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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1880 [new locus tag: SACOL_RS09650 ]
  • pan locus tag?: SAUPAN004583000
  • symbol: lukD
  • pan gene symbol?: lukD
  • synonym:
  • product: leukotoxin LukD

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1880 [new locus tag: SACOL_RS09650 ]
  • symbol: lukD
  • product: leukotoxin LukD
  • replicon: chromosome
  • strand: -
  • coordinates: 1934671..1935654
  • length: 984
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAAATGAAAAAATTAGTCAAATCATCAGTTGCTTCATCAATTGCACTGCTTTTGCTA
    TCGAATACAGTTGATGCAGCTCAACATATCACACCTGTAAGCGAGAAAAAAGTAGATGAC
    AAAATCACTTTATACAAAACAACAGCAACATCTGATAATGATAAATTGAATATTTCTCAA
    ATTTTAACGTTTAATTTCATTAAGGATAAAAGTTATGACAAAGATACGTTAGTACTTAAG
    GCAGCCGGAAACATTAATTCAGGTTATAAAAAGCCTAATCCAAAAGATTACAATTACTCA
    CAGTTTTATTGGGGCGGTAAGTATAATGTTTCGGTTAGTTCAGAATCAAATGATGCTGTA
    AATGTTGTTGACTATGCACCTAAAAATCAAAATGAAGAATTCCAAGTTCAACAAACATTA
    GGTTATTCTTATGGCGGAGATATTAATATATCTAATGGCTTATCAGGTGGATTAAATGGA
    TCAAAATCATTTTCAGAAACGATAAATTATAAACAAGAAAGTTACAGAACTACGATTGAT
    AGAAAAACAAATCATAAATCAATTGGCTGGGGTGTTGAGGCGCACAAAATTATGAATAAT
    GGTTGGGGACCATATGGTAGAGATAGTTATGACCCAACATATGGTAATGAACTGTTTTTA
    GGCGGTAGACAAAGTAGTTCAAATGCTGGTCAAAATTTCTTGCCAACACATCAAATGCCT
    TTATTGGCGCGTGGTAACTTTAACCCAGAATTTATAAGCGTACTTTCTCATAAACAAAAT
    GATACAAAAAAATCTAAAATCAAAGTAACTTACCAAAGAGAAATGGATAGATATACTAAT
    CAATGGAATCGACTACACTGGGTTGGTAATAACTACAAAAATCAAAATACAGTAACGTTT
    ACATCTACTTATGAAGTTGACTGGCAAAACCATACTGTTAAATTAATCGGTACGGATTCT
    AAAGAAACTAATCCTGGAGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

Protein[edit source | edit]

Protein Data Bank: 4Q7G

General[edit source | edit]

  • locus tag: SACOL1880 [new locus tag: SACOL_RS09650 ]
  • symbol: LukD
  • description: leukotoxin LukD
  • length: 327
  • theoretical pI: 9.65379
  • theoretical MW: 36888.7
  • GRAVY: -0.815596

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancebeta-channel forming cytolysin (TIGR01002; HMM-score: 441.5)
  • TheSEED:  
    Virulence, Disease and DefenseToxins and superantigensStaphylococcus Two-component and Pore-forming Cytolysins Leukotoxin LukD 
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 296.9)
    no clan definedtRNA_synt_1c_R2; Glutaminyl-tRNA synthetase, non-specific RNA binding region part 2 (PF04557; HMM-score: 13.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NTVDAAQH
  • SignalP: Signal peptide length 27 aa
    • Ymax: 0.645
    • Ymax_pos: 27
    • Cmax: 0.446
    • Cmax_pos: 27
    • Smax: 0.969
    • Smax_pos: 8
    • Smean: 0.943
    • D: 0.761
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITLYKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQFYWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNGSKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNELFLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMDRYTNQWNRLHWVGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SaeR
    SaeR (TF) important in VirulenceRegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]