From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1628 [new locus tag: SACOL_RS08300 ]
  • pan locus tag?: SAUPAN004153000
  • symbol: SACOL1628
  • pan gene symbol?: phoH
  • synonym:
  • product: PhoH family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1628 [new locus tag: SACOL_RS08300 ]
  • symbol: SACOL1628
  • product: PhoH family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1657881..1658801
  • length: 921
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAACCAATCTCAAGCTTTAATTGGAAATAATGATGAACATTTAAAAGCAATTGAAGAG
    AGTTTCGATGTTGTCATCCATGCAAGAGGACAAGAAGTTGCCGTTAAAGGTACAAAAATA
    GAAAACGTAGAAAAAGCGGAATCAGTATTAATCAATTTGCTGAAGGTTATTGATTTAGGT
    AATAATATTACAATTAAAGATGTTGAAGCAGCTATTAAAATGGCGCATAATAACACAATT
    CAACATCTGTTAGATTTATATGATGAAGAGATAACTAAAGATGCATTTGGTAAGACGATT
    CGTGCGAAAACGATGGGGCAACGTATATATGTTAATGCCATGAAAAATAATGATTTAGTA
    TTTGGTATAGGTCCTGCTGGTACAGGTAAGACATTCTTAGCTGTAGTTTATGCAGCAAAG
    CAACTCCGTAAAGGTGCTGTTAAACGTATTGTATTAACAAGACCTGCTGTTGAAGCAGGA
    GAGTCACTTGGATTTTTACCAGGAGATTTGAAAGAAAAGGTAGATCCATATTTAAGACCT
    TTATATGATGGTCTATATACTGTTCTTGGGCGTGAACAAACAGAGCGATTTATTGAAAGA
    GGCATTATCGAAATAGCGCCACTTGCATATATGCGCGGACGAACATTAGAAGATGCATTT
    GTAATTCTTGATGAGGCGCAGAATACGACACATGCGCAAATGAAAATGTTTTTAACAAGA
    CTAGGTTTTGGCTCAAAAATGGTAGTTACTGGTGACCAAACTCAAATCGATTTACCTAAA
    GGTGTTAAAAGTGGACTTAAGGAAGCGGTCAGTAGGTTACACAACGTTAAAGGTATAAGT
    ATATTGAAATTAGATCAGAGCGATGTAGTCAGACATCCATTGGTAAGTAAGATCATTGAA
    CATTATGAAGGAGAGAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1628 [new locus tag: SACOL_RS08300 ]
  • symbol: SACOL1628
  • description: PhoH family protein
  • length: 306
  • theoretical pI: 7.95346
  • theoretical MW: 33930
  • GRAVY: -0.180065

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairputative DNA helicase (TIGR00376; EC 3.6.4.12; HMM-score: 16.9)
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryPhosphate metabolism Phosphate starvation-inducible protein PhoH, predicted ATPase 
    CBSS-56780.10.peg.1536 Phosphate starvation-inducible protein PhoH, predicted ATPase 
  • PFAM:
    P-loop_NTPase (CL0023) PhoH; PhoH-like protein (PF02562; HMM-score: 314.8)
    AAA_30; AAA domain (PF13604; HMM-score: 35.1)
    AAA_19; AAA domain (PF13245; HMM-score: 24.9)
    AAA_22; AAA domain (PF13401; HMM-score: 19)
    KH (CL0007) KH_1; KH domain (PF00013; HMM-score: 17.8)
    P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 16.8)
    UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 13.8)
    Zot; Zonular occludens toxin (Zot) (PF05707; HMM-score: 13.6)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 13.6)
    no clan definedVps16_C; Vps16, C-terminal region (PF04840; HMM-score: 12.8)
    P-loop_NTPase (CL0023) AAA_25; AAA domain (PF13481; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.106
    • Ymax_pos: 46
    • Cmax: 0.133
    • Cmax_pos: 18
    • Smax: 0.123
    • Smax_pos: 34
    • Smean: 0.082
    • D: 0.097
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAVKGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITKDAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIVLTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLAYMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLKEAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 176 [4]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]