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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2291 [new locus tag: SA_RS13135 ]
  • pan locus tag?: SAUPAN006098000
  • symbol: fnb
  • pan gene symbol?: fnbA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2291 [new locus tag: SA_RS13135 ]
  • symbol: fnb
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2571889..2575005
  • length: 3117
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    GTGAAAAACAATCTTAGGTACGGCATTAGAAAACATAAATTGGGAGCAGCATCAGTATTC
    TTAGGAACAATGATCGTTGTTGGGATGGGACAAGATAAAGAAGCTGCAGCATCAGAACAA
    AAGACAACTACAGTAGAAGAAAATGGGAATTCAGCTACTGATAATAAAACAAGTGAAACA
    CAAACAACTGCTACTAACGTTAATCATATAGAAGAAACTCAATCATATAACGCAACAGTA
    ACAGAACAACCGTCAAACGCAACACAAGTAACAACTGAAGAAGCACCAAAAGCAGTACAA
    GCACCACAAACTGCACAACCAGCAAATGTAGAAACAGTTAAAGAAGAAGAGAAACCTCAA
    GTTAAGGAAACGACACAACCTCAAGACAATAGCGGAAATCAAAGACAAGTAGATTTAACA
    CCTAAAAAGGTTACACAAAATCAAGGGACAGAAACACAAGTTGAAGTGGCACAGCCAAGA
    ACGGCATCAGAAAGTAAGCCACGTGTGACAAGATCAGCAGATGTAGCGGAAGCTAAGGAA
    GCTAGTGACGTTTCAGAAGTTAAAGGCACAGATGTTACAAGTAAAGTTACAGTAGAAAGT
    GGTTCTATTGAGGCACCTCAAGGAAATAAAGTAGAGCCACATGCTGGTCAACGTGTCGTA
    TTGAAATACAAATTGAAATTCGCAGATGGATTAAAAAGAGGAGATTATTTTGATTTTACA
    TTATCAAATAATGTAAATACTTATGGGGTTTCAACAGCTAGAAAGGTACCAGAGATTAAA
    AATGGCTCAGTTGTAATGGCTACAGGTGAGATCTTAGGGAATGGTAACATAAGATATACA
    TTTACTAACGAAATTGAACACAAGGTAGAGGTAACAGCTAATTTAGAAATCAACTTATTT
    ATTGACCCTAAAACTGTACAAAGCAATGGAGAACAAAAGATTACTTCTAAATTAAATGGT
    GAAGAAACAGAAAAAACAATACCAGTTGTTTATAATCCAGGTGTTAGCAATAGTTATACA
    AATGTAAATGGATCAATTGAAACATTTAATAAAGAATCTAATAAATTTACACATATAGCT
    TATATTAAGCCAATGAATGGAAACCAGTCAAACACTGTATCAGTAACAGGGACGTTGACT
    GAAGGTAGTAATTTAGCTGGTGGACAACCTACTGTTAAAGTATATGAATATCTAGGGAAA
    AAAGATGAATTGCCACAAAGTGTTTATGCAAATACATCAGATACTAACAAATTCAAAGAT
    GTAACAAAGGAAATGAATGGAAAATTGAGTGTGCAAGACAATGGTAGTTACTCATTGAAT
    TTAGATAAGTTGGATAAAACGTATGTCATTCATTATACAGGTGAATATTTGCAAGGGTCA
    GATCAGGTTAATTTTAGAACTGAATTATATGGGTATCCAGAACGAGCATATAAATCTTAC
    TATGTTTATGGGGGATATCGTTTAACTTGGGATAATGGTTTAGTTTTATATAGCAATAAA
    GCTGACGGCAATGGTAAAAATGGACAAATTATTCAAGATAATGATTTTGAATATAAAGAA
    GATACTGCAAAAGGAACTATGAGCGGGCAGTACGATGCCAAGCAAATTATTGAAACAGAA
    GAAAATCAAGACAATACACCGCTTGACATTGATTACCACACAGCTATAGATGGTGAGGGT
    GGTTATGTTGATGGGTATATTGAAACAATAGAAGAAACGGATTCATCAGCTATTGATATC
    GATTACCATACTGCTGTGGATAGTGAAGTGGGTCACGTTGGAGGATACACTGAGTCCTCT
    GAGGAATCAAATCCAATTGACTTTGAAGAATCGACACATGAAAATTCAAAACATCACGCT
    GATGTTGTTGAATATGAAGAGGATACAAATCCAGGTGGTGGCCAAGTAACAACTGAGTCT
    AACTTAGTTGAATTTGACGAAGAGTCTACAAAAGGTATTGTAACTGGCGCAGTGAGCGAC
    CATACAACAATTGAAGATACGAAAGAATATACGACTGAAAGTAATCTGATTGAACTAGTA
    GATGAACTACCTGAAGAACATGGTCAAGCACAAGGACCAATCGAGGAAATTACTGAAAAC
    AATCATCATATTTCTCATTCTGGTTTAGGAACTGAAAATGGTCACGGTAATTATGGCGTG
    ATTGAAGAAATCGAAGAAAATAGCCACGTTGATATTAAGAGTGAATTAGGTTACGAAGGT
    GGCCAAAATAGCGGTAACCAGTCATTCGAGGAAGACACAGAAGAAGACAAACCTAAATAT
    GAACAAGGTGGCAATATCGTAGATATCGATTTCGACAGTGTACCTCAAATTCATGGTCAA
    AATAAAGGTGACCAGTCATTCGAAGAAGATACAGAGAAAGACAAGCCTAAATATGAACAT
    GGCGGTAATATCATTGATATCGACTTCGACAGTGTGCCACAAATTCATGGATTCAATAAG
    CATAATGAAATTATTGAAGAAGATACAAACAAAGATAAACCTAATTATCAATTCGGTGGA
    CACAATAGTGTTGACTTTGAAGAAGATACACTTCCAAAAGTAAGCGGCCAAAATGAAGGT
    CAACAAACGATTGAAGAAGATACAACGCCGCCAACGCCACCGACACCAGAAGTACCGAGT
    GAGCCGGAAACACCAATGCCACCGACACCAGAAGTACCGAGTGAGCCGGAAACACCAACG
    CCACCAACACCAGAGGTACCAAGTGAGCCGGAAACACCAACACCACCGACTCCGGAAGTA
    CCAAGTGAGCCGGAAACACCAACACCACCGACACCAGAAGTGCCGAGTGAGCCAGAAACA
    CCAACACCGCCAACACCAGAGGTACCAGCTGAACCTGGTAAACCAGTACCACCCGCAAAA
    GAAGAACCTAAAAAGCCTTCTAAACCAGTGGAACAAGGTAAAGTAGTAACACCTGTTATT
    GAAATCAATGAAAAGGTTAAAGCAGTGGCACCAACTAAAAAAGCACAATCTAAGAAATCT
    GAACTACCTGAAACAGGTGGAGAAGAATCAACAAACAAAGGTATGTTGTTCGGCGGATTA
    TTCAGCATTCTAGGTTTAGCATTATTACGCAGAAATAAAAAGAATAACAAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3117

