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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2426 [new locus tag: SA_RS13910 ]
  • pan locus tag?: SAUPAN006362000
  • symbol: arcD
  • pan gene symbol?: arcD
  • synonym:
  • product: arginine/oirnithine antiporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2426 [new locus tag: SA_RS13910 ]
  • symbol: arcD
  • product: arginine/oirnithine antiporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2723039..2724469
  • length: 1431
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAACGAATCAGGAGATAACAAACTCAGTAAATCTTCTTTAATTGGACTAGTTATAGGA
    TCCATGATTGGTGGCGGTGCGTTCAATATAATGTCTGATATGGGCGGTAAAGCCGGTGGA
    TTAGCCATTATTATTGGTTGGATTATTACAGCTATAGGAATGATTTCATTAGCGTTCGTA
    TTTCAAAATTTAACCAATGAACGGCCGGAGCTAGACGGTGGTATTTATAGTTATGCTCAA
    GCAGGATTTGGCGATTTTGTAGGATTTATCAGTGCTTGGGGATATTGGTTCTCAGCGTTT
    TTAGGCAATGTTGCCTATGCAACACTATTGATGTCAGCAGTAGGTAACTTTTTCCCGATT
    TTTAAAGGAGGCAACACATTACCAAGTATTATTGTCGCCTCGTTACTACTCTGGGGTGTC
    CATTTCTTGATTTTAAAAGGCGTTGAAACAGCAGCATTTATCAATAGTATTGTTACTGTT
    GCAAAGTTAATCCCGATTTTACTTGTAATCATATGCATGATAATTGCATTCAATTTTGAC
    ACTTTTAAAACAGGCTTTTTCGGTATGACGTCAGAGGGTGTATTGCCATTTAGTTGGGCG
    AGCACAATGAGCCAAGTTAAAAGTACGATGCTAGTGACAGTTTGGGTGTTTATCGGTATC
    GAAGGTGCAGTAATTTTTTCTAGTAGAGCTAAAAATAAAAAAGATGTAGGTAGTGCCACG
    GTTATAGGACTTATATCAGTTTTAATTATCTATTTCTTATTAACTGTATTAGCTCAAGGC
    GTGATTTTGCAAAATCATATTTCTCAATTAGATTCGCCAAGTATGGCACAGGTGCTTGCA
    ACTATTGTAGGTGGTTGGGGATCTACACTTGTAAATATTGGTTTAATTATTTCGGTACTA
    GGTGCATGGTTAGGATGGACATTGCTTGCTGGTGAATTACCTTTCATTGTTGCAAAAGAT
    GGATTATTTCCAAAGTGGTTTGCTAAAGAAAATAAAAATGGAGCACCTGTAAATGCACTG
    CTTATTACCAATATATTAGTACAATTATTTTTAATAAGTATGCTATTTACACAGAGTGCG
    TATCAATTTGCATTTTCACTAGCATCAAGTGCTATTTTATACCCTTACATGTTCAGTGCA
    TTTTACCAAGTTAAATACACTTTAGAGCATCGACAGCAAGCAACTACTAAACAATGGACG
    ATTGGCATCATAGCCTCAATTTATGCTATATGGCTTATATATGCAGCAGGTATCAATTAC
    TTATTATTGACGATGTTACTTTATATTCCAGCTCTTCTTGTTTATTCAATCGTTCAAAAG
    AATAATCAGACACGTTTGATTAAATCAGACTATATTCTTTTTATGATTATTATCGTACTT
    GCAGTTATCGGATTAATTAAGTTATTGATGGGAACGATAAATGTTTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1431

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2426 [new locus tag: SA_RS13910 ]
  • symbol: ArcD
  • description: arginine/oirnithine antiporter
  • length: 476
  • theoretical pI: 9.50412
  • theoretical MW: 51686.1
  • GRAVY: 0.909244

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesarginine-ornithine antiporter (TIGR03810; HMM-score: 621.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminestransporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 571.4)
    histidine-histamine antiporter (TIGR04298; HMM-score: 139.4)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid transporter (TIGR00909; HMM-score: 98.1)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 80.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminescationic amino acid transport permease (TIGR00906; HMM-score: 57.5)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid permease (yeast) (TIGR00913; HMM-score: 53.9)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 46.9)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesglutamate:gamma-aminobutyrate antiporter (TIGR00910; HMM-score: 24.1)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesspore germination protein (amino acid permease) (TIGR00912; HMM-score: 21.7)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine and Ornithine Degradation Arginine/ornithine antiporter ArcD 
    Arginine; urea cycle, polyaminesArginine Deiminase Pathway Arginine/ornithine antiporter ArcD 
    Arginine; urea cycle, polyaminesPolyamine Metabolism Arginine/ornithine antiporter ArcD 
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 241.5)
    AA_permease; Amino acid permease (PF00324; HMM-score: 83.4)
    Spore_permease; Spore germination protein (PF03845; HMM-score: 30.4)
    no clan definedYwiC; YwiC-like protein (PF14256; HMM-score: 17.3)
    Gemini_mov; Geminivirus putative movement protein (PF01708; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 13
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.176
    • Ymax_pos: 33
    • Cmax: 0.131
    • Cmax_pos: 37
    • Smax: 0.401
    • Smax_pos: 24
    • Smean: 0.221
    • D: 0.194
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

  • GI: 15928219 NCBI
  • RefSeq: NP_375752 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAIIIGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLGNVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTVAKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFIGIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMAQVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNGAPVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQQATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSDYILFMIIIVLAVIGLIKLLMGTINVF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: SA1851 (Rex*), SA2424 (ArcR*), AhrC (ArgR*), CcpA
    Rex* (TF) important in Energy metabolism  RegPrecise
    ArcR* (TF) important in Arginine degradation  RegPrecise
    AhrC  (ArgR*) (TF) important in Arginine biosynthesis, Arginine degradation  RegPrecise
    CcpA (TF) important in Carbon catabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]