NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1289 [new locus tag: SA_RS07300 ]
• pan locus tag^?: SAUPAN003916000
• symbol: SA1289
• pan gene symbol^?: birA
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: SA1289 [new locus tag: SA_RS07300 ]
• symbol: SA1289
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1495745..1496716
• length: 972
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124127 NCBI
• RefSeq: NP_374570 NCBI
• MicrobesOnline: 103596 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  961
  
  ATGTCAAAATATAGTCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATA
  TCTGGACAAAGCATTGCGGAATCACTTAATATTTCACGCACTGCAGTAAAAAAGGTGATT
  GACCAATTAAAGTTAGAGGGATGTAAAATTGATTCAGTAAATCATAAAGGGCATTTATTA
  CAACAACTCCCAGATATTTGGTATCAGGGTATAATAGACCAATATACAAAAAGTTCTGCT
  TTGTTTGATTTTAGTGAAGTATACGATTCAATAGATTCTACACAACTTGCTGCGAAAAAG
  TCACTTGTTGGAAATCAATCTTCATTTTTTATCTTGAGTGATGAACAAACGAAAGGTCGT
  GGGCGATTTAATAGACATTGGAGTTCTTCAAAAGGGCAAGGACTTTGGATGTCTGTCGTG
  TTAAGACCTAACGTTGCATTCTCAATGATATCTAAATTTAATTTATTTATTGCATTAGGG
  ATAAGAGATGCGATTCAACATTTTAGTCAAGATGAAGTCAAAGTGAAATGGCCGAATGAT
  ATATATATTGATAATGGTAAAGTGTGTGGTTTCTTAACTGAAATGGTTGCTAATAATGAT
  GGTATAGAAGCAATAATATGTGGTATAGGTATTAATTTGACGCAACAACTAGAAAACTTT
  GATGAAAGTATTAGACATAGAGCAACAAGTATACAATTACATGATAAAAATAAATTAGAT
  AGATATCAATTTTTAGAGAGATTACTTCAAGAAATTGAAAAAAGATATAATCAATTTTTA
  ACGTTACCTTTTTCTGAAATTCGTGAAGAATATATTGCAGCTTCTAATATTTGGAATAGA
  ACGTTGCTATTTACAGAAAATGATAAACAGTTTAAAGGACAAGCAATTGATTTAGATTAC
  GATGGCTATCTAATTGTTAGAGATGAAGCGGGTGAATCACACCGTTTAATTAGTGCAGAT
  ATAGATTTTTAA
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⊟Protein

⊟General

• locus tag: SA1289 [new locus tag: SA_RS07300 ]
• symbol: SA1289
• description: hypothetical protein
• length: 323
• theoretical pI: 6.1777
• theoretical MW: 37141.8
• GRAVY: -0.372446

⊟Function

• reaction:
  EC 6.3.4.15?  ExPASy
  Biotin--[acetyl-CoA-carboxylase] ligaseATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
• TIGRFAM:
  Genetic information processingProtein fateProtein modification and repairbiotin--[acetyl-CoA-carboxylase] ligase (TIGR00121; EC 6.3.4.15; HMM-score: 179.5)
  and 4 more
  Signal transductionRegulatory functionsDNA interactionsbiotin operon repressor (TIGR00122; HMM-score: 32.2)
  Signal transductionRegulatory functionsDNA interactionsTyrR family helix-turn-helix domain (TIGR04381; HMM-score: 15.1)
  Unknown functionGeneralRrf2 family protein (TIGR00738; HMM-score: 13.6)
  Signal transductionRegulatory functionsDNA interactionsCRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 11.5)
• TheSEED:  
  Biotin biosynthesis Biotin operon repressor / Biotin--protein ligase (EC 6.3.4.9)(EC 6.3.4.10)(EC 6.3.4.11)(EC 6.3.4.15) 
• PFAM:
  HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 53.4)
  tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 48.7)
  and 12 more
  BPL_LplA_LipB_2; Biotin/lipoate A/B protein ligase family (PF16917; HMM-score: 21.8)
  TRB (CL0206) BPL_C; Biotin protein ligase C terminal domain (PF02237; HMM-score: 19.7)
  HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19.4)
  Rrf2; Transcriptional regulator (PF02082; HMM-score: 17.4)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 15.9)
  MarR; MarR family (PF01047; HMM-score: 14.8)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14)
  RPA_C; Replication protein A C terminal (PF08784; HMM-score: 13.6)
  Put_DNA-bind_N; Putative DNA-binding protein N-terminus (PF06971; HMM-score: 13.5)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.4)
  no clan definedPulG; Type II secretory pathway pseudopilin (PF11773; HMM-score: 12.8)
  HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 11.7)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors: Biotin
• genes regulated by SA1289 (BirA*), TF important in Biotin biosynthesisRegPrecise

  repression

  vraA > vraB > vraC > SA0536

  SA2076 < SA2077

  SA2210 < SA2211 < SA2212 < bioB < bioA < bioD
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.123
  • Ymax_pos: 27
  • Cmax: 0.124
  • Cmax_pos: 27
  • Smax: 0.183
  • Smax_pos: 35
  • Smean: 0.125
  • D: 0.124
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927037 NCBI
• RefSeq: NP_374570 NCBI
• UniProt: A0A0H3JU61 UniProt

⊟Protein sequence

• MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLKLEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSLVGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALGIRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLENFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASNIWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dinG < SA1289 < SA1290 < SA1291

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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