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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2569 [new locus tag: SAUSA300_RS14290 ]
  • pan locus tag?: SAUPAN006363000
  • symbol: arcB
  • pan gene symbol?: arcB
  • synonym:
  • product: ornithine carbamoyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_2569 [new locus tag: SAUSA300_RS14290 ]
  • symbol: arcB
  • product: ornithine carbamoyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2780671..2781681
  • length: 1011
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAGAAATTCAAAAACCGTATGATTTAAAAGGCAGATCATTATTAAAAGAAAGTGAT
    TTTACCAAAGCAGAATTCGAAGGACTTATTGATTTTGCAATTACATTAAAAGAGTATAAG
    AAAAACGGTATTAAGCATCACTACTTATCTGGAAAAAATATTGCACTACTATTCGAAAAG
    AATTCGACGAGAACGCGTGCTGCGTTTACAGTTGCGTCTATTGATTTAGGTGCGCATCCA
    GAATTTTTAGGAAAAAATGATATTCAATTAGGCAAAAAAGAATCTGTAGAGGATACTGCG
    AAAGTATTAGGTAGAATGTTCGATGGTATTGAATTCCGTGGTTTTTCACAACAAGCTGTT
    GAAGATTTAGCGAAGTTCTCTGGTGTACCGGTGTGGAATGGATTAACAGACGATTGGCAT
    CCTACACAAATGTTAGCTGATTTTATGACAATAAAAGAGAATTTTGGATATCTAGAAGGA
    ATAAACTTAACTTACGTTGGAGATGGACGTAATAATATTGCGCATTCATTAATGGTAGCA
    GGTGCTATGTTAGGTGTTAATGTAAGAATTTGTACACCTAAATCATTAAATCCAAAAGAG
    GCATATGTTGATATTGCAAAAGAAAAAGCGAGTCAATATGGTGGTTCAGTCATGATTACG
    GATAATATTGCAGAAGCAGTTGAAAATACAGATGCTATATATACAGATGTTTGGGTATCG
    ATGGGTGAAGAAAGTGAATTTGAACAACGTATTAATTTATTAAAAGACTATCAAGTGAAT
    CAACAGATGTTTGATTTAACAGGTAAAGATTCAACGATATTCTTACATTGTTTACCAGCA
    TTCCATGATACAAATACACTTTATGGACAAGAAATTTATGAAAAATATGGATTAGCTGAA
    ATGGAAGTTACAGACCAAATCTTTAGAAGTGAACATTCAAAAGTGTTTGATCAAGCTGAA
    AATAGAATGCATACAATTAAGGCAGTAATGGCAGCAACATTGGGGAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1011

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_2569 [new locus tag: SAUSA300_RS14290 ]
  • symbol: ArcB
  • description: ornithine carbamoyltransferase
  • length: 336
  • theoretical pI: 4.94437
  • theoretical MW: 37762.5
  • GRAVY: -0.331548

Function[edit source | edit]

  • reaction:
    EC 2.1.3.3?  ExPASy
    Ornithine carbamoyltransferaseCarbamoyl phosphate + L-ornithine = phosphate + L-citrulline
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyornithine carbamoyltransferase (TIGR00658; EC 2.1.3.3; HMM-score: 414)
    MetabolismEnergy metabolismAmino acids and aminesputrescine carbamoyltransferase (TIGR04384; EC 2.1.3.6; HMM-score: 261.7)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisaspartate carbamoyltransferase (TIGR00670; EC 2.1.3.2; HMM-score: 125.5)
    knotted carbamoyltransferase YgeW (TIGR03316; EC 2.1.3.-; HMM-score: 70.9)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesArginine and Ornithine Degradation Ornithine carbamoyltransferase (EC 2.1.3.3) 
    Arginine; urea cycle, polyaminesArginine Biosynthesis extended Ornithine carbamoyltransferase (EC 2.1.3.3) 
    Arginine; urea cycle, polyaminesArginine Deiminase Pathway Ornithine carbamoyltransferase (EC 2.1.3.3) 
  • PFAM:
    Asp-glut_race (CL0399) OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain (PF00185; HMM-score: 196.1)
    no clan definedOTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain (PF02729; HMM-score: 153.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAUSA300_0225putative acyl-CoA acetyltransferase FadA  [1] (data from MRSA252)
    SAUSA300_02263-hydroxyacyl-CoA dehydrogenase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.126
    • Ymax_pos: 11
    • Cmax: 0.136
    • Cmax_pos: 68
    • Smax: 0.222
    • Smax_pos: 2
    • Smean: 0.147
    • D: 0.134
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGIKHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKVLGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEGINLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVMITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLHCLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATLGS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: ArcR, ArgR, CcpA, Rex
    ArcR (TF) important in Arginine degradation  RegPrecise    transcription unit transferred from N315 data RegPrecise
    ArgR (TF) important in Arginine biosynthesis, Arginine degradation  RegPrecise    transcription unit transferred from N315 data RegPrecise
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise
    Rex (TF) important in Energy metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]