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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2185 [new locus tag: SA_RS12535 ]
  • pan locus tag?: SAUPAN005928000
  • symbol: narG
  • pan gene symbol?: narG
  • synonym:
  • product: respiratory nitrate reductase subunit alpha

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2185 [new locus tag: SA_RS12535 ]
  • symbol: narG
  • product: respiratory nitrate reductase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 2452713..2456402
  • length: 3690
  • essential: yes DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGAAAATTTGGATTGAATTTCTTTAAGCCAACAGAAAAATTTAATGGGAATTGGTCG
    ATCCTAGAAAGTAAAAGTAGAGAATGGGAAAAAATGTACAGAGAACGTTGGAGCCACGAT
    AAAGAAGTAAGAACAACACATGGTGTTAACTGTACAGGCTCATGTTCTTGGAAAGTATTT
    GTGAAAAATGGTGTGATTACCTGGGAAAATCAACAAACTGACTATCCAAGTTGTGGTCCG
    GATATGCCTGAATATGAACCGAGAGGATGTCCACGAGGTGCGTCATTCTCTTGGTATGAA
    TACAGTCCGCTTCGAATCAAATATCCATATATTCGTGGAAAACTCTGGGATTTATGGACT
    GAAGCATTAGAAGAAAACTATGGTAATCGCGTTGCTGCATGGGCGTCTATTGTTGAAAAT
    GAAGACAAAGCCAAACAATATAAGCAAGCCCGAGGTATGGGAGGGCACGTGCGTTCAAAT
    TGGAAAGACGTTACAGAGATAATCGCAGCACAATTACTGTATACAATAAAAAAATATGGT
    CCAGATCGAATCGCAGGATTTACACCTATTCCAGCGATGTCAATGATTAGTTATGCAGCA
    GGTGCTCGATTCATCAATTTGCTTGGTGGTGAAATGCTTAGTTTTTATGACTGGTATGCA
    GATTTACCACCTGCCTCTCCACAAATTTGGGGAGAGCAAACAGATGTGCCTGAATCAAGT
    GACTGGTATAACGCATCATACATTATTATGTGGGGCTCTAATGTACCTTTAACACGTACT
    CCGGATGCACATTTTATGACAGAAGTCCGCTATAAAGGTACAAAAGTCATTTCAGTAGCA
    CCAGATTACGCAGAAAATGTGAAATTTGCAGATAACTGGCTAGCACCGAATCCTGGTTCA
    GATGCTGCAATTGCTCAAGCAATGACACATGTTATTTTACAAGAACATTATGTTAATCAA
    CCTAATGAACGCTTTATAAATTACGCTAAACAATATACAGATATGCCGTTTCTTATCATG
    CTGGATGAAGATGAAAATGGATATAAAGCGGGTCGATTTTTAAGAGCGAGTGACTTAGGT
    CAAACAACAGAGCAAGGCGAATGGAAGCCAGTTATTCATGATGCAATCAGCGATAGTTTA
    GTAGTACCTAATGGCACAATGGGTCAACGTTGGGAAGAAGGTAAGAAGTGGAACTTAAAA
    CTAGAAACAGAAGATGGTTCTAAAATTAACCCTACATTATCAATGGCAGAAGGTGGATAC
    GAATTAGAAACAATTCAATTCCCATACTTTGATAGTGATGGAGATGGGATATTCAATCGT
    CCAATTCCAACTCGACAAGTCACTTTAGCAAATGGTGACAAAGTCCGTATTGCTACAATT
    TTTGACTTAATGGCAAGTCAATATGGCGTGCGTCGTTTTGATCATAAATTAGAATCAAAA
    GGATACGACGATGCAGAATCAAAATATACACCTGCTTGGCAAGAAGCCATTTCAGGCGTA
    AAACAAAGTGTTGTCATTCAAGTAGCGAAAGAATTTGCGCAAAACGCTATCGATACTGAA
    GGGCGTTCAATGATTATCATGGGTGCGGGTATTAACCATTGGTTTAACTCAGATACGATT
    TATCGTTCAATCTTAAACTTAGTTATGTTATGTGGCTGTCAAGGTGTGAATGGTGGCGGT
    TGGGCTCACTATGTGGGACAAGAAAAATGTCGTCCGATTGAAGGATGGAGTACTGTCGCA
    TTTGCGAAAGACTGGCAAGGACCACCACGTTTGCAAAATGGAACAAGTTGGTTCTATTTT
    GCAACAGACCAATGGAAATATGAAGAGTCAAATGTAGATCGTTTAAAATCTCCATTAGCT
    AAAACAGAGGATTTAAAGCATCAACACCCAGCTGATTATAATGTTTTAGCAGCTAGACTT
    GGTTGGTTACCATCATATCCACAATTTAATAAAAATAGTTTGTTGTTTGCAGAAGAAGCT
    AAAGATGAAGGCATTGAGTCGAATGAGGCAATTTTAAAACGAGCGATAAATGAAGTTAAG
    TCAAAACAAACGCAATTTGCGATAGAAGATCCGGATTTGAAAAAGAATCATCCGAAATCA
    CTGTTTATATGGCGCTCAAATCTAATCTCAAGTTCTGCAAAAGGTCAAGAATACTTTATG
    AAGCATTTACTTGGCACAAAATCAGGGTTATTAGCTACACCAAATGAAGATGAAAAGCCA
    GAAGAAATTACGTGGCGTGAGGAAACAACAGGGAAATTAGATTTAGTCGTTTCTTTAGAT
    TTCAGAATGACAGCAACACCTTTATATTCTGACATTGTTTTGCCAGCAGCGACTTGGTAT
