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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00185
  • pan locus tag?: SAUPAN001077000
  • symbol: SAOUHSC_00185
  • pan gene symbol?: hptS
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00185
  • symbol: SAOUHSC_00185
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 202799..204355
  • length: 1557
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGACGGCATACAAACCTTATAGACATCAATTAAGACGATCGTTATTTGCGTCTACTATT
    TTTCCAGTATTTTTAGTCATTATTATTGGACTAGTAAGCTTCTATGCCATCTATATTTGG
    ATTGAGCATCGTACGATTCATCAACATGTTGATGAAAGTCAGTCATCTTTACATCATACT
    GAAAAGCAGATTCAGACATTTATTACACAGCATAATAATTCGTTTCAAGAGCTCGATTTA
    ACAAATCATCATGATGTGACCGCTACAAAACGTGAATTATTAAAATTAATACACCAACAG
    CCCGCAACACTTTATTATGAATTATCTGGACCAAATCAGTTTATAACCAATAATTATGAA
    CATTTAAACACTAAAAATATGTATTTATTTTCAACGCATCAGCTTAAATTCAAAAATAGT
    ACATATATGCTTAAAATTTATATGGCTAACACCCCACGTTTAAGTGAAATTAAGAAAGAT
    AACCGACAATTCGCACTTATCGTTGATCAATATGACAATATTTTATACGCCAACGATGAC
    CGTTTTACTATAGGTGAAAAGTATAGACCACAACAATTCGGATTTATGAATGAATCTGTA
    AAATTAAACCATGCTGACCATCGTCTCATTATTTATAAAGATATACATGAAAACATTGAA
    GATGGTATCACGTTGTTAATTGTTATGGCCGTAGTTCTTGTTTTACTAGTAATATTTGGT
    TTCATTAGCGCAGATAATATGGCTAAACGACAAACAAAAGATATCGAAACGATTATTCAA
    AAAATTTACTATGCCAAAAATCGTCATCTAGGTACATATACGCCTTTAAAAAACAACAGT
    GAACTAGAAGAAATTAATAATTATATTTACGATTTATTTGAATCAAATGAGCAACTGATC
    CATTCTATCGAGCATACAGAACGTCGCTTGCGTGATATTCAGTTAAAGGAAATAGAACGC
    CAATTCCAGCCTCATTTCCTATTCAATACGATGCAAACAATACAATATTTAATTACGTTA
    TCTCCGAAATTAGCACAAACTGTGGTCCAACAATTATCGCAAATGTTACGTTACTCATTA
    CGTACAAACTCACATACAGTGGAATTAAACGAAGAGTTGAACTACATTGAACAGTATGTT
    GCAATACAAAACATCCGTTTTGATGACATGATTAAGCTCCATATTGAAAGTTCTGAAGAA
    GCGCGTCACCAGACAATTGGTAAAATGATGCTTCAGCCACTCATAGAAAATGCTATTAAA
    CATGGTAGAGATACTGAGTCATTGGACATTACTATACGCCTGACATