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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1140 [new locus tag: SACOL_RS05820 ]
  • pan locus tag?: SAUPAN003359000
  • symbol: isdA
  • pan gene symbol?: isdA
  • synonym:
  • product: LPXTG cell wall surface anchor protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1140 [new locus tag: SACOL_RS05820 ]
  • symbol: isdA
  • product: LPXTG cell wall surface anchor protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1148029..1149081
  • length: 1053
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACAAAACATTATTTAAACAGTAAGTATCAATCAGAACAACGTTCATCAGCTATGAAA
    AAGATTACAATGGGTACAGCATCTATCATTTTAGGTTCCCTTGTATACATAGGCGCAGAC
    AGCCAACAAGTCAATGCGGCAACAGAAGCTACGAACGCACCTAATAATCAAAGCACACAA
    GTTTCTCAAGCAACATCACAACCAATTAATTTCCAAGTGCAAAAAGATGGCTCTTCTGAG
    AAGTCACACATGGATGACTATATGCAACACCCTGGTAAAGTAATTAAACAAAATAATAAA
    TATTATTTCCAAACCGTGTTAAACAATGCATCATTCTGGAAAGAATACAAATTTTACAAT
    GCAAACAATCAAGAATTAGCAACAACTGTTGTTAACGATAATAAAAAAGCGGATACTAGA
    ACAATCAATGTTGCAGTTGAACCTGGATATAAGAGCTTAACTACTAAAGTACATATTGTC
    GTGCCACAAATTAATTACAATCATAGATATACTACGCATTTGGAATTTGAAAAAGCAATT
    CCTACATTAGCTGACGCAGCAAAACCAAACAATGTTAAACCGGTTCAACCAAAACCAGCT
    CAACCTAAAACACCTACTGAGCAAACTAAACCAGTTCAACCTAAAGTTGAAAAAGTTAAA
    CCTACTGTAACTACAACAAGCAAAGTTGAAGACAATCACTCTACTAAAGTTGTAAGTACT
    GACACAACAAAAGATCAAACTAAAACACAAACTGCTCATACAGTTAAAACAGCACAAACT
    GCTCAAGAACAAAATAAAGTTCAAACACCTGTTAAAGATGTTGCAACAGCGAAATCTGAA
    AGCAACAATCAAGCTGTAAGTGATAATAAATCACAACAAACTAACAAAGTTACAAAACAT
    AACGAAACGCCTAAACAAGCATCTAAAGCTAAAGAATTACCAAAAACTGGTTTAACTTCA
    GTTGATAACTTTATTAGCACAGTTGCCTTCGCAACACTTGCCCTTTTAGGTTCATTATCT
    TTATTACTTTTCAAAAGAAAAGAATCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1053

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1140 [new locus tag: SACOL_RS05820 ]
  • symbol: IsdA
  • description: LPXTG cell wall surface anchor protein
  • length: 350
  • theoretical pI: 10.2642
  • theoretical MW: 38741.2
  • GRAVY: -0.820286

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    heme uptake protein IsdC (TIGR03656; HMM-score: 59.6)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 22.5)
    pullulanase, extracellular (TIGR02102; HMM-score: 16)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 15.9)
  • TheSEED:  
    Cell Wall and CapsuleGram-Positive cell wall componentsSortase Cell surface protein IsdA, transfers heme from hemoglobin to apo-IsdC 
    Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryHeme, hemin uptake and utilization systems in GramPositives Cell surface protein IsdA, transfers heme from hemoglobin to apo-IsdC 
  • PFAM:
    no clan definedNEAT; Iron Transport-associated domain (PF05031; HMM-score: 87.5)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 37.1)
    DUF5044; Domain of unknown function (DUF5044) (PF16447; HMM-score: 15.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :LPKTG
  • SignalP: Signal peptide length 47 aa
    • Ymax: 0.61
    • Ymax_pos: 47
    • Cmax: 0.503
    • Cmax_pos: 47
    • Smax: 0.841
    • Smax_pos: 37
    • Smean: 0.589
    • D: 0.602
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQVNAATEATNAPNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYYFQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIVVPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEKVKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVATAKSESNNQAVSDNKSQQTNKVTKHNETPKQASKAKELPKTGLTSVDNFISTVAFATLALLGSLSLLLFKRKESK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Simon R Clarke, Michael D Wiltshire, Simon J Foster
IsdA of Staphylococcus aureus is a broad spectrum, iron-regulated adhesin.
Mol. Microbiol.: 2004, 51(5);1509-19
[PubMed:14982642] [WorldCat.org] [DOI] (P p)
Christie L Vermeiren, Mark Pluym, John Mack, David E Heinrichs, Martin J Stillman
Characterization of the heme binding properties of Staphylococcus aureus IsdA.
Biochemistry: 2006, 45(42);12867-75
[PubMed:17042505] [WorldCat.org] [DOI] (P p)
Mark Pluym, Naomi Muryoi, David E Heinrichs, Martin J Stillman
Heme binding in the NEAT domains of IsdA and IsdC of Staphylococcus aureus.
J. Inorg. Biochem.: 2008, 102(3);480-8
[PubMed:18194816] [WorldCat.org] [DOI] (P p)