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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1716 [new locus tag: SACOL_RS08765 ]
  • pan locus tag?: SAUPAN004284000
  • symbol: hemD
  • pan gene symbol?: hemD
  • synonym:
  • product: uroporphyrinogen III synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1716 [new locus tag: SACOL_RS08765 ]
  • symbol: hemD
  • product: uroporphyrinogen III synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 1747032..1747700
  • length: 669
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAGCCAGTTGTAGTTATGACACAAACAAATGACATGCAAAGTGATTTAGTATCAATT
    ATTCACAAACCCTTTATTGATATAAAGCCACTAAATTTTGATATTCATTTGTTAAATCAA
    CGCTATGACTGGCTTATTTTTTCATCTAAAAATGCTGTGAAATTCTTTTATAAATATCTA
    AAAGGAATTAATGTTGATAACATTGCTGTGATAGGAAGTAAGACAGCGCAATATTGTGAA
    TCACTTGGCATTCGAGTTGATTTTATGCCAAACGACTTTTCTCAAGAAGGATTTTTAAAA
    TCATTTAATCAAACTAACCAAAAAATACTTTTGCCTTCGAGTGAATTGGCGAGACCATTG
    TTATTAGCAGCGTTATCTAAAGATAATGAAGTTGTTAAAATAGATTTATATACTTCAGTG
    CCTAACAAACAAAATATACAAGATGTTAAAGAAATGATAGAACATCAACAAATCGATGCA
    TTAACATTTTCAAGTTCGTCGGCAGTACGTTATTATTTTAATGAAGGATTTGTACCAAAA
    TTCAAGTCGTATTTTGCTATTGGAGAACAAACAGCACGGACCATTAAATCATATCAACAA
    CCAGTAACAATTGCAGAAATTCAAACACTCGAATCACTAATTGAAAAGATTTTAGAAAGT
    AGGGGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    669

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1716 [new locus tag: SACOL_RS08765 ]
  • symbol: HemD
  • description: uroporphyrinogen III synthase
  • length: 222
  • theoretical pI: 7.58445
  • theoretical MW: 25418.1
  • GRAVY: -0.167117

Function[edit source | edit]

  • reaction:
    EC 4.2.1.75?  ExPASy
    Uroporphyrinogen-III synthaseHydroxymethylbilane = uroporphyrinogen III + H2O
  • TIGRFAM:
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsTetrapyrrolesCPO analysis Uroporphyrinogen-III synthase (EC 4.2.1.75) 
  • PFAM:
    no clan definedHEM4; Uroporphyrinogen-III synthase HemD (PF02602; HMM-score: 123.7)
    HHH (CL0198) IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.122
    • Ymax_pos: 21
    • Cmax: 0.114
    • Cmax_pos: 21
    • Smax: 0.159
    • Smax_pos: 14
    • Smean: 0.127
    • D: 0.124
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDWLIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSFNQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDALTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKILESRG

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]