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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07175 [old locus tag: SACOL1410 ]
  • pan locus tag?: SAUPAN003769000
  • symbol: SACOL_RS07175
  • pan gene symbol?: femA
  • synonym:
  • product: aminoacyltransferase FemA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07175 [old locus tag: SACOL1410 ]
  • symbol: SACOL_RS07175
  • product: aminoacyltransferase FemA
  • replicon: chromosome
  • strand: +
  • coordinates: 1420849..1422111
  • length: 1263
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1420849..1422111) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAGTTTACAAATTTAACAGCTAAAGAGTTTGGTGCCTTTACAGATAGCATGCCATAC
    AGTCATTTCACGCAAACTGTTGGCCACTATGAGTTAAAGCTTGCTGAAGGTTATGAAACA
    CATTTAGTGGGAATAAAAAACAATAATAACGAGGTCATTGCAGCTTGCTTACTTACTGCT
    GTACCTGTTATGAAAGTGTTCAAGTATTTTTATTCAAATCGCGGTCCAGTGATTGATTAT
    GAAAATCAAGAACTCGTACACTTTTTCTTTAATGAATTATCAAAATATGTTAAAAAACAT
    CGTTGTCTATACCTACATATCGATCCATATTTACCATATCAATACTTGAATCATGATGGC
    GAGATTACAGGTAATGCTGGTAATGATTGGTTCTTTGATAAAATGAGTAACTTAGGATTT
    GAACATACTGGATTCCATAAAGGATTTGATCCTGTGCTACAAATTCGTTATCACTCAGTG
    TTAGATTTAAAAGATAAAACAGCAGATGACATCATTAAAAATATGGATGGACTTAGAAAA
    AGAAACACGAAAAAAGTTAAAAAGAATGGTGTTAAAGTAAGATTTTTATCTGAAGAAGAA
    CTACCAATTTTTAGATCATTTATGGAAGATACGTCAGAATCAAAAGCTTTTGCTGATCGT
    GATGACAAATTTTACTACAATCGCTTAAAATATTACAAAGACCGTGTGTTAGTACCTTTA
    GCGTATATCAACTTTGATGAATATATTAAAGAACTAAACGAAGAGCGTGATATTTTAAAT
    AAAGATTTAAATAAAGCGTTAAAGGATATTGAAAAACGTCCTGAAAATAAAAAAGCACAT
    AACAAGCGAGATAACTTACAACAACAACTTGATGCAAATGAGCAAAAGATTGAAGAAGGT
    AAACGTCTACAAGAAGAACATGGTAATGAATTACCTATCTCTGCTGGTTTCTTCTTTATC
    AATCCATTTGAAGTTGTTTATTATGCTGGTGGTACATCAAATGCATTCCGTCATTTTGCC
    GGAAGTTATGCAGTGCAATGGGAAATGATTAATTATGCATTAAATCATGGCATTGACCGT
    TATAATTTCTATGGTGTTAGTGGTAAATTTACAGAAGATGCTGAAGATGCTGGTGTAGTT
    AAATTCAAAAAAGGTTACAATGCTGAAATTATTGAATATGTTGGTGACTTTATTAAACCA
    ATTAATAAACCTGTTTACGCAGCATATACCGCACTTAAAAAAGTTAAAGACAGAATTTTT
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07175 [old locus tag: SACOL1410 ]
  • symbol: SACOL_RS07175
  • description: aminoacyltransferase FemA
  • length: 420
  • theoretical pI: 7.48443
  • theoretical MW: 49123.3
  • GRAVY: -0.614762

Function[edit source | edit]

  • reaction:
    EC 2.3.2.17?  ExPASy
    N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferaseMurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNA = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNA
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationserine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 7.5)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage major capsid protein, HK97 family (TIGR01554; HMM-score: 4.1)
  • TheSEED:
  • PFAM:
    Acetyltrans (CL0257) FemAB; FemAB family (PF02388; HMM-score: 509)
    Acetyltransf_6; Acetyltransferase (GNAT) domain (PF13480; HMM-score: 16.2)
    no clan definedFlxA; FlxA-like protein (PF14282; HMM-score: 11.5)
    FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 7.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 38
    • Cmax: 0.119
    • Cmax_pos: 55
    • Smax: 0.189
    • Smax_pos: 1
    • Smean: 0.127
    • D: 0.125
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446595963 NCBI
  • RefSeq: WP_000673309 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVGIKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRCLYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSVLDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRFLSEEELPIFRSFMEDTSESKAFADRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPENKKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSNAFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIEYVGDFIKPINKPVYAAYTALKKVKDRIF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07175 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]