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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0097 [new locus tag: SACOL_RS00480 ]
  • pan locus tag?: SAUPAN000911000
  • symbol: sirC
  • pan gene symbol?: sirC
  • synonym:
  • product: iron compound ABC transporter permease SirC

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL0097 [new locus tag: SACOL_RS00480 ]
  • symbol: sirC
  • product: iron compound ABC transporter permease SirC
  • replicon: chromosome
  • strand: -
  • coordinates: 110432..111430
  • length: 999
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACCGAAAAGATTAATAAAAAAGACAATTACCATCTCATCTTCGCGTTAATCTTTTTA
    GCCATCGTTTCAGTGGTAAGTATGATGATTGGTTCAAGCTTTATACCATTACAACGCGTA
    CTGATGTACTTTATAAATCCAAATGACAGTATGGATCAATTCACTTTAGAAGTATTACGC
    TTACCTCGCATTACACTTGCGATTTTAGCAGGTGCCGCACTAGGAATGAGTGGTTTAATG
    TTGCAAAATGTATTAAAAAATCCAATTGCCTCACCTGATATTATCGGTATCACAGGTGGT
    GCTAGCTTAAGTGCTGTTGTCTTTATTGCATTTTTCAGCCATTTAACAATACATTTACTT
    CCACTATTTGCAGTATTAGGTGGCGCAGTTGCAATGATGATACTATTAGTGTTTCAAACG
    AAAGGACAAATACGCCCGACAACACTCATAATCATCGGTATTTCGATGCAAACGTTGTTT
    ATTGCGCTTGTCCAAGGATTACTCATTACAACGAAGCAATTATCTGCTGCCAAAGCTTAT
    ACATGGCTAGTCGGAAGTCTTTACGGTGCTACGTTTAAAGATACAATCATTTTGGGTATG
    GTTATTTTAGCTGTTGTGCCGTTGTTATTTCTTGTTATACCAAAAATGAAAATATCTATA
    CTTGATGACCCTGTAGCGATTGGCTTAGGCTTACATGTACAACGTATGAAACTAATCCAA
    TTAATCACTTCTACTATACTCGTATCTATGGCAATCAGTTTAGTAGGTAACATTGGGTTT
    GTCGGTTTAATCGCACCACATATCGCGAAAACAATCGTTCGCGGAAGTTATGCTAAAAAG
    TTACTAATGTCAGCAATGATTGGTGCCATATCAATTGTTATTGCAGACTTAATTGGGCGT
    ACCTTATTCTTGCCTAAAGAAGTGCCAGCAGGTGTATTTATTGCTGCTTTTGGTGCCCCA
    TTCTTCATATACTTATTATTAACCGTGAAAAAGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL0097 [new locus tag: SACOL_RS00480 ]
  • symbol: SirC
  • description: iron compound ABC transporter permease SirC
  • length: 332
  • theoretical pI: 10.6652
  • theoretical MW: 35729.5
  • GRAVY: 1.11657

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 149.6)
    MetabolismTransport and binding proteinsUnknown substrateanchored repeat-type ABC transporter, permease subunit (TIGR03770; HMM-score: 27.5)
  • TheSEED:  
    Iron acquisition and metabolismSiderophoresSiderophore Staphylobactin Siderophore staphylobactin ABC transporter, permease protein SirC 
  • PFAM:
    Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 278.5)
    ABC-3; ABC 3 transport family (PF00950; HMM-score: 18.3)
    ISOCOT_Fold (CL0246) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.169
    • Ymax_pos: 43
    • Cmax: 0.177
    • Cmax_pos: 32
    • Smax: 0.313
    • Smax_pos: 36
    • Smean: 0.154
    • D: 0.163
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYFINPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGASLSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLFIALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKISILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGSYAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SACOL1541 (Fur*)
    Fur* (TF) important in Iron homeostasis  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J. Bacteriol.: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)