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NCBI: 26-AUG-2013

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1289 [new locus tag: SA_RS07300 ]
  • pan locus tag?: SAUPAN003916000
  • symbol: SA1289
  • pan gene symbol?: birA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: SA1289 [new locus tag: SA_RS07300 ]
  • symbol: SA1289
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1495745..1496716
  • length: 972
  • essential: no DEG other strains

⊟Accession numbers[edit source | edit]

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCAAAATATAGTCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATA
    TCTGGACAAAGCATTGCGGAATCACTTAATATTTCACGCACTGCAGTAAAAAAGGTGATT
    GACCAATTAAAGTTAGAGGGATGTAAAATTGATTCAGTAAATCATAAAGGGCATTTATTA
    CAACAACTCCCAGATATTTGGTATCAGGGTATAATAGACCAATATACAAAAAGTTCTGCT
    TTGTTTGATTTTAGTGAAGTATACGATTCAATAGATTCTACACAACTTGCTGCGAAAAAG
    TCACTTGTTGGAAATCAATCTTCATTTTTTATCTTGAGTGATGAACAAACGAAAGGTCGT
    GGGCGATTTAATAGACATTGGAGTTCTTCAAAAGGGCAAGGACTTTGGATGTCTGTCGTG
    TTAAGACCTAACGTTGCATTCTCAATGATATCTAAATTTAATTTATTTATTGCATTAGGG
    ATAAGAGATGCGATTCAACATTTTAGTCAAGATGAAGTCAAAGTGAAATGGCCGAATGAT
    ATATATATTGATAATGGTAAAGTGTGTGGTTTCTTAACTGAAATGGTTGCTAATAATGAT
    GGTATAGAAGCAATAATATGTGGTATAGGTATTAATTTGACGCAACAACTAGAAAACTTT
    GATGAAAGTATTAGACATAGAGCAACAAGTATACAATTACATGATAAAAATAAATTAGAT
    AGATATCAATTTTTAGAGAGATTACTTCAAGAAATTGAAAAAAGATATAATCAATTTTTA
    ACGTTACCTTTTTCTGAAATTCGTGAAGAATATATTGCAGCTTCTAATATTTGGAATAGA
    ACGTTGCTATTTACAGAAAATGATAAACAGTTTAAAGGACAAGCAATTGATTTAGATTAC
    GATGGCTATCTAATTGTTAGAGATGAAGCGGGTGAATCACACCGTTTAATTAGTGCAGAT
    ATAGATTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    972

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: SA1289 [new locus tag: SA_RS07300 ]
  • symbol: SA1289
  • description: hypothetical protein
  • length: 323
  • theoretical pI: 6.1777
  • theoretical MW: 37141.8
  • GRAVY: -0.372446

⊟Function[edit source | edit]

  • reaction:
    EC 6.3.4.15?  ExPASy
    Biotin--[acetyl-CoA-carboxylase] ligaseATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
  • TIGRFAM:
    Genetic information processingProtein fateProtein modification and repairbiotin--[acetyl-CoA-carboxylase] ligase (TIGR00121; EC 6.3.4.15; HMM-score: 179.5)
    and 4 more
    Signal transductionRegulatory functionsDNA interactionsbiotin operon repressor (TIGR00122; HMM-score: 32.2)
    Signal transductionRegulatory functionsDNA interactionsTyrR family helix-turn-helix domain (TIGR04381; HMM-score: 15.1)
    Unknown functionGeneralRrf2 family protein (TIGR00738; HMM-score: 13.6)
    Signal transductionRegulatory functionsDNA interactionsCRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 11.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsBiotinBiotin biosynthesis Biotin operon repressor
    and 5 more
    Biotin--protein ligase (EC 6.3.4.9)(EC 6.3.4.10)(EC 6.3.4.11)(EC 6.3.4.15)
    Biotin--protein ligase (EC 6.3.4.9, EC 6.3.4.10, EC 6.3.4.11, EC 6.3.4.15)
    Biotin synthesis & utilization Biotin operon repressor
    Biotin--protein ligase (EC 6.3.4.9)(EC 6.3.4.10)(EC 6.3.4.11)(EC 6.3.4.15)
    MiscellaneousPlant-Prokaryote comparative genomicsIojapClusters Biotin--protein ligase (EC 6.3.4.9)(EC 6.3.4.10)(EC 6.3.4.11)(EC 6.3.4.15)
  • PFAM:
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 53.4)
    tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 48.7)
    and 12 more
    BPL_LplA_LipB_2; Biotin/lipoate A/B protein ligase family (PF16917; HMM-score: 21.8)
    TRB (CL0206) BPL_C; Biotin protein ligase C terminal domain (PF02237; HMM-score: 19.7)
    HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19.4)
    Rrf2; Transcriptional regulator (PF02082; HMM-score: 17.4)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 15.9)
    MarR; MarR family (PF01047; HMM-score: 14.8)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14)
    RPA_C; Replication protein A C terminal (PF08784; HMM-score: 13.6)
    Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 13.5)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.4)
    no clan definedPulG; Type II secretory pathway pseudopilin (PF11773; HMM-score: 12.8)
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 11.7)

⊟Structure, modifications & interactions[edit source | edit]

⊟Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.123
    • Ymax_pos: 27
    • Cmax: 0.124
    • Cmax_pos: 27
    • Smax: 0.183
    • Smax_pos: 35
    • Smean: 0.125
    • D: 0.124
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit source | edit]

⊟Protein sequence[edit source | edit]

  • MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF

⊟Experimental data[edit source | edit]

  • experimentally validated: no data available

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

⊟Regulation[edit source | edit]

  • regulator:

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

  1. ↑ Jingheng Wang, Dorothy Beckett
    A conserved regulatory mechanism in bifunctional biotin protein ligases.
    Protein Sci.: 2017, 26(8);1564-1573
    [PubMed:28466579] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit source | edit]

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