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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1073 [new locus tag: SA_RS06080 ]
  • pan locus tag?: SAUPAN003514000
  • symbol: fabD
  • pan gene symbol?: fabD
  • synonym:
  • product: malonyl CoA-ACP transacylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1073 [new locus tag: SA_RS06080 ]
  • symbol: fabD
  • product: malonyl CoA-ACP transacylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1213830..1214756
  • length: 927
  • essential: yes DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTAAAACAGCAATTATTTTTCCGGGACAAGGTGCCCAAAAAGTTGGTATGGCACAA
    GATTTGTTTAATAACAATGATCAAGCAACTGAAATTTTAACTTCAGCAGCAAAGACGTTA
    GACTTTGATATTTTAGAGACAATGTTTACTGATGAAGAAGGTAAATTGGGTGAAACTGAA
    AACACGCAACCAGCTTTATTGACGCATAGTTCGGCATTATTAGCAGCGCTAAAAAATTTG
    AATCCTGATTTTACTATGGGGCATAGTTTAGGTGAATATTCAAGTTTAGTTGCAGCTGAC
    GTATTATCATTTGAAGATGCAGTTAAAATTGTTAGAAAACGTGGTCAATTAATGGCGCAA
    GCATTTCCTACTGGTGTAGGAAGCATGGCTGCAGTATTGGGATTAGATTTTGATAAAGTC
    GATGAAATTTGTAAGTCATTATCATCTGATGACAAAATAATTGAACCAGCAAACATTAAT
    TGCCCAGGTCAAATTGTTGTTTCAGGTCACAAAGCTTTAATTGATGAGCTAGTAGAAAAA
    GGTAAATCATTAGGTGCAAAACGTGTCATGCCTTTAGCAGTATCTGGACCATTCCATTCA
    TCGCTAATGAAAGTGATTGAAGAAGATTTTTCAAGTTACATTAATCAATTTGAATGGCGT
    GATGCTAAGTTTCCTGTAGTTCAAAATGTAAATGCGCAAGGTGAAACTGACAAAGAAGTA
    ATTAAATCTAATATGGTCAAGCAATTATATTCACCAGTACAATTCATTAACTCAACAGAA
    TGGCTAATAGACCAAGGTGTTGATCATTTTATTGAAATTGGTCCTGGAAAAGTTTTATCT
    GGCTTAATTAAAAAAATAAATAGAGATGTTAAGTTAACATCAATTCAAACTTTAGAAGAT
    GTGAAAGGATGGAATGAAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1073 [new locus tag: SA_RS06080 ]
  • symbol: FabD
  • description: malonyl CoA-ACP transacylase
  • length: 308
  • theoretical pI: 4.58365
  • theoretical MW: 33649.1
  • GRAVY: -0.148052

Function[edit source | edit]

  • reaction:
    EC 2.3.1.39?  ExPASy
    [Acyl-carrier-protein] S-malonyltransferaseMalonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismBiosynthesismalonyl CoA-acyl carrier protein transacylase (TIGR00128; EC 2.3.1.39; HMM-score: 327.5)
    malonate decarboxylase, epsilon subunit (TIGR03131; HMM-score: 201.5)
    polyketide-type polyunsaturated fatty acid synthase PfaA (TIGR02813; HMM-score: 117.3)
    PfaB family protein (TIGR02816; HMM-score: 16.4)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsFatty acidsFatty Acid Biosynthesis FASII Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) 
    COG1399 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) 
  • PFAM:
    Patatin (CL0323) Acyl_transf_1; Acyl transferase domain (PF00698; HMM-score: 123.4)
    no clan definedV-ATPase_C; V-ATPase subunit C (PF03223; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.174
    • Ymax_pos: 20
    • Cmax: 0.144
    • Cmax_pos: 30
    • Smax: 0.297
    • Smax_pos: 18
    • Smean: 0.193
    • D: 0.181
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDILETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVLSFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANINCPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFEWRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPGKVLSGLIKKINRDVKLTSIQTLEDVKGWNEND

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SA1071 (FapR*)
    FapR* (TF) important in Fatty acid biosynthesisRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J. Microbiol. Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)