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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2674 [new locus tag: SACOL_RS14005 ]
  • pan locus tag?: SAUPAN006387000
  • symbol: SACOL2674
  • pan gene symbol?: asp1
  • synonym:
  • product: accessory secretory protein Asp1

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL2674 [new locus tag: SACOL_RS14005 ]
  • symbol: SACOL2674
  • product: accessory secretory protein Asp1
  • replicon: chromosome
  • strand: -
  • coordinates: 2745200..2746753
  • length: 1554
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAATACTTTATTCCAGCTTGGTACGATGACCAACGATGGTGGCAAGACACGACTGTG
    CCGTATTATCAACTACAAAATAAGACGGAATTTGACGATATGATTAGTTTAATGGGAATG
    CACCTTGAAAATGACTTAGATTATCAACTGATTGTTCTCAATCATGCACCAAATTTAAGA
    ACATTTTTACATCGATATGACTTATATGAGACAAAGTATTCGTCTGTGTTTGATGAAATT
    CAAGGATTCAGTCACCATGCGCCACAAGCGATTAATTATCATCACTTAAAATGGCCGGAT
    GATGTTGAGTTTGTGTACACACCGTATTTATTAAAATGTGTGACGAGTGAACAGACCTAT
    ACAAATATTTATTTTAGTCAAGAAGGGTATTCAATTTGGTTTGAAGAATTTGAAAGAGAT
    CAGTTACAACGGCGTTATATTTTTGATGACAGAGGTTATTTATCAGCGATACGTTATTTT
    GATGATCAGGGAGAGGCTTCTTACCAAGAATATTTAACGATTAATGGAGATTGTGTACTT
    CATGAAGATTTGAAAAATGGCAGAGTCACTGTTTCAAAAAGATATCAACATCACTATCAA
    CAAACAGAATATAACAATATGGCTCAACTAATTGAAGAAAAATTTCAAGCAATGATTGCA
    CAACAAATACATGAAGATGATCATGTGATTGTGGCTTCAGATGCTAGGCACAATCGACAA
    ATAGCCAATCATATTCCAGCGAAATCATTAAGTTATTCATTTTTTAAAAATAGAAATGAA
    ACTGTGTCAGATGAGGAATATCAATCTATCGTAAAGAATGCCCATTTAATTGTTGATAGT
    GTGCAACTAGAACGTGATTTAATTAGTCATCAAGAGAAGTATCAGCGGGAGAATACAATG
    ATTCGAATCACACCATTTGAAACGAGACAATCACCTAATATAAGTAGTCAATTGATGGAA
    ACATTTATAGGTGTATGGATAGATGGTATGAGTGACGCTGATTTGCAACAAATGATGCAA
    CGACTTGTGGATTATATCGCACAGGAAGATTATTACCGTTTAATTTTATTATCGCGCCAT
    CAAAATGACATACCGATGTGGCTTCGTGAATGTATTACGTCGGTAAATGAGGAATACCAA
    GCTAAACAGAATGCGGATGTTAATGTTTCAGCATTAATGACACCTGAAGATCAAGATGAC
    ATCATTGCTGTTAAGACGATACATGCTGAACATGATGTTGTAGAAGCATTGCGGACGTTG
    CGACTTGTGATAGATATGTCAAAAGAACCTGACTTGTATTTACAAATTAGTGCAATTAGC
    GCTGGGATTCCACAAATTAATGGTCAACAAACAGATTACGTCTCTGATTATGACAATGGC
    CGTATTATAAATACAGTTGATGAATTAGATGATGCGTTAAATTATTATTTATTTTATTTG
    AAAAATTGGAATTATGCGTACGCCTATTCTTTAAAATTAATAGATGCATATGCTTCTAAG
    AATATTATTAATCAGCTCGATGAGTTAATAGAAGGTGAAAATGATGCCACGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL2674 [new locus tag: SACOL_RS14005 ]
  • symbol: SACOL2674
  • description: accessory secretory protein Asp1
  • length: 517
  • theoretical pI: 4.43636
  • theoretical MW: 61258.8
  • GRAVY: -0.585493

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesPathogenesisaccessory Sec system protein Asp1 (TIGR03713; HMM-score: 733.4)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingaccessory Sec system protein Asp1 (TIGR03713; HMM-score: 733.4)
  • TheSEED:  
    Membrane TransportProtein translocation across cytoplasmic membraneSecY2-SecA2 Specialized Transport System Accessory secretory protein Asp1 
  • PFAM:
    GT-B (CL0113) Asp1; Accessory Sec system protein Asp1 (PF16993; HMM-score: 360)
    no clan definedVasohibin; Vasohibin (PF14822; HMM-score: 15.5)
    Ntox16; Novel toxin 16 (PF15523; HMM-score: 13.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.084
    • Ymax_pos: 70
    • Cmax: 0.1
    • Cmax_pos: 33
    • Smax: 0.092
    • Smax_pos: 65
    • Smean: 0.052
    • D: 0.072
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLENDLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDVEFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYFDDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAMIAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHLIVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDADLQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSALMTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQTDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDELIEGENDAT

Experimental data[edit source | edit]

  • experimental localization: Cytoplasmic [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]