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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
  • pan locus tag?: SAUPAN004850000
  • symbol: mutY
  • pan gene symbol?: mutY
  • synonym:
  • product: A/G-specific adenine glycosylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
  • symbol: mutY
  • product: A/G-specific adenine glycosylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1990591..1991628
  • length: 1038
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTATCAGCAGTCTAGTTTTAAAGAAAATTTGATACACTGGTTTGATGAAAATCAAAGA
    GAAATGCCATGGCGACAAACGACCAATCCGTACTATATTTGGTTAAGTGAGGTCATGTTA
    CAACAGACACAAGTTAAAACAGTTATTGACTATTATCATCGTTTTGTTGAACGATTTCCA
    ACTGTGGAAGTTTTGAGTCAAGCTTCGGAAGATGAAGTGTTAAAGTATTGGGAAGGCTTA
    GGATATTATAGTAGAGCTCGAAATTTTCATACGGCGATTAAAGAAGTTCATGATAAATAT
    GAAGGCTTAGTTCCCAAAGATCCTGATCAATTTAAAGCGTTAAAAGGTGTTGGCCCATAC
    ACACAAGCTGCAGTAATGAGCATTGCATATAATGTACCACTAGCAACTGTAGACGGAAAT
    GTTTTTCGAGTATGGTCTAGATTGAATGATGACTATCGAGATATTAAATTACAATCGACG
    AGGAAATCGTACGAACAAGAATTATTACCGTATGTTACTACAGAAGCTGGAACGTTTAAT
    CAAGCTATGATGGAATTAGGGGCGTTAATTTGTACACCAAAAAATCCATTATGCTTATTT
    TGTCCAGTTCAAGAAAACTGTGAGGCATTTGATAAAGGTACATTTGAAAAGTTACCTGTT
    AAATCTAAAAATGTAAGTAAAAAAGTAATTGAGCAATCCGTCTTTTTAATTAGAAATAAT
    CAAGGTCAATATCTGTTGCAAAAGCGTAGCGAAAAATTATTGCATGGTATGTGGCAATTT
    CCTATGTTTGAAAGTGAGCATGCTAGACGTAAAATGACTGAAAAAATCGGTCATGACATT
    CAACCAGTAGAAACACCTATTTTTGAGTTGAAGCATCAATTTACTCATTTAACATGGAAG
    ATTAAAGTATACGCCGTTTCAGGAGCAATTAACATTGAAACATTACCAGATGACATGATT
    TGGTTTGATTTAAGTGATCGAGATCAATATACATTCCCTGTACCTATGTCAAAGATTTAT
    CAATTTATAAATGGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1038

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1926 [new locus tag: SACOL_RS10070 ]
  • symbol: MutY
  • description: A/G-specific adenine glycosylase
  • length: 345
  • theoretical pI: 6.79462
  • theoretical MW: 40623.2
  • GRAVY: -0.495652

Function[edit source | edit]

  • reaction:
    EC 3.2.2.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairA/G-specific adenine glycosylase (TIGR01084; EC 3.2.2.-; HMM-score: 328.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairendonuclease III (TIGR01083; EC 4.2.99.18; HMM-score: 74.1)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, bacterial A/G-specific adenine glycosylase (EC 3.2.2.-) 
    CBSS-176280.1.peg.1561 A/G-specific adenine glycosylase (EC 3.2.2.-) 
    CBSS-261594.1.peg.788 A/G-specific adenine glycosylase (EC 3.2.2.-) 
  • PFAM:
    NUDIX (CL0261) NUDIX_4; NUDIX domain (PF14815; HMM-score: 78.6)
    HHH (CL0198) HhH-GPD; HhH-GPD superfamily base excision DNA repair protein (PF00730; HMM-score: 55.7)
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 28.1)
    no clan definedEndIII_4Fe-2S; Iron-sulfur binding domain of endonuclease III (PF10576; HMM-score: 16.9)
    NUDIX (CL0261) NUDIX; NUDIX domain (PF00293; HMM-score: 15.4)
    no clan definedNDUFB10; NADH-ubiquinone oxidoreductase subunit 10 (PF10249; HMM-score: 12.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.1
    • Ymax_pos: 69
    • Cmax: 0.119
    • Cmax_pos: 69
    • Smax: 0.113
    • Smax_pos: 68
    • Smean: 0.066
    • D: 0.087
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQVKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEGLVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQSTRKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKLPVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKIGHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPVPMSKIYQFING

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1]
    quantitative data / protein copy number per cell: 35 [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]