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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2181 [new locus tag: SACOL_RS11475 ]
  • pan locus tag?: SAUPAN005586000
  • symbol: lacE
  • pan gene symbol?: lacE
  • synonym:
  • product: PTS system, lactose-specific IIBC components

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2181 [new locus tag: SACOL_RS11475 ]
  • symbol: lacE
  • product: PTS system, lactose-specific IIBC components
  • replicon: chromosome
  • strand: -
  • coordinates: 2263934..2265652
  • length: 1719
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGACAATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAA
    TTATCACGTAACATATATTTAAGAGCCATCCGTGATGGATTTATTTCTGCAATGCCTGTC
    ATCTTATTCTCAAGTATCTTTCTATTAATTGCATATGTGCCAAATATATTTGGTTTTAAA
    TGGGATAAAGGCATGGAAGCCATTTTAATGAAACCATATAACTATACGATGGGGCTTGTA
    GCATTTTTAGTAGCAGGAACGACAGCGAAATCTTTAACAGATTCATTTAACCGTAAGCTA
    GAAAGTACAAATCAAATTAACTTTATTTCTACAATGCTAGCAGCGATGTGTGGATTCTTG
    TTCTTAGCTTCTGATCCAGCCAAAGATGGTGGATTCTTAAGTGCATTTATGGGAACTAAA
    GGTTTATTAACAGCATTCTTATCAGCATTTGTAACTGTTATTGTTTATAACTTCTGTGTG
    AAGCGCAATATTACAATTAAAATGCCGAAAGAAGTACCGCCGAATATTTCACAAGTATTT
    AAGGACTTAATTCCATTTTCAGCGGTAATCATCATTCTTTATGCATTAGATTTAGTCATT
    CGCAACAGCTTTAAATCAAATGTAGCGGAAGGTATTTTAAAATTATTCGAACCATTATTT
    ACAGCAGCAGATGGATGGATTGGTGTCACAATTATCTTTGGTGCCTTTGCATTATTCTGG
    TTTGTAGGTATTCATGGTCCGTCAATTGTAGAGCCAGCAATTGCAGCCATTACATATGCG
    AATATCGAAGCGAACTTCAAGTTGCTTCAAGCTGGAGAACACGCAGATAAAATTATTACA
    TCAGGTACACAAATGTTTATCGTTACATTTGGAGGTACGGGTGCAACATTGGTCGTCCCG
    TTCATGTTTATGTGGATGACGAAATCTAAACGAAACAAAGCGATTGGACGTGCATCAGTT
    GTACCAACATTCTTTGGTGTAAACGAACCAATTTTATTTGGTGCACCACTAGTATTGAAT
    CCTGTGTTCTTTATTCCATTTGTATTAGCACCAATTGTTAACGTATGGATTTTCAAACTT
    TTCGTTGAAGTGTTAGGAATGAATAGTTTTAGTGTGAATTTACCTTGGACAACACCAGGT
    CCATTGGGCATTATCATGGGTACAGGTTTTGGTTTATGGTCATTCGTACTAGCTATTACT
    TTGATTGTTGTAGATATTATTATTTACTACCCATTCCTAAAAGTTTATGATAGTGAAATT
    CTTGATGAAGAAGAAGGACGTAAAGAAAGTAATTCAGATTTAAAAGAAAAAGTTGCAGCA
    AACTTTGATACGAAAAAAGCTGATTCAATTTTAGCGGCAAGTGGTGTATCAGACGATGCA
    GCTAAAGCTTCAAATATCACAGAACAAACAAATGTCTTAGTACTTTGTGCCGGTGGTGGT
    ACAAGTGGATTATTAGCCAATGCACTTAATAAAGCAGCTGAAGAATATCATGTGCCAGTG
    AAAGCGGCAGCTGGCGGATATGGCGCACATATGGACATTATGAAAGAGTACCAATTAATC
    ATTTTAGCGCCACAAGTTGCGTCTAACTATGAAGATATTAAGCAAGATACAGATAGATTA
    GGTATTAAATTAGCGAAGACTCAAGGTGCTGAATATATCAAATTGACACGAGATGGCCAA
    GCAGCCCTTGATTTCGTTCAACAACAATTTGAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1719

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2181 [new locus tag: SACOL_RS11475 ]
  • symbol: LacE
  • description: PTS system, lactose-specific IIBC components
  • length: 572
  • theoretical pI: 7.48602
  • theoretical MW: 62664.1
  • GRAVY: 0.443007

Function[edit source | edit]

  • reaction:
    EC 2.7.1.207?  ExPASy
    Protein-N(pi)-phosphohistidine--lactose phosphotransferase[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
    Signal transductionSignal transductionPTSPTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
    Signal transductionSignal transductionPTSPTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
    Signal transductionSignal transductionPTSPTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.3)
    Signal transductionSignal transductionPTSPTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.3)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 35.2)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 26.3)
  • TheSEED:  
    Lactose and Galactose Uptake and Utilization PTS system, lactose-specific IIB component (EC 2.7.1.69) / PTS system, lactose-specific IIC component (EC 2.7.1.69) 
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 253)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 40.7)
    PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 11.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.134
    • Ymax_pos: 15
    • Cmax: 0.125
    • Cmax_pos: 60
    • Smax: 0.238
    • Smax_pos: 9
    • Smean: 0.159
    • D: 0.144
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTMMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKRNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQQFEN

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]