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2291 [new locus tag: SA_RS13135 ]
  • symbol: Fnb
  • description: hypothetical protein
  • length: 1038
  • theoretical pI: 4.26899
  • theoretical MW: 113618
  • GRAVY: -0.943545

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 25.1)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 21.9)
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Fibronectin binding protein FnbA 
  • PFAM:
    Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 115.7)
    no clan definedFn_bind; Fibronectin binding repeat (PF02986; HMM-score: 104.5)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 33)
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 32.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :LPETG
  • SignalP: Signal peptide length 37 aa
    • Ymax: 0.635
    • Ymax_pos: 37
    • Cmax: 0.493
    • Cmax_pos: 37
    • Smax: 0.88
    • Smax_pos: 34
    • Smean: 0.8
    • D: 0.699
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTTVEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAPQTAQPANVETVKEEEKPQVKETTQPQDNSGNQRQVDLTPKKVTQNQGTETQVEVAQPRTASESKPRVTRSADVAEAKEASDVSEVKGTDVTSKVTVESGSIEAPQGNKVEPHAGQRVVLKYKLKFADGLKRGDYFDFTLSNNVNTYGVSTARKVPEIKNGSVVMATGEILGNGNIRYTFTNEIEHKVEVTANLEINLFIDPKTVQSNGEQKITSKLNGEETEKTIPVVYNPGVSNSYTNVNGSIETFNKESNKFTHIAYIKPMNGNQSNTVSVTGTLTEGSNLAGGQPTVKVYEYLGKKDELPQSVYANTSDTNKFKDVTKEMNGKLSVQDNGSYSLNLDKLDKTYVIHYTGEYLQGSDQVNFRTELYGYPERAYKSYYVYGGYRLTWDNGLVLYSNKADGNGKNGQIIQDNDFEYKEDTAKGTMSGQYDAKQIIETEENQDNTPLDIDYHTAIDGEGGYVDGYIETIEETDSSAIDIDYHTAVDSEVGHVGGYTESSEESNPIDFEESTHENSKHHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDTEKDKPKYEHGGNIIDIDFDSVPQIHGFNKHNEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPETPMPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKAQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNNKA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

T J Foster, M Höök
Surface protein adhesins of Staphylococcus aureus.
Trends Microbiol.: 1998, 6(12);484-8
[PubMed:10036727] [WorldCat.org] (P p)