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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2568 [new locus tag: SAUSA300_RS14285 ]
  • pan locus tag?: SAUPAN006362000
  • symbol: arcD
  • pan gene symbol?: arcD
  • synonym:
  • product: arginine/ornithine antiporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_2568 [new locus tag: SAUSA300_RS14285 ]
  • symbol: arcD
  • product: arginine/ornithine antiporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2779150..2780580
  • length: 1431
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAACGAATCAGGAGATAACAAACTCAGTAAATCTTCTTTAATTGGACTAGTTATAGGA
    TCCATGATTGGTGGCGGTGCGTTCAATATAATGTCTGATATGGGCGGTAAAGCCGGTGGA
    TTAGCCATTATTATTGGTTGGATTATTACAGCTATAGGAATGATTTCATTAGCGTTCGTA
    TTTCAAAATTTAACCAATGAACGGCCGGAGCTAGACGGTGGTATTTATAGTTATGCTCAA
    GCAGGATTTGGCGATTTTGTAGGATTTATCAGTGCTTGGGGATATTGGTTCTCAGCGTTT
    TTAGGCAATGTTGCCTATGCAACACTATTGATGTCAGCAGTAGGTAACTTTTTCCCGATT
    TTTAAAGGAGGCAACACATTACCAAGTGTTATTGTCGCCTCGTTACTACTCTGGGGTGTC
    CATTTCTTGATTTTAAAAGGCGTTGAAACAGCAGCATTTATCAATAGTATTGTTACTGTT
    GCAAAGTTAATACCGATTTTACTTGTAATCATATGCATGATAATTGCATTCAATTTTGAC
    ACTTTTAAAACAGGCTTTTTCAGTATGACGTCAGAGGGTGTATTGCCATTTAGTTGGGCG
    AGCACAATGAGCCAAGTTAAAAGTACGATGCTAGTGACAGTTTGGGTGTTTATCGGTATC
    GAAGGTGCAGTAATTTTTTCTAGTAGAGCTAAAAATAAAAAAGATGTAGGTAGTGCCACG
    GTTATAGGACTTATATCAGTTTTAATTATCTATTTCTTATTAACTGTATTAGCTCAAGGC
    GTGATTTTGCAAAATCATATTTCGCAATTAGATTCGCCAAGTATGGCACAGGTGCTTGCA
    ACTATTGTAGGTGGTTGGGGATCTACACTTGTAAATATTGGTTTAATTATTTCGGTACTA
    GGTGCATGGTTAGGATGGACACTGCTTGCTGGTGAATTACCTTTCATTGTTGCAAAAGAT
    GGATTATTTCCAAAATGGTTTGCTAAAGAAAATAAAAATGGAGCACCTGTAAATGCACTG
    CTTATTACCAATATATTAGTACAATTATTTTTAATAAGTATGCTATTTACACAGAGTGCG
    TATCAATTTGCATTTTCACTAGCATCAAGTGCTATTTTATACCCTTACATGTTCAGTGCA
    TTTTACCAAGTTAAATACACTTTAGAGCATCGACAGCAAGCAACTACTAAACAATGGACG
    ATTGGTATCATAGCCTCAATTTATGCTATATGGCTTATATATGCAGCAGGTATCAATTAC
    TTATTATTGACGATGTTACTTTATATTCCAGCTCTTCTTGTTTATACAATCGTTCAAAAG
    AATAATCAGACACGTTTGATTAAATCAGACTATATTCTTTTTATGATTATTATCGTACTT
    GCAGTTATCGGGTTAATTAAGTTATTGATGGGAACGATAAATGTTTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1431

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_2568 [new locus tag: SAUSA300_RS14285 ]
  • symbol: ArcD
  • description: arginine/ornithine antiporter
  • length: 476
  • theoretical pI: 9.50412
  • theoretical MW: 51716.2
  • GRAVY: 0.907983

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesarginine-ornithine antiporter (TIGR03810; HMM-score: 619.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminestransporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 568.9)
    histidine-histamine antiporter (TIGR04298; HMM-score: 140)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid transporter (TIGR00909; HMM-score: 99)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 81.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminescationic amino acid transport permease (TIGR00906; HMM-score: 59.2)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesamino acid permease (yeast) (TIGR00913; HMM-score: 51.9)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 46.1)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesglutamate:gamma-aminobutyrate antiporter (TIGR00910; HMM-score: 24.4)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesspore germination protein (amino acid permease) (TIGR00912; HMM-score: 21.5)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine and Ornithine Degradation Arginine/ornithine antiporter ArcD 
    Arginine; urea cycle, polyaminesArginine Deiminase Pathway Arginine/ornithine antiporter ArcD 
    Arginine; urea cycle, polyaminesPolyamine Metabolism Arginine/ornithine antiporter ArcD 
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 240.8)
    AA_permease; Amino acid permease (PF00324; HMM-score: 84.2)
    Spore_permease; Spore germination protein (PF03845; HMM-score: 30.4)
    no clan definedYwiC; YwiC-like protein (PF14256; HMM-score: 17.9)
    Gemini_mov; Geminivirus putative movement protein (PF01708; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 13
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.176
    • Ymax_pos: 33
    • Cmax: 0.131
    • Cmax_pos: 37
    • Smax: 0.401
    • Smax_pos: 24
    • Smean: 0.221
    • D: 0.194
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAIIIGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLGNVAYATLLMSAVGNFFPIFKGGNTLPSVIVASLLLWGVHFLILKGVETAAFINSIVTVAKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFIGIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMAQVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNGAPVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQQATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSDYILFMIIIVLAVIGLIKLLMGTINVF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: ArcR, ArgR, CcpA, Rex
    ArcR (TF) important in Arginine degradation  RegPrecise    transcription unit transferred from N315 data RegPrecise
    ArgR (TF) important in Arginine biosynthesis, Arginine degradation  RegPrecise    transcription unit transferred from N315 data RegPrecise
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise
    Rex (TF) important in Energy metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]