From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2226 [new locus tag: SA_RS12780 ]
  • pan locus tag?: SAUPAN005986000
  • symbol: SA2226
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2226 [new locus tag: SA_RS12780 ]
  • symbol: SA2226
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2498051..2499091
  • length: 1041
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAAGAAAATTGCATAGAGAGTTGAATAACAGACACATCCAATTGATAGCAATTGGG
    GGCGCAATTGGAACTGGGTTATTCCTAGGATCAGGTCAAACAATATCTTTAACTGGTCCA
    TCACTGTTATTCACATACATGATTATTGGGGTTGTACTATTCGCTTTTATGCGCGCATTA
    GGCGAATTGTTGTTGAGCAATACAAGATTTAATTCATTTGTTGATATTGCAAATGAATAT
    TTAGGCCCTTTTGGTGGCTTTGTCATTGGCTGGACTTACTGGTTATGTTGGATTGTATCA
    AGTATGTCAGACCTAACTGCGATGGGACAATACTTTGCATTTTGGTATCCACAAGTCCCA
    AATTGGATTACCGTGCTATTTATTGTTTTAATCTTGATTAGCTTCAACTTATTAGGTGCC
    AGATTATTTGGTGAACTGGAGTTTTGGTTCTCTATTATTAAAGTTGTCACAATTATTGCG
    ATGGTTATCGTTGGTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCA
    TCATTCACAAACTTAATCAGTCACGGCGGCATGTTCCCTGGAGGAACATTTGGTTTCCTA
    ATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCT
    GGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGT
    ATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATACCTTGGAATGAT
    ATCGATCCAAATAGTAGCCCTTTCGTTAAACTCTTTACATTAATCGGCGTGCCATTTGCA
    GCAGGCGTCGTTAACTTTGTCGTGCTAACTGCCGCGGCCTCTGCTACAAATAGTGGTATC
    TATTCGAATAGTCGTATCTTATTCGGACTGTCACAACAAGGGTTAGGTCCTAAAGTTTTA
    AATTGGCGTGCCTTATTTATCAATGTTAGTTTCATCAATTGCATTACTTATAGCAGCCTT
    GTTAAACTACATTTTCCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1041

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2226 [new locus tag: SA_RS12780 ]
  • symbol: SA2226
  • description: hypothetical protein
  • length: 346
  • theoretical pI: 9.47179
  • theoretical MW: 38143.9
  • GRAVY: 0.815029

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesGABA permease (TIGR01773; HMM-score: 274)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid permease (yeast) (TIGR00913; HMM-score: 236.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid transporter (TIGR00909; HMM-score: 105.2)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesethanolamine permease (TIGR00908; HMM-score: 68.3)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminescationic amino acid transport permease (TIGR00906; HMM-score: 62.3)
    histidine-histamine antiporter (TIGR04298; HMM-score: 52.9)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization D-serine/D-alanine/glycine transporter 
    CarbohydratesCentral carbohydrate metabolismPyruvate Alanine Serine Interconversions D-serine/D-alanine/glycine transporter 
  • PFAM:
    APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 282.1)
    AA_permease_2; Amino acid permease (PF13520; HMM-score: 118.7)
    Spore_permease; Spore germination protein (PF03845; HMM-score: 23.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.13
    • Ymax_pos: 25
    • Cmax: 0.115
    • Cmax_pos: 37
    • Smax: 0.221
    • Smax_pos: 16
    • Smean: 0.159
    • D: 0.141
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit source | edit]

  • GI: 15928016 NCBI
  • RefSeq: NP_375549 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLFTYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSMSDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIAMVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGVTAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIGVPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNWRALFINVSFINCITYSSLVKLHFP

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CcpA, CodY
    CcpA (TF) important in Carbon catabolism  RegPrecise
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]