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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1975 [new locus tag: SACOL_RS10325 ]
  • pan locus tag?: SAUPAN004946000
  • symbol: SACOL1975
  • pan gene symbol?:
  • synonym:
  • product: nicotinate phosphoribosyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1975 [new locus tag: SACOL_RS10325 ]
  • symbol: SACOL1975
  • product: nicotinate phosphoribosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2037975..2039444
  • length: 1470
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    GTGTACCAATTAGAAGACGACAGTTTAATGTTACATAATGACTTATATCAAATAAATATG
    GCTGAAAGTTATTGGAATGATAATATTCATGAAAAAATGGCTGTATTTGATTTGTATTTT
    AGAAAAATGCCATTTAATAGTGGCTATGCTGTTTTTAATGGTTTAAAACGCGTCATTGAT
    TTTATAGAACATTTTGGATTTTCAGAATCTGATTTGGAATATTTAAAGTCTATTGGCTAC
    AAGGATGATTTCTTATCATATTTAAAAGATTTAAAATTCACAGGCAGCATCCGTTCGATG
    CAAGAAGGCGAATTATGCTTTGGTAACGAACCATTGTTACGCGTAGAAGCACCATTGATT
    CAAGCGCAATTAATAGAAACAATTTTATTAAACATTGTAAATTTCCATACATTAATTACA
    ACAAAGGCTAGCAGAATTCGTCAAATTGCATCAAATGATAAATTAATGGAGTTTGGTACA
    CGTCGTGCGCAAGAAATTGATGCAGCATTGTGGGGCGCTAGAGCTGCTTACATCGGGGGC
    TTTGATTCTACAAGTAATGTTAGGGCGGGGAAATTATTTGGTATACCTGTGTCTGGTACA
    CATGCACATGCATTTGTCCAAACTTATGGAGACGAATATGTTGCCTTCAAAAAATATGCT
    GAAAGACATAAAAATTGTGTGTTCCTAGTAGATACATTCCATACTTTAAAATCTGGCGTG
    CCAAATGCAATAAAAGTTGCAAAAGAATTAGGTGACAAAATTAACTTTGTAGGTATTCGA
    TTAGATTCTGGAGATATCGCTTATTTATCTAAAGAGGCAAGACGTATGCTTGATGAAGCA
    GGATTTACTGAAACTAAAATTATCGCGTCTAATGATTTGGATGAAGAAACGATTACGAGT
    TTGAAAGCACAAGGTGCAAAAGTAGATTCTTGGGGCGTTGGTACAAAGCTGATTACAGGA
    TACGATCAACCAGCATTAGGTGCAGTATATAAACTTGTAGCTATTGAAAATGAAGATGGT
    TCATATAGTGATCGTATTAAATTATCAAATAACGCTGAAAAGGTTACGACGCCAGGTAAG
    AAAAATGTATATCGCATTATAAACAAGAAAACAGGTAAGGCAGAAGGCGATTATATTACT
    TTGGAAAATGAAAATCCATACGATGAACAACCTTTAAAATTATTCCATCCAGTGCATACT
    TATAAAATGAAATTTATAAAATCTTTCGAAGCCATTGATTTGCATCATAATATTTATGAA
    AATGGTAAATTAGTATATCAAATGCCAACAGAAGATGAATCACGTGAATATTTAGCACTA
    GGATTACAATCTATTTGGGATGAAAATAAGCGTTTCCTGAATCCACAAGAATATCCAGTC
    GATTTAAGCAAGGCATGTTGGGATAATAAACATAAACGTATTTTTGAAGTTGCGGAACAC
    GTTAAGGAGATGGAAGAAGATAATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1470

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1975 [new locus tag: SACOL_RS10325 ]
  • symbol: SACOL1975
  • description: nicotinate phosphoribosyltransferase
  • length: 489
  • theoretical pI: 5.51423
  • theoretical MW: 55891.9
  • GRAVY: -0.434151

Function[edit source | edit]

  • reaction:
    EC 6.3.4.21?  ExPASy
    Nicotinate phosphoribosyltransferaseNicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
    EC 2.4.2.11?  ExPASy
    Transferred entry: 6.3.4.21
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesnicotinate phosphoribosyltransferase (TIGR01513; EC 6.3.4.21; HMM-score: 596.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesnicotinate phosphoribosyltransferase (TIGR01514; EC 6.3.4.21; HMM-score: 63.6)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsNAD and NADPNAD and NADP cofactor biosynthesis global Nicotinate phosphoribosyltransferase (EC 2.4.2.11) 
    Stress ResponseOxidative stressRedox-dependent regulation of nucleus processes Nicotinate phosphoribosyltransferase (EC 2.4.2.11) 
  • PFAM:
    TIM_barrel (CL0036) NAPRTase; Nicotinate phosphoribosyltransferase (NAPRTase) family (PF04095; HMM-score: 41.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL1513(hup)DNA-binding protein HU  [1] (data from MRSA252)
    SACOL0584(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SACOL2236(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SACOL2239(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SACOL2227(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    SACOL2224(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    SACOL2220(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SACOL2232(rplP)50S ribosomal protein L16  [1] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SACOL1702(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SACOL2234(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SACOL1769(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    SACOL2214(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SACOL2230(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.095
    • Ymax_pos: 24
    • Cmax: 0.124
    • Cmax_pos: 24
    • Smax: 0.111
    • Smax_pos: 20
    • Smean: 0.061
    • D: 0.082
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMPFNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQEGELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGTRRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKKYAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRMLDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVAIENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPLKLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENKRFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [2] [3] [4]
    quantitative data / protein copy number per cell: 474 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 20.63 h [6]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]