTAAATGGCAAAAATATATGAATGATGAGTTACCTGGATTACCAATGTTCCAA
    GGTAAATCGATAACTATTGTTAACGATAAAGTACGAAACTTAGACATTGAAATTGGAACT
    GATCAAAGTTTATATAATTTAACTAAAGAAGCTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1716

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0850 [new locus tag: SA_RS04820 ]
  • symbol: SA0850
  • description: hypothetical protein
  • length: 571
  • theoretical pI: 9.00454
  • theoretical MW: 64616.1
  • GRAVY: -0.639405

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 94.2)
    MetabolismTransport and binding proteinsUnknown substrateABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 79.2)
  • TheSEED:  
    Membrane TransportABC transportersABC transporter oligopeptide (TC 3.A.1.5.1) Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) 
    Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) 
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 265.2)
    GBD (CL0202) P_proprotein; Proprotein convertase P-domain (PF01483; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LVLSACG
  • SignalP: Signal peptide length 28 aa
    • Ymax: 0.473
    • Ymax_pos: 28
    • Cmax: 0.273
    • Cmax_pos: 34
    • Smax: 0.926
    • Smax_pos: 19
    • Smean: 0.817
    • D: 0.607
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGKLIKYISILLIVVLVLSACGKSSNKDEGVKDATKTETSKHKGGTLNVALTAPPSGVYSSLLNSTHADSVVEGYFNESLLATDKKIRPKAYIASWKDIEPAKKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKTDHISGLKKIDDYTMQVTFDKKQENYLTGFITGHLLSKKYLSDVPIKDLAKSDKIRKYPIGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVANDATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKANKTGKVRPKYEDKELRKAMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAANPKDLNDYKYDPEKAKKILDKLGYKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFGKYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDFEKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYNLTKEA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]