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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1283 [new locus tag: SA_RS07270 ]
  • pan locus tag?: SAUPAN003910000
  • symbol: pbp2
  • pan gene symbol?: pbp2
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1283 [new locus tag: SA_RS07270 ]
  • symbol: pbp2
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1486656..1488839
  • length: 2184
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGACGGAAAACAAAGGATCTTCTCAGCCTAAGAAAAACGGTAATAATGGTGGGAAATCC
    AACTCAAAAAAGAATAGAAATGTGAAGAGAACGATTATTAAGATTATTGGCTTCATGATT
    ATTGCATTTTTCGTTGTTCTTTTACTAGGTATCTTATTGTTTGCTTATTATGCTTGGAAA
    GCACCTGCTTTTACCGAAGCTAAATTACAAGATCCGATTCCTGCAAAGATATATGACAAG
    AACGGAGAACTTGTTAAAACATTAGATAATGGCCAAAGACATGAGCATGTAAATTTAAAA
    GACGTGCCGAAATCAATGAAAGACGCAGTACTTGCAACTGAAGACAATCGTTTCTACGAA
    CATGGCGCACTTGATTATAAACGTTTATTCGGTGCAATTGGTAAGAACTTGACTGGTGGA
    TTTGGTTCTGAAGGTGCCTCAACATTAACACAACAAGTTGTTAAAGATGCATTTTTATCA
    CAACATAAATCTATTGGACGTAAAGCTCAAGAAGCATACTTATCATATCGTTTAGAACAA
    GAGTATAGTAAAGATGATATCTTCCAAGTATATCTAAACAAAATTTACTGTTCTGATGGC
    GTAACAGGTATTAAAGCTGCTGCTAAGTATTACTTTAATAAAGATTTAAAAGATTTAAAC
    TTAGCGGAAGAAGCTTATTTAGCCGGTTTACCTCAGGTTCCAAACAACTATAATATTTAT
    GATCATCCAAAAGCTGCTGAAGATCGTAAAAACACTGTTTTATACTTAATGCATTATCAT
    AAACGCATTACAGATAAACAGTGGGAAGATGCTAAGAAAATCGATTTAAAAGCGAACTTA
    GTAAATCGTACTCCTGAAGAACGTCAAAACATTGATACAAATCAAGATTCTGAGTATAAT
    TCATACGTTAACTTTGTGAAATCTGAATTAATGAATAATAAAGCATTCAAAGATGAAAAT
    TTAGGTAATGTATTACAAAGTGGTATTAAAATTTATACAAACATGGATAAAGATGTTCAA
    AAAACATTACAAAATGATGTTGATAATGGTAGCTTCTACAAGAATAAAGACCAACAAGTT
    GGTGCAACGATTCTTGATAGTAAAACTGGTGGTTTAGTTGCTATATCTGGTGGACGTGAT
    TTCAAAGACGTCGTTAACAGAAACCAAGCAACAGATCCTCACCCTACTGGTTCATCTTTA
    AAACCTTTCTTAGCGTATGGACCTGCCATTGAAAATATGAAATGGGCAACAAACCATGCG
    ATTCAAGATGAATCTTCATATCAAGTTGACGGTTCTACATTTAGAAACTATGATACGAAG
    AGTCACGGTACTGTATCTATTTATGATGCTTTACGACAAAGTTTCAATATCCCAGCTTTA
    AAAGCATGGCAATCAGTTAAGCAAAATGCTGGTAACGATGCACCTAAGAAATTCGCTGCC
    AAACTTGGCTTAAACTACGAAGGCGATATTGGTCCATCTGAAGTACTTGGTGGTTCTGCT
    TCAGAATTCTCACCAACACAATTAGCATCAGCATTTGCTGCAATCGCTAACGGTGGTACT
    TATAACAACGCGCATTCAATTCAAAAAGTAGTTACTCGTGACGGTGAAACAATCGAATAC
    GATCATACTAGCCATAAAGCGATGAGTGATTACACTGCATACATGTTAGCTGAGATGCTA
    AAAGGTACATTTAAACCATATGGTTCTGCATATGGCCATGGTGTATCTGGAGTAAATATG
    GGTGCTAAGACAGGTACTGGTACTTACGGTGCTGAAACTTATTCACAATATAATTTACCT
    GATAATGCAGCGAAAGACGTGTGGATTAACGGCTTTACACCTCAATACACTATGTCAGTG
    TGGATGGGCTTCAGTAAAGTTAAACAATATGGTGAAAACTCATTTGTGGGACATAGCCAA
    CAAGAATATCCACAGTTCTTATATGAAAATGTGATGTCAAAAATTTCATCTAGAGATGGC
    GAAGACTTTAAACGTCCTAGCTCAGTAAGTGGTAGTATCCCATCAATCAATGTTTCTGGT
    AGTCAAGATAACAACACTACAAATCGTAGTACACACGGTGGTAGTGACACATCAGCAAAC
    AGCAGTGGTACTGCACAATCAAATAACAATACTAGATCTCAACAATCTAGAAACAGCGGT
    GGATTAACAGGTATATTCAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2184

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1283 [new locus tag: SA_RS07270 ]
  • symbol: Pbp2
  • description: hypothetical protein
  • length: 727
  • theoretical pI: 9.07362
  • theoretical MW: 80365.8
  • GRAVY: -0.676616

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanpenicillin-binding protein, 1A family (TIGR02074; HMM-score: 451.6)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanpenicillin-binding protein 1B (TIGR02071; HMM-score: 244.2)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanpenicillin-binding protein 1C (TIGR02073; HMM-score: 231)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanmonofunctional biosynthetic peptidoglycan transglycosylase (TIGR02070; EC 2.4.2.-; HMM-score: 120.7)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanpenicillin-binding protein 2 (TIGR03423; HMM-score: 68)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanstage V sporulation protein D (TIGR02214; HMM-score: 25.9)
    Cellular processesCellular processesSporulation and germinationstage V sporulation protein D (TIGR02214; HMM-score: 25.9)
  • TheSEED:  
    Control of cell elongation - division cycle in Bacilli Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) / Penicillin-binding protein 1A/1B (PBP1) 
    Peptidoglycan Biosynthesis Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) / Penicillin-binding protein 1A/1B (PBP1) 
  • PFAM:
    Lysozyme (CL0037) Transgly; Transglycosylase (PF00912; HMM-score: 199.1)
    Beta-lactamase (CL0013) Transpeptidase; Penicillin binding protein transpeptidase domain (PF00905; HMM-score: 98.2)
    no clan definedSigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 14.1)
    DUF3671; Protein of unknown function (PF12420; HMM-score: 13.7)
    Pox_EPC_I2-L1; Poxvirus entry protein complex L1 and I2 (PF12575; HMM-score: 11.5)
    Orf78; Orf78 (ac78) (PF06024; HMM-score: 8.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA0708(secA)preprotein translocase subunit SecA  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 5
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.149
    • Ymax_pos: 11
    • Cmax: 0.202
    • Cmax_pos: 61
    • Smax: 0.257
    • Smax_pos: 7
    • Smean: 0.215
    • D: 0.175
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

  • GI: 15927031 NCBI
  • RefSeq: NP_374564 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFFVVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDVPKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLSQHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYCSDGVTGIKAAAKYYFNKDLKDLNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDLKANLVNRTPEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTNMDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATDPHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDALRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQLASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKPYGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGFSKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQDNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]