NCBI: 26-AUG-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus N315
- locus tag: SA1289 [new locus tag: SA_RS07300 ]
- pan locus tag?: SAUPAN003916000
- symbol: SA1289
- pan gene symbol?: birA
- synonym:
- product: hypothetical protein
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SA1289 [new locus tag: SA_RS07300 ]
- symbol: SA1289
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1495745..1496716
- length: 972
- essential: no DEG other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 1124127 NCBI
- RefSeq: NP_374570 NCBI
- MicrobesOnline: 103596 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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961ATGTCAAAATATAGTCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATA
TCTGGACAAAGCATTGCGGAATCACTTAATATTTCACGCACTGCAGTAAAAAAGGTGATT
GACCAATTAAAGTTAGAGGGATGTAAAATTGATTCAGTAAATCATAAAGGGCATTTATTA
CAACAACTCCCAGATATTTGGTATCAGGGTATAATAGACCAATATACAAAAAGTTCTGCT
TTGTTTGATTTTAGTGAAGTATACGATTCAATAGATTCTACACAACTTGCTGCGAAAAAG
TCACTTGTTGGAAATCAATCTTCATTTTTTATCTTGAGTGATGAACAAACGAAAGGTCGT
GGGCGATTTAATAGACATTGGAGTTCTTCAAAAGGGCAAGGACTTTGGATGTCTGTCGTG
TTAAGACCTAACGTTGCATTCTCAATGATATCTAAATTTAATTTATTTATTGCATTAGGG
ATAAGAGATGCGATTCAACATTTTAGTCAAGATGAAGTCAAAGTGAAATGGCCGAATGAT
ATATATATTGATAATGGTAAAGTGTGTGGTTTCTTAACTGAAATGGTTGCTAATAATGAT
GGTATAGAAGCAATAATATGTGGTATAGGTATTAATTTGACGCAACAACTAGAAAACTTT
GATGAAAGTATTAGACATAGAGCAACAAGTATACAATTACATGATAAAAATAAATTAGAT
AGATATCAATTTTTAGAGAGATTACTTCAAGAAATTGAAAAAAGATATAATCAATTTTTA
ACGTTACCTTTTTCTGAAATTCGTGAAGAATATATTGCAGCTTCTAATATTTGGAATAGA
ACGTTGCTATTTACAGAAAATGATAAACAGTTTAAAGGACAAGCAATTGATTTAGATTAC
GATGGCTATCTAATTGTTAGAGATGAAGCGGGTGAATCACACCGTTTAATTAGTGCAGAT
ATAGATTTTTAA60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SA1289 [new locus tag: SA_RS07300 ]
- symbol: SA1289
- description: hypothetical protein
- length: 323
- theoretical pI: 6.1777
- theoretical MW: 37141.8
- GRAVY: -0.372446
⊟Function[edit source | edit]
- reaction: EC 6.3.4.15? ExPASyBiotin--[acetyl-CoA-carboxylase] ligaseATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
- TIGRFAM: Protein fateProtein modification and repairbiotin--[acetyl-CoA-carboxylase] ligase (TIGR00121; EC 6.3.4.15; HMM-score: 179.5)Regulatory functionsDNA interactionsbiotin operon repressor (TIGR00122; HMM-score: 32.2)Regulatory functionsDNA interactionsTyrR family helix-turn-helix domain (TIGR04381; HMM-score: 15.1)Unknown functionGeneralRrf2 family protein (TIGR00738; HMM-score: 13.6)Regulatory functionsDNA interactionsCRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 11.5)
- TheSEED:
- PFAM: HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 53.4)tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 48.7)BPL_LplA_LipB_2; Biotin/lipoate A/B protein ligase family (PF16917; HMM-score: 21.8)TRB (CL0206) BPL_C; Biotin protein ligase C terminal domain (PF02237; HMM-score: 19.7)HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19.4)Rrf2; Transcriptional regulator (PF02082; HMM-score: 17.4)HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 15.9)MarR; MarR family (PF01047; HMM-score: 14.8)Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14)RPA_C; Replication protein A C terminal (PF08784; HMM-score: 13.6)Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 13.5)HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.4)no clan definedPulG; Type II secretory pathway pseudopilin (PF11773; HMM-score: 12.8)HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 11.7)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular Possibility: 1
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.123
- Ymax_pos: 27
- Cmax: 0.124
- Cmax_pos: 27
- Smax: 0.183
- Smax_pos: 35
- Smean: 0.125
- D: 0.124
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- MicrobesOnline: dinG < SA1289 < SA1290 < SA1291
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]