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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1218 [new locus tag: SA_RS06920 ]
  • pan locus tag?: SAUPAN003794000
  • symbol: pstB
  • pan gene symbol?: pstB
  • synonym:
  • product: phosphate transporter ATP-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1218 [new locus tag: SA_RS06920 ]
  • symbol: pstB
  • product: phosphate transporter ATP-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1391139..1391990
  • length: 852
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCGCAAACACTTGCACAAACTAAACAAATATCTCAAAGTCATACGTTTGATGTCTCA
    CAAAGTCATCATAAAACACCAGATGATACAAACTCACATTCTGTTATATATTCAACACAA
    AATTTAGACTTATGGTATGGCGAAAATCATGCATTACAAAATATTAATTTAGATATTTAT
    GAAAACCAAATTACTGCCATTATAGGTCCATCTGGTTGTGGCAAATCAACATATATTAAA
    ACTTTGAATCGAATGGTTGAGTTAGTACCTTCTGTGAAAACAGCTGGTAAAATATTATAT
    CGAGATCAAGACATTTTTGATCAAAAATATTCTAAAGAACAATTACGTACAAATGTGGGC
    ATGGTCTTTCAACAACCTAATCCATTTCCAAAATCAATATACGATAATATTACTTACGGT
    CCAAAGATTCACGGTATTAAAAATAAAAAAGTTCTTGATGAAATCGTTGAGAAATCATTA
    CGTGGCGCTGCAATTTGGGATGAATTAAAGGATAGGTTGCACACAAATGCATATAGTTTA
    TCCGGTGGGCAACAACAACGTGTTTGTATCGCGCGTTGTTTAGCAATTGAACCTGAAGTC
    ATTTTAATGGATGAACCGACATCAGCATTAGATCCAATCTCAACATTAAGAGTAGAAGAG
    TTGGTTCAAGAACTAAAAGAAAAGTATACAATTATTATGGTTACACATAATATGCAACAA
    GCAGCTCGTGTATCAGATAAAACTGCATTTTTCTTAAATGGTTATGTCAATGAATATGAT
    GATACTGATAAAATTTTCTCTAACCCATCAAACAAGAAAACAGAAGATTATATTTCAGGA
    AGGTTTGGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    852

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1218 [new locus tag: SA_RS06920 ]
  • symbol: PstB
  • description: phosphate transporter ATP-binding protein
  • length: 283
  • theoretical pI: 6.51418
  • theoretical MW: 32154.1
  • GRAVY: -0.537809

Function[edit source | edit]

  • reaction:
    EC 3.6.3.27?  ExPASy
    Phosphate-transporting ATPaseATP + H2O + phosphate(Out) = ADP + phosphate + phosphate(In)
  • TIGRFAM:
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 427)
    MetabolismTransport and binding proteinsUnknown substrateenergy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 172.3)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesglycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 166.2)
    MetabolismTransport and binding proteinsUnknown substrateenergy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 150)
    ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 141.2)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesputative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 136.1)
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 134.2)
    D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 126.6)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 126.2)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 125.1)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminescholine ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 123.5)
    2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 118.9)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel import ATP-binding protein NikD (TIGR02770; HMM-score: 118.8)
    Cellular processesCellular processesCell divisioncell division ATP-binding protein FtsE (TIGR02673; HMM-score: 116.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminespolyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 116.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesurea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 115.5)
    Cellular processesCellular processesToxin production and resistanceputative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 114.3)
    MetabolismTransport and binding proteinsUnknown substrateputative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 114.3)
    Cellular processesCellular processesPathogenesistype I secretion system ATPase (TIGR03375; HMM-score: 114.2)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion system ATPase (TIGR03375; HMM-score: 114.2)
    MetabolismTransport and binding proteinsOtherdaunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 112.9)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharideslipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 110.3)
    MetabolismTransport and binding proteinsOtherlipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 110.3)
    MetabolismTransport and binding proteinsOtherantigen peptide transporter 2 (TIGR00958; HMM-score: 109.1)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 109)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesLPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 108.5)
    MetabolismTransport and binding proteinsOtherLPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 108.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 108.3)
    ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 106.7)
    ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 103.9)
    thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 102.3)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)
    Genetic information processingProtein fateProtein modification and repairABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)
    MetabolismTransport and binding proteinsOtherABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)
    MetabolismTransport and binding proteinsOtherthiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 100.9)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion system ATPase (TIGR01846; HMM-score: 100.1)
    Cellular processesCellular processesBiosynthesis of natural productsNHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 98.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesNHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 98.9)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 98.5)
    MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 96.7)
    Genetic information processingProtein fateProtein and peptide secretion and traffickinglipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 96.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesurea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 96.3)
    gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 89.1)
    Cellular processesCellular processesBiosynthesis of natural productsNHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 88.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesNHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 88.9)
    lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 86.6)
    MetabolismTransport and binding proteinsAnionsnitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 85)
    MetabolismTransport and binding proteinsOthernitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 85)
    MetabolismEnergy metabolismMethanogenesismethyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 79.6)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion system ATPase (TIGR01842; HMM-score: 79.2)
    MetabolismTransport and binding proteinsUnknown substrateanchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 77.9)
    proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 75.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsglucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 74.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherFeS assembly ATPase SufC (TIGR01978; HMM-score: 69.6)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 68.5)
    MetabolismTransport and binding proteinsOtherrim ABC transporter (TIGR01257; HMM-score: 67.6)
    MetabolismCentral intermediary metabolismPhosphorus compoundsphosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 64.9)
    Cellular processesCellular processesOthernodulation ABC transporter NodI (TIGR01288; HMM-score: 59.7)
    MetabolismTransport and binding proteinsOthernodulation ABC transporter NodI (TIGR01288; HMM-score: 59.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 59.2)
    ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 59.1)
    MetabolismTransport and binding proteinsOtherpigment precursor permease (TIGR00955; HMM-score: 57.8)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingheme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 57.6)
    MetabolismTransport and binding proteinsOtherheme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 57.6)
    MetabolismTransport and binding proteinsAnionscystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 47.2)
    MetabolismTransport and binding proteinsOthermulti drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 43.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 41.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsperoxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 39.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminescyclic peptide transporter (TIGR01194; HMM-score: 39.5)
    MetabolismTransport and binding proteinsOthercyclic peptide transporter (TIGR01194; HMM-score: 39.5)
    MetabolismTransport and binding proteinsOtherpleiotropic drug resistance family protein (TIGR00956; HMM-score: 28.4)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02168; HMM-score: 27.2)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02168; HMM-score: 27.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repaircheckpoint protein rad24 (TIGR00602; HMM-score: 21.4)
    Genetic information processingProtein synthesisTranslation factorsribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 14.4)
    MetabolismCentral intermediary metabolismPhosphorus compoundsphosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN (TIGR02322; HMM-score: 13.9)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion protein EccCb (TIGR03925; HMM-score: 12.9)
    Ti-type conjugative transfer relaxase TraA (TIGR02768; HMM-score: 10.5)
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryHigh affinity phosphate transporter and control of PHO regulon Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) 
    Phosphorus Metabolism - no subcategoryPhosphate metabolism Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) 
  • PFAM:
    P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 117.3)
    SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 39.4)
    AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 37.9)
    AAA_15; AAA ATPase domain (PF13175; HMM-score: 24.7)
    AAA_22; AAA domain (PF13401; HMM-score: 21.3)
    Rad17; Rad17 cell cycle checkpoint protein (PF03215; HMM-score: 20.6)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 20.5)
    AAA_23; AAA domain (PF13476; HMM-score: 19.6)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 19.2)
    AAA_10; AAA-like domain (PF12846; HMM-score: 17.1)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 17.1)
    AAA_30; AAA domain (PF13604; HMM-score: 15.7)
    PRK; Phosphoribulokinase / Uridine kinase family (PF00485; HMM-score: 15.7)
    AAA_18; AAA domain (PF13238; HMM-score: 15.7)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.3)
    AAA_14; AAA domain (PF13173; HMM-score: 14.8)
    AAA_28; AAA domain (PF13521; HMM-score: 14.5)
    AAA_24; AAA domain (PF13479; HMM-score: 14.3)
    Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 14.2)
    G-alpha; G-protein alpha subunit (PF00503; HMM-score: 13.9)
    AAA_19; AAA domain (PF13245; HMM-score: 13.7)
    PhoH; PhoH-like protein (PF02562; HMM-score: 13.4)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 13.1)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.8)
    Adeno_IVa2; Adenovirus IVa2 protein (PF02456; HMM-score: 12.7)
    ABC_ATPase; Predicted ATPase of the ABC class (PF09818; HMM-score: 12.6)
    no clan definedVF530; DNA-binding protein VF530 (PF09905; HMM-score: 12.5)
    P-loop_NTPase (CL0023) AAA_33; AAA domain (PF13671; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA1528hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.187
    • Ymax_pos: 23
    • Cmax: 0.136
    • Cmax_pos: 23
    • Smax: 0.409
    • Smax_pos: 19
    • Smean: 0.22
    • D: 0.2
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDLWYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRDQDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSLRGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRVEELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTEDYISGRFG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]