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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13635 [old locus tag: SACOL2606 ]
  • pan locus tag?: SAUPAN006269000
  • symbol: SACOL_RS13635
  • pan gene symbol?: pyrD
  • synonym:
  • product: dihydroorotate dehydrogenase (quinone)

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS13635 [old locus tag: SACOL2606 ]
  • symbol: SACOL_RS13635
  • product: dihydroorotate dehydrogenase (quinone)
  • replicon: chromosome
  • strand: +
  • coordinates: 2664549..2665613
  • length: 1065
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2664549..2665613) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTACAAATTAATTAAACCTTTCTTATTCAAAATCGAACCCGAAAAAGCACACGGACTA
    ACTATCGATGCATTAAAAACGTTACAAAAGTTTCCGGTTTTATTCCCAGTCGTCGATAAA
    CTATTTACTTATAAGAATCCAACGTTATCACAAACGATACAAGGTAATACGTACGACAAT
    CCAATTGGCTTAGCAGCTGGTTTCGACAAATCTTGCGAAGTACCAAAAGCATTGGAACAC
    CTTGGATTCGGTGCTTTAGAATTAGGTGGTATCACACCTAAACCTCAACCGGGTAACCCT
    CAACCACGCATGTTTAGATTATTAGAAGATGACGCCTTGATAAATCGAATGGGCTTCAAT
    AATATTGGTATGAACAAAGCACTAAGTCATTTGCGTAAAAATGCTTATCAAGTACCTGTT
    GGTATCAATGTTGGTGTGAATAAAATGACACCTTATGAAGCGCGTTATCAAGATTATATA
    AAGGTTATTGATACGTTTAAACACGACGTTTCATTTTTCACAGTCAACATCAGTTCTCCA
    AATACTGAAAATCTTCAAAACTTCCATGATAAAGATGAATTTTCAATGTTATGCCAAGCT
    TTAACAGCATTTAAAAAACAACATGATGTAACAGTGCCAATTTATTTAAAACTAACGTCT
    GATATGGATTTCGATGGCTTAAAAGCACTATTACCAGCGATTACTGAGACATTTGACGGT
    ATCATCTTAGCAAACACAACGCGACAACGAGATGGTTTAACTTCTGCTAATAAAGTCGAA
    GAAGGCGGTTTGAGTGGTCGTCCATTATTTGAACGTAATTTAAAATTGATTAAGTATGCT
    TATCAGCAAACAAATGGTGAATTTTTAATTATAGGTACAGGCGGCGTATTCAGTACTGAA
    GATGCAATCAAAATGATGCGTCACGGTGCGTCACTTATTCAAATTTATTCATCACTTGTT
    ATTGAAGGCCCAGGTTTAACTAAGAAAATGAACAAAGGCATCGCACGTTACTTAAAAGAT
    CATCATTTTGACAATGTCAGTGATATTATAGGACTAGATGCCTAA
    60
    120
    180
    240
    300
    360
    420
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    540
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    840
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    1020
    1065

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS13635 [old locus tag: SACOL2606 ]
  • symbol: SACOL_RS13635
  • description: dihydroorotate dehydrogenase (quinone)
  • length: 354
  • theoretical pI: 8.56958
  • theoretical MW: 39536.3
  • GRAVY: -0.222599

Function[edit source | edit]

  • reaction:
    EC 1.3.98.1?  ExPASy
    Dihydroorotate oxidase (fumarate)(S)-dihydroorotate + fumarate = orotate + succinate
    EC 1.3.5.2?  ExPASy
    Dihydroorotate dehydrogenase (quinone)(S)-dihydroorotate + a quinone = orotate + a quinol
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisdihydroorotate dehydrogenase (fumarate) (TIGR01036; EC 1.3.98.1; HMM-score: 328.6)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 100.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherisopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 11)
  • TheSEED:
  • PFAM:
    TIM_barrel (CL0036) DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 244.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SACOL_RS11135(deoA)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    SACOL_RS0303550S ribosomal protein L10  [1] (data from MRSA252)
    SACOL_RS03080elongation factor Tu  [1] (data from MRSA252)
    SACOL_RS05645dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    SACOL_RS07385dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SACOL_RS07705DNA-binding protein HU  [1] (data from MRSA252)
    SACOL_RS08865aldehyde dehydrogenase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.163
    • Ymax_pos: 20
    • Cmax: 0.137
    • Cmax_pos: 56
    • Smax: 0.266
    • Smax_pos: 11
    • Smean: 0.213
    • D: 0.183
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446199560 NCBI
  • RefSeq: WP_000277415 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTYKNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQPRMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYIKVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKLTSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKLIKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGIARYLKDHHFDNVSDIIGLDA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS13635 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6

Relevant publications[edit source | edit]