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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1410 [new locus tag: SACOL_RS07175 ]
  • pan locus tag?: SAUPAN003769000
  • symbol: femA
  • pan gene symbol?: femA
  • synonym:
  • product: femA protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1410 [new locus tag: SACOL_RS07175 ]
  • symbol: femA
  • product: femA protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1420810..1422111
  • length: 1302
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAATTAATTAACGAGAGACAAATAGGAGTAATGATAATGAAGTTTACAAATTTAACA
    GCTAAAGAGTTTGGTGCCTTTACAGATAGCATGCCATACAGTCATTTCACGCAAACTGTT
    GGCCACTATGAGTTAAAGCTTGCTGAAGGTTATGAAACACATTTAGTGGGAATAAAAAAC
    AATAATAACGAGGTCATTGCAGCTTGCTTACTTACTGCTGTACCTGTTATGAAAGTGTTC
    AAGTATTTTTATTCAAATCGCGGTCCAGTGATTGATTATGAAAATCAAGAACTCGTACAC
    TTTTTCTTTAATGAATTATCAAAATATGTTAAAAAACATCGTTGTCTATACCTACATATC
    GATCCATATTTACCATATCAATACTTGAATCATGATGGCGAGATTACAGGTAATGCTGGT
    AATGATTGGTTCTTTGATAAAATGAGTAACTTAGGATTTGAACATACTGGATTCCATAAA
    GGATTTGATCCTGTGCTACAAATTCGTTATCACTCAGTGTTAGATTTAAAAGATAAAACA
    GCAGATGACATCATTAAAAATATGGATGGACTTAGAAAAAGAAACACGAAAAAAGTTAAA
    AAGAATGGTGTTAAAGTAAGATTTTTATCTGAAGAAGAACTACCAATTTTTAGATCATTT
    ATGGAAGATACGTCAGAATCAAAAGCTTTTGCTGATCGTGATGACAAATTTTACTACAAT
    CGCTTAAAATATTACAAAGACCGTGTGTTAGTACCTTTAGCGTATATCAACTTTGATGAA
    TATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAATAAAGATTTAAATAAAGCGTTA
    AAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACATAACAAGCGAGATAACTTACAA
    CAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGTAAACGTCTACAAGAAGAACAT
    GGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATCAATCCATTTGAAGTTGTTTAT
    TATGCTGGTGGTACATCAAATGCATTCCGTCATTTTGCCGGAAGTTATGCAGTGCAATGG
    GAAATGATTAATTATGCATTAAATCATGGCATTGACCGTTATAATTTCTATGGTGTTAGT
    GGTAAATTTACAGAAGATGCTGAAGATGCTGGTGTAGTTAAATTCAAAAAAGGTTACAAT
    GCTGAAATTATTGAATATGTTGGTGACTTTATTAAACCAATTAATAAACCTGTTTACGCA
    GCATATACCGCACTTAAAAAAGTTAAAGACAGAATTTTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1302

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1410 [new locus tag: SACOL_RS07175 ]
  • symbol: FemA
  • description: femA protein
  • length: 433
  • theoretical pI: 7.8089
  • theoretical MW: 50650.2
  • GRAVY: -0.582448

Function[edit source | edit]

  • reaction:
    EC 2.3.2.17?  ExPASy
    N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferaseMurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNA = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNA
  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage major capsid protein, HK97 family (TIGR01554; HMM-score: 4)
  • TheSEED:  
    Cell Wall and CapsuleCell Wall and Capsule - no subcategorytRNA-dependent amino acid transfers tRNA-dependent lipid II-Gly--glycine ligase 
    Cell Wall and Capsule - no subcategorytRNA-dependent amino acid transfers tRNA-dependent lipid II-GlyGly--glycine ligase 
    tRNA-dependent amino acid transfers FemA, factor essential for methicillin resistance 
  • PFAM:
    Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 508.8)
    Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 16.2)
    no clan definedFlxA; FlxA-like protein (PF14282; HMM-score: 11.4)
    FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 7.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.175
    • Ymax_pos: 22
    • Cmax: 0.12
    • Cmax_pos: 27
    • Smax: 0.385
    • Smax_pos: 11
    • Smean: 0.277
    • D: 0.215
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKLINERQIGVMIMKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVGIKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRCLYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSVLDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFADRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPENKKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSNAFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIEYVGDFIKPINKPVYAAYTALKKVKDRIF

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 376 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 21.26 h [5]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

S Johnson, D Krüger, H Labischinski
FemA of Staphylococcus aureus: isolation and immunodetection.
FEMS Microbiol. Lett.: 1995, 132(3);221-8
[PubMed:7590176] [WorldCat.org] (P p)