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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1192 [new locus tag: SACOL_RS06105 ]
  • pan locus tag?: SAUPAN003449000
  • symbol: mraW
  • pan gene symbol?: mraW
  • synonym:
  • product: S-adenosyl-methyltransferase MraW

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1192 [new locus tag: SACOL_RS06105 ]
  • symbol: mraW
  • product: S-adenosyl-methyltransferase MraW
  • replicon: chromosome
  • strand: +
  • coordinates: 1196309..1197244
  • length: 936
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGTTTCATCATATCAGCGTTATGTTAAACGAAACCATTGATTATTTAAATGTAAAAGAA
    AATGGTGTGTACATTGACTGTACGCTAGGTGGAGCGGGACATGCCCTTTATTTACTAAAT
    CAATTAAATGACGACGGAAGATTAATAGCAATCGATCAAGACCAAACTGCAATTGATAAT
    GCTAAAGAGGTATTAAAGGATCATTTGCATAGGGTGACTTTTGTTCATAGCAACTTCCGT
    GAATTAACTCAAATATTAAAAGACTTAAACATTGAAAAAGTAGATGGAATTTATTACGAC
    TTGGGTGTTTCAAGCCCACAACTCGACATTCCAGAACGAGGATTCAGTTATCACCATGAC
    GCAACATTAGACATGCGTATGGACCAAACACAAGAACTAACAGCATATGAAATTGTTAAC
    AATTGGTCATATGAAGCGTTAGTGAAGATTTTTTATCGCTATGGCGAGGAGAAATTTTCA
    AAACAGATAGCTCGAAGAATCGAAGCACATCGCGAACAACAACCAATAACAACAACATTA
    GAATTAGTTGACATTATAAAAGAAGGTATTCCTGCAAAAGCAAGAAGAAAAGGCGGACAT
    CCTGCAAAACGAGTATTTCAAGCACTACGAATTGCAGTAAACGATGAATTGTCAGCTTTT
    GAAGATTCAATAGAACAAGCGATTGAATTAGTGAAAGTAGATGGCAGGATTTCGGTAATC
    ACTTTCCATTCTTTAGAAGATCGTTTATGTAAACAGGTGTTCCAAGAATATGAAAAAGGT
    CCAGAGGTACCAAGAGGATTACCAGTTATACCAGAAGCATATACACCTAAGTTAAAGCGT
    GTTAATCGTAAACCGATTACCGCTACAGAAGAAGATTTAGATGACAATAACAGAGCACGA
    AGCGCGAAATTACGTGTAGCTGAAATACTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1192 [new locus tag: SACOL_RS06105 ]
  • symbol: MraW
  • description: S-adenosyl-methyltransferase MraW
  • length: 311
  • theoretical pI: 5.97721
  • theoretical MW: 35709.3
  • GRAVY: -0.454984

Function[edit source | edit]

  • reaction:
    EC 2.1.1.199?  ExPASy
    16S rRNA (cytosine1402-N4)-methyltransferaseS-adenosyl-L-methionine + cytosine1402 in 16S rRNA = S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modification16S rRNA (cytosine(1402)-N(4))-methyltransferase (TIGR00006; EC 2.1.1.199; HMM-score: 409.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonedemethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 14.6)
  • TheSEED:  
    Bacterial Cell Division rRNA small subunit methyltransferase H 
  • PFAM:
    NADP_Rossmann (CL0063) Methyltransf_5; MraW methylase family (PF01795; HMM-score: 456.7)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 31.9)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 22)
    rRNA_methylase; Putative rRNA methylase (PF06962; HMM-score: 21.3)
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 18.5)
    Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 17.6)
    Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 16.7)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 14.6)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 14.5)
    GCD14; tRNA methyltransferase complex GCD14 subunit (PF08704; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 25
    • Cmax: 0.12
    • Cmax_pos: 25
    • Smax: 0.157
    • Smax_pos: 15
    • Smean: 0.091
    • D: 0.102
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLNDDGRLIAIDQDQTAIDNAKEVLKDHLHRVTFVHSNFRELTQILKDLNIEKVDGIYYDLGVSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFSKQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELSAFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTPKLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 179 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 7.6 h [5]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]