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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS07900 [old locus tag: SACOL1551 ]
  • pan locus tag?: SAUPAN004042000
  • symbol: SACOL_RS07900
  • pan gene symbol?: malA
  • synonym:
  • product: alpha-glucosidase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS07900 [old locus tag: SACOL1551 ]
  • symbol: SACOL_RS07900
  • product: alpha-glucosidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1586830..1588479
  • length: 1650
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1586830..1588479) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
    421
    481
    541
    601
    661
    721
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    841
    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATAAGCAATGGTGGAAAGAAGCAGTAGCATATCAAGTATATCCAAGAAGTTTTAAT
    GATAGTAATCACGATGGTATTGGGGATTTACCTGGAATGATTGATAAATTGGACTACTTA
    AAAGATTTCGGTATCGATGTCATTTGGCTCAGTCCAATGTTTAAATCACCTAATGATGAC
    AATGGTTATGATATTAGTGACTACCAAGAGATTATGGATGAATTTGGAACGATGGAAGAC
    TTTGATCGTTTATTAAAAGGTGTTCATGATAGAGGCATGAAGCTTATTTTAGATTTAGTT
    GTAAATCATACATCTGATGAACATCCTTGGTTTATAGAATCCAAATCTAGTAAAGACAAT
    CCCAAACGTGATTGGTACATTTGGCAAGATCCAAAGCCAGATGGCTCTGAACCTAACAAC
    TGGGAAAGTATATTTAATGGATCTACATGGGAATATGATGCTAATACTGAGCAATATTAT
    TTCCATTTATTCAGTAAAAAACAACCTGATTTGAATTGGGGTAATCCGGAAGTTAGAGAT
    GCTGTATTTGAAATGATGAACTGGTGGTTTGATAAAGGCATTGATGGATTTAGAGTAGAT
    GCAATTACGCATATTAAGAAGACGTTTGAAGCGGGTGACTTACCTGTACCTGAGGGTAAA
    ACATATGCCCCAGCATTTGATGTAGATATGAATCAGCCAGGTATACAAACTTGGTTACAA
    GAGATGAAAGATCGCTCATTAAGTAAGTATGACATTATGACTGTTGGTGAAGCGAATGGT
    GTAAGCCCTGATGATGCTGATGACTGGGTCGGGGAAGAAAATGGTAAATTTAATATGATA
    TTCCAATTTGAACATTTGGGACTGTGGAATAGTGGTGATTCTCACTTTGATGTAAATTCG
    TATAAATCTGTATTAAATAGATGGCAAAAACAACTTGAAAATAAAGGTTGGAATGCGTTG
    TTTATTGAAAATCACGACCAACCACGACGTGTATCGACGTGGGGTGACGATGACAAGTAT
    TGGTATGAATCAGCAACAAGTCATGCAGCTGTTTATTTCTTGCAACAAGGTACGCCATTC
    ATTTATCAAGGTCAAGAAATTGGTATGACGAATTATCCATTTGAAAGTATTGAAACGTTT
    AACGATGTTGCTGTTAAAAATGACTATCAAATAGTGAAAGCTCAAGGTGGAGATGTAGAC
    GCTTTACTTGCGAAATATAAAGATGAGAACCGAGATAATTCTCGCACACCAATGCAATGG
    GATGATACGTTAAATGGAGGATTTACAAATGGTGAACCGTGGTTCCCAGTGAATCCGAAT
    TATAAAACTATCAATGTTGCACAACAATTAGAAGATGAGCATTCAGTATTACAATTTTAT
    AAAGATTTAATTCAATTAAGAAAGTCTAATGATGTATACGTATATGGTCAATTTGATTTA
    GTAGATGCTGAAAATTCACAAGTTTTTGCGTACACGAGAACATTAAATGAAAAGCAAGTT
    CTTATTGTAGGTAATCTTACTAACCACGAAGCAGAATTAACTGTACCATTTGATTTAAGC
    CATGGAGAAGTAAAGCTATTTAATTATGATGCCAAAGTTAATTTAAAACAGTTACGTCCA
    TATGAAGCATGTGTTATCGAACTAAATTAA
    60
    120
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    960
    1020
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    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1650

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS07900 [old locus tag: SACOL1551 ]
  • symbol: SACOL_RS07900
  • description: alpha-glucosidase
  • length: 549
  • theoretical pI: 4.30515
  • theoretical MW: 63905.2
  • GRAVY: -0.736066

Function[edit source | edit]

  • reaction:
    EC 3.2.1.20?  ExPASy
    Alpha-glucosidaseHydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
    EC 3.2.1.1?  ExPASy
    Alpha-amylaseEndohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 597.7)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidestrehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 346.7)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesmalto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 66.1)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesmalto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 62.9)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharides1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 29.2)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 25.7)
    pullulanase, extracellular (TIGR02102; HMM-score: 24.4)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 22.2)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen debranching enzyme (TIGR01531; HMM-score: 12.8)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 12.4)
  • TheSEED:
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 380.6)
    GHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 34.7)
    Glyco_hydro_tim (CL0058) hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 17.8)
    GHD (CL0369) Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 13.5)
    DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 66
    • Cmax: 0.11
    • Cmax_pos: 63
    • Smax: 0.123
    • Smax_pos: 63
    • Smean: 0.075
    • D: 0.095
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446964655 NCBI
  • RefSeq: WP_001041911 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDFGIDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVNHTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYYFHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPEGKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGKFNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTWGDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIVKAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQLEDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTNHEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS07900 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]