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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2183 [new locus tag: SACOL_RS11485 ]
  • pan locus tag?: SAUPAN005588000
  • symbol: lacD
  • pan gene symbol?: lacD
  • synonym:
  • product: tagatose 1,6-diphosphate aldolase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2183 [new locus tag: SACOL_RS11485 ]
  • symbol: lacD
  • product: tagatose 1,6-diphosphate aldolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2265985..2266965
  • length: 981
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTCAAAATCGAATCAAAAAATCGCATCAATTGAACAATTAAGTAACAACGAAGGTATC
    ATTTCTGCATTAGCATTTGACCAACGTGGTGCTTTAAAACGCATGATGGCAAAACATCAA
    ACTGAAGAACCAACTGTAGCTCAAATTGAACAATTAAAAGTATTAGTTGCGGAAGAATTA
    ACACAATATGCATCTTCAATTTTATTAGATCCTGAATATGGTTTACCAGCTTCAGATGCA
    CGTAACAAAGATTGTGGATTATTACTTGCTTATGAAAAAACTGGATATGACGTAAACGCT
    AAAGGTCGTTTACCTGACTGTTTAGTAGAGTGGTCTGCAAAACGTTTAAAAGAACAAGGC
    GCAAATGCTGTTAAATTCTTACTTTACTATGATGTTGATGATGCTGAAGAAATTAACATT
    CAAAAGAAAGCATACATTGAAAGAATCGGTTCAGAATGTGTTGCAGAAGATATTCCATTC
    TTTTTAGAAGTATTAACATACGATGACAACATTCCTGATAACGGTAGTGTTGAATTCGCA
    AAAGTTAAGCCTAGAAAGGTTAACGAAGCAATGAAATTATTCTCAGAACCACGCTTTAAC
    GTTGATGTATTGAAAGTTGAAGTACCAGTAAACATGAAATATGTAGAAGGCTTTGCTGAA
    GGTGAAGTGGTTTATACAAAAGAGGAAGCGGCACAACACTTTAAAGATCAAGATGCAGCA
    ACTCATTTACCATACATTTACTTAAGTGCTGGTGTTTCTGCGGAATTATTCCAAGAAACT
    TTAAAATTTGCTCACGAAGCTGGTGCTAAATTCAACGGGGTACTTTGTGGCCGTGCTACA
    TGGTCTGGTGCTGTTCAAGTATACATTGAACAAGGTGAAGATGCTGCTCGTGAATGGTTA
    CGTACAACTGGTTTCAAAAATATTGATGACTTAAATAAAGTATTAAAAGATACTGCGACA
    TCTTGGAAACAAAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    981

Protein[edit | edit source]

Protein Data Bank: 3MYP

General[edit | edit source]

  • locus tag: SACOL2183 [new locus tag: SACOL_RS11485 ]
  • symbol: LacD
  • description: tagatose 1,6-diphosphate aldolase
  • length: 326
  • theoretical pI: 4.77016
  • theoretical MW: 36595.2
  • GRAVY: -0.389571

Function[edit | edit source]

  • reaction:
    EC 4.1.2.40?  ExPASy
    Tagatose-bisphosphate aldolase D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides tagatose 1,6-diphosphate aldolase (TIGR01232; EC 4.1.2.40; HMM-score: 717.7)
  • TheSEED  :
    • Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
    and 1 more
    Carbohydrates Monosaccharides D-Tagatose and Galactitol Utilization  Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40)
  • PFAM:
    TIM_barrel (CL0036) DeoC; DeoC/LacD family aldolase (PF01791; HMM-score: 263.6)
    and 2 more
    DUF2090; Uncharacterized protein conserved in bacteria (DUF2090) (PF09863; HMM-score: 26.8)
    no clan defined TIP41; TIP41-like family (PF04176; HMM-score: 12.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003114
    • TAT(Tat/SPI): 0.000468
    • LIPO(Sec/SPII): 0.000295
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEPTVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKGRLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPFFLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGFAEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLCGRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • data available for N315

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]

Sang Jae Lee, Hyoun Sook Kim, Do Jin Kim, Hye-Jin Yoon, Kyoung Hoon Kim, Ji Young Yoon, Se Won Suh
Crystal structures of LacD from Staphylococcus aureus and LacD.1 from Streptococcus pyogenes: insights into substrate specificity and virulence gene regulation.
FEBS Lett: 2011, 585(2);307-12
[PubMed:21192932] [WorldCat.org] [DOI] (I p)