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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2180 [new locus tag: SA_RS12510 ]
  • pan locus tag?: SAUPAN005923000
  • symbol: SA2180
  • pan gene symbol?: nreB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2180 [new locus tag: SA_RS12510 ]
  • symbol: SA2180
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2448396..2449430
  • length: 1035
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTAATGAGGACAGTATACAGTTAGATACTTTATTGAAAAAATATTATGAGCATTCA
    ATCGAAAAGATAGTTTTTGCTGATGATAACGGCAAAATTATTGCAATGAATGATGCTGCT
    AAAGATATATTATCTGAAGAAGATAATTACAGTGCTGTGGCGAATGCGATTTGTCACAGA
    TGTGAAGGGTATACAAATGCCTATGATGTACAATCATGTAAAGACTGTTTCTTAGAATCG
    ATGCAAGTACAAGCTACGAATTTCCAAGTATTTATGAAGACGAAAGATCAAAAAGTGATG
    CCATTTACAGCAACATATCAGTTGATTGATCAAGACAGGGGAATTCATGCATTTACGCTT
    CAAAATGTTTCATCACAAATTGAACAGCAAGAGAAATTACATCAACAACGTATGATGCGT
    AAAACAATCTCAGCCCAAGAAAATGAAAGAAAGCGAATTTCTAGAGAGTTACATGATAGT
    GTGATTCAAGAAATGCTCAATGTAGATGTTCAATTGAGATTATTAAAATATCAAGAAGAT
    ACAACTAAGCTTTTGGAAGACGCAGAAAATATTGAATATATTGTCGCTAAATTAATTGAT
    GATATACGTAATATGTCGGTTGAATTAAGACCCGCTTCTTTGGATGATCTAGGCCTTGAA
    GCAGCTTTTAAATCATATTTTAAACAGTTTGAAGAAAATTATGGAATAAAAATCATATAT
    ACATCAAATATTAAAAATACTCGCTTTGATAGTGATATTGAAACGGTTGTTTATCGAGTA
    GTGCAAGAAGCTATTCTAAATGCGTTGAAATACGCTGATGTAAATGAAATAAATGTAGGT
    ATCCGTCAAACTGGGCGCCATTTGGTAGCAGAAGTGATAGATGCGGGAAATGGTTTTGAT
    CCAAGTTCTAAGCCTAAAGGTTCTGGACTCGGTTTATATGGAATGAATGAGCGAGCTGAG
    TTAGTCAGCGGTAGCGTCAATATTGAAACGAAAATTGGTGAGGGTACCAATGTTACATTG
    AACATTCCAATTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1035

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2180 [new locus tag: SA_RS12510 ]
  • symbol: SA2180
  • description: hypothetical protein
  • length: 344
  • theoretical pI: 4.65774
  • theoretical MW: 39120
  • GRAVY: -0.409302

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)
    Signal transductionRegulatory functionsSmall molecule interactionsPAS domain S-box protein (TIGR00229; HMM-score: 17.2)
  • TheSEED:  
    Nitrate and nitrite ammonification Two-component system sensor histidine kinase 
  • PFAM:
    His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 53.4)
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 38.7)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 23.6)
    PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 22)
    UBA (CL0214) WIYLD; Ubiquitin-binding WIYLD domain (PF10440; HMM-score: 13.6)
    no clan definedDUF4974; Domain of unknown function (DUF4974) (PF16344; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 28
    • Cmax: 0.225
    • Cmax_pos: 28
    • Smax: 0.11
    • Smax_pos: 27
    • Smean: 0.086
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDILSEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPFTATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDSVIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLGLEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNEINVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGEGTNVTLNIPI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: SA1851 (Rex*), SA2179 (NreC*)
    Rex* (TF) important in Energy metabolismRegPrecise
    NreC* (TF) important in Nitrate and nitrite respirationRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]