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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS01110 [old locus tag: SACOL0217 ]
  • pan locus tag?: SAUPAN001102000
  • symbol: SACOL_RS01110
  • pan gene symbol?: opp-5A
  • synonym:
  • product: nickel ABC transporter substrate-binding protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS01110 [old locus tag: SACOL0217 ]
  • symbol: SACOL_RS01110
  • product: nickel ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 258147..259559
  • length: 1413
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (258147..259559) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCATTCATCTGGCAAAGACTTAAATATTTCGTTACCGTTAAAAACAAAGTCTATTGCA
    CCTTATGAAACAGATGTACCAGTTAAGATTGGTGCTGCAGAATCATTATTTAAAACAAAT
    GATCAAGGAAAAATAGAAAAAGCATTAGTGAAATCGTATCATCAACCAAATGATACAACG
    TTAGATATAGAATTAAAAGATAATATTAAATTTCAAAACGGTCAAAAATTGACTGCAGAA
    AAAGTGAAATCTAGCCTTGAAAATAGCATGAAAAAAAGCGACTTGGTCAAATATTCATTA
    CCAATATCATCAATTACCGCTAAAGGTCAAAAACTGACAATTAAAACCAACTCCGCTTAC
    CCTGAACTTGTATCTGAATTAGCTAATCCTTTTATGGCAATTTATGATACAGATGCTAAA
    TCAGATGTTAATCAAACTCCTGTTGGTACAGGCCCTTACCAAATAAAAGATTATAAGCAA
    TCTCGAAAAATATCATTGTCGAATTTTAAGGACTATTGGCAAGGTAAACCGAAACTTGAT
    CATATTACTGTGACGTACCAAGAAGACGGCAATAATCGCGTCAGAAATTTAGAATCTCAA
    AAAGATGATTTAATAACTGATGTCCCAGTTAATAAAGTTCAAGACATAGAAAATAATCAA
    AATTTAAAAGTGTCAAAAGAATCTGGATTTAGAACTTCTTTACTTATGTATAATCATACT
    AATAAAAAAATGACTAAATCCGTTCGTGAAGCATTAGATCATATCATTGATAGACAAGGT
    ATTGCAGATCATATTTATCAAGGTTATGCGAAACCTGCAACGAGTCCATTTAATGATAAA
    ATTCCATATATTAAAGAACCTAAGTTAACCAAACAAAATATCGAACAAGCAAAAATGTTA
    TTAGCTAAAGATGGTTATACAAAAGAACACCCTTTAAAAATTAAGTTAATTACGTATGAC
    GGTCGTCCAGAGCTATCGAAAATTGCGCAAGTATTACAATCAGATGCTAAAAAAGCAAAT
    ATTGAAATCGACATTAAAAGTGTTGATGACATAGAAGGTTACTTAAAAGACCGTTCTGCA
    TGGGATGCAACGATGTATAGTTTCGGAACAATTCCTCGTGGAGATACAGGTTATTTCTTT
    AATCAAGCATATAAAAAAGATGGTGCTATTAATAAAGGAGACTACAATAATAGCAACGTC
    GATGATTTAATTAATCAATTGAATCATACTGTAGATGTTAAGGAACGTCACAATATTTCT
    AATGATATTATTAAATTATCAAGTCGAGATGTACCAAACAGTTATATTGCTTACAACGAT
    CAAATAGTCGCTGCTAACTCTAAGGTTAAAAATTATAAAGTAACACCTGAAGGTATCTAT
    TTAATCGATTATCGAACAACGATTGAACGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1413

Protein[edit source | edit]

Protein Data Bank: 3RQT

General[edit source | edit]

  • locus tag: SACOL_RS01110 [old locus tag: SACOL0217 ]
  • symbol: SACOL_RS01110
  • description: nickel ABC transporter substrate-binding protein
  • length: 470
  • theoretical pI: 9.38724
  • theoretical MW: 53219.7
  • GRAVY: -0.74617

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsnickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 117.8)
    MetabolismTransport and binding proteinsUnknown substrateABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 42.4)
  • TheSEED:
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 223.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.2
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.161
    • Ymax_pos: 33
    • Cmax: 0.156
    • Cmax_pos: 33
    • Smax: 0.304
    • Smax_pos: 18
    • Smean: 0.185
    • D: 0.171
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_000556246 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS01110 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]