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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1048 [new locus tag: SA_RS05955 ]
  • pan locus tag?: SAUPAN003485000
  • symbol: pyrE
  • pan gene symbol?: pyrE
  • synonym:
  • product: orotate phosphoribosyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1048 [new locus tag: SA_RS05955 ]
  • symbol: pyrE
  • product: orotate phosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1187106..1187717
  • length: 612
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAAT
    GATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACG
    TTAGGTTATCCTTTAGTTCGAGGCGCAATCCGCGATGGTTTAATTAACTTAATTAAAGAA
    CACTTTCCTGAAGTAGAAGTTATTTCTGGTACTGCAACAGCTGGTATTCCACATGCAGCT
    TTTATTGCTGAAAAATTAAAATTACCAATGAATTATGTTCGTTCATCAAATAAGAGTCAT
    GGTAAGCAAAATCAAATCGAAGGTGCTAAAAGTGAAGGTAAAAAAGTAGTTGTGATAGAA
    GATTTAATTTCGACAGGGGGATCTTCAGTCACAGCAGTTGAAGCCTTAAAACAAGCAGGT
    GCAGAAGTATTAGGTGTTGTAGCTATCTTTACTTACGGTTTGAAAAAAGCAGATGATACA
    TTTAGCAATATTCAACTACCTTTTTACACTTTAAGTGATTACAATGAATTAATTGAAGTA
    GCTGAAAATGAAGGTAAAATTTCTAGTGAAGATATCCAAACATTAGTTGAATGGAGAGAC
    AACTTAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    612

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1048 [new locus tag: SA_RS05955 ]
  • symbol: PyrE
  • description: orotate phosphoribosyltransferase
  • length: 203
  • theoretical pI: 4.79327
  • theoretical MW: 22056.9
  • GRAVY: -0.0684729

Function[edit | edit source]

  • reaction:
    EC 2.4.2.10?  ExPASy
    Orotate phosphoribosyltransferase Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 150.9)
    and 9 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 62.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 40)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 34.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides xanthine phosphoribosyltransferase (TIGR01744; EC 2.4.2.22; HMM-score: 24.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis pur operon repressor PurR (TIGR01743; HMM-score: 21.2)
    Signal transduction Regulatory functions DNA interactions pur operon repressor PurR (TIGR01743; HMM-score: 21.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uracil phosphoribosyltransferase (TIGR01091; EC 2.4.2.9; HMM-score: 18.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 17.4)
    Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 15.9)
  • TheSEED  :
    • Orotate phosphoribosyltransferase (EC 2.4.2.10)
    Nucleosides and Nucleotides Pyrimidines De Novo Pyrimidine Synthesis  Orotate phosphoribosyltransferase (EC 2.4.2.10)
  • PFAM:
    PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 67.1)
    and 3 more
    UPRTase; Uracil phosphoribosyltransferase (PF14681; HMM-score: 17.3)
    Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 13.9)
    Leu-IlvD (CL0364) Aconitase_2_N; Aconitate hydratase 2 N-terminus (PF06434; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008509
    • TAT(Tat/SPI): 0.000197
    • LIPO(Sec/SPII): 0.000517
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYPLVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGKQNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDTFSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: pyrR leader (transcription antitermination) regulon
    pyrR leader(RNA)important in Pyrimidine metabolism; RegPrecise binding of the PyrR antitermination protein

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)