    GAGAAGCATGATTTGTCATCTACAGATATGCATCCATATGTACATCCTTTTAATCCAGCT
    ATTGATCCATTATGGGAATCGCGTTCAGACTGGGATATTTATAAAACGTTGGCAAAAGCA
    TTTTCAGAAATGGCAAAAGACTATTTACCTGGAACGTTTAAAGATGTTGTGACAACTCCA
    CTTAGTCATGATACAAAGCAAGAAATTTCAACACCATACGGCGTAGTGAAAGATTGGTCG
    AAGGGTGAAATTGAAGCGGTACCTGGACGTACAATGCCTAACTTTGCAATTGTAGAACGC
    GACTACACTAAAATTTACGACAAATATGTCACGCTTGGTCCTGTACTTGAAAAAGGGAAA
    GTTGGAGCACATGGTGTAAGTTTCGGTGTCAGTGAACAATATGAAGAATTAAAAAGTATG
    TTAGGTACGTGGAGTGATACAAATGATGATTCTGTGAGAGCGAATCGTCCGCGTATTGAT
    ACAGCACGTAATGTAGCAGATGCAATACTAAGTATTTCATCTGCTACGAATGGTAAATTA
    TCACAAAAATCATATGAAGATCTTGAAGAACAAACTGGAATGCCGTTAAAAGATATTTCT
    AGCGAACGTGCTGCTGAGAAAATTTCGTTTTTAAATATAACTTCACAACCACGAGAAGTA
    ATACCGACAGCAGTATTCCCAGGTTCAAATAAACAAGGTCGACGATATTCACCATTTACA
    ACGAATATAGAACGTCTAGTACCTTTTAGAACATTAACAGGACGTCAAAGTTATTATGTG
    GATCACGAAGTTTTCCAACAATTTGGGGAGAGCTTACCAGTATATAAACCGACATTGCCG
    CCAATGGTATTTGGGAATAGAGATAAGAAAATTAAAGGTGGTACAGATGCTTTGGTACTG
    CGTTATTTAACGCCTCATGGAAAATGGAATATACACTCAATGTATCAAGATAATAAGCAT
    ATGTTGACACTATTTAGAGGTGGTCCAACGGTTTGGATATCAAATGAAGATGCTGAAAAA
    CACGATATCCAAGATAATGATTGGCTAGAAGTGTATAACCGTAATGGTGTTGTAACGGCA
    AGAGCAGTTATTTCGCATCGTATGCCTAAAGGTACAATGTTTATGTATCATGCACAAGAT
    AAACATATTCAAACGCCTGGGTCAGAAATTACAGATACACGTGGTGGTTCACACAACGCG
    CCGACTAGAATCCATTTGAAACCAACACAACTAGTCGGAGGATACGCACAAATTAGTTAT
    CACTTTAATTATTATGGACCAATTGGGAACCAAAGGGATTTATATGTAGCAGTTAGAAAG
    ATGAAGGAGGTTAATTGGCTTGAAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
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    1320
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    1920
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    2160
    2220
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    2400
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    2520
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    2640
    2700
    2760
    2820
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    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3690

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2185 [new locus tag: SA_RS12535 ]
  • symbol: NarG
  • description: respiratory nitrate reductase subunit alpha
  • length: 1229
  • theoretical pI: 6.30843
  • theoretical MW: 139846
  • GRAVY: -0.589097

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismEnergy metabolismAnaerobicnitrate reductase, alpha subunit (TIGR01580; EC 1.7.99.4; HMM-score: 1838.6)
    DMSO reductase family type II enzyme, molybdopterin subunit (TIGR03479; HMM-score: 592.8)
    anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family (TIGR02166; HMM-score: 180.3)
    formate dehydrogenase, alpha subunit (TIGR01591; EC 1.2.1.2; HMM-score: 132.8)
    molybdopterin guanine dinucleotide-containing S/N-oxide reductases (TIGR00509; HMM-score: 103)
    trimethylamine-N-oxide reductase TorA (TIGR02164; EC 1.7.2.3; HMM-score: 78.7)
    MetabolismEnergy metabolismAnaerobicformate dehydrogenase-N alpha subunit (TIGR01553; EC 1.1.5.6; HMM-score: 46.9)
    MetabolismEnergy metabolismElectron transportformate dehydrogenase-N alpha subunit (TIGR01553; EC 1.1.5.6; HMM-score: 46.9)
    MetabolismEnergy metabolismElectron transportarsenite oxidase, large subunit (TIGR02693; EC 1.20.9.1; HMM-score: 28.2)
    MetabolismCentral intermediary metabolismNitrogen metabolismperiplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.7)
    MetabolismEnergy metabolismElectron transportperiplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.7)
    MetabolismEnergy metabolismAerobicperiplasmic nitrate reductase, large subunit (TIGR01706; EC 1.7.99.4; HMM-score: 24.7)
    oxidoreductase alpha (molybdopterin) subunit (TIGR01701; HMM-score: 19.7)
    MetabolismEnergy metabolismElectron transportNADH dehydrogenase (quinone), G subunit (TIGR01973; EC 1.6.99.5; HMM-score: 14)
  • TheSEED:  
    Nitrogen MetabolismNitrogen Metabolism - no subcategoryNitrate and nitrite ammonification Respiratory nitrate reductase alpha chain (EC 1.7.99.4) 
  • PFAM:
    no clan definedMolybdopterin; Molybdopterin oxidoreductase (PF00384; HMM-score: 539.6)
    AcetylDC-like (CL0332) Molydop_binding; Molydopterin dinucleotide binding domain (PF01568; HMM-score: 75.9)
    4Fe-4S (CL0344) Nitr_red_alph_N; Respiratory nitrate reductase alpha N-terminal (PF14710; HMM-score: 38.9)
    Molybdop_Fe4S4; Molybdopterin oxidoreductase Fe4S4 domain (PF04879; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.139
    • Ymax_pos: 11
    • Cmax: 0.109
    • Cmax_pos: 18
    • Smax: 0.239
    • Smax_pos: 1
    • Smean: 0.183
    • D: 0.156
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 15927975 NCBI
  • RefSeq: NP_375508 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVRTTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYSPLRIKYPYIRGKLWDLWTEALEENYGNRVAAWASIVENEDKAKQYKQARGMGGHVRSNWKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDWYADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKVISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTDMPFLIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWEEGKKWNLKLETEDGSKINPTLSMAEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLANGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQVAKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVGQEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEDLKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIESNEAILKRAINEVKSKQTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKPEEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFNPAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVVKDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQYEELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQTGMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFRTLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGKWNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISHRMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNYYGPIGNQRDLYVAVRKMKEVNWLED

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: SA1851 (Rex*), SA2179 (NreC*)
    Rex* (TF) important in Energy metabolismRegPrecise
    NreC* (TF) important in Nitrate and nitrite respirationRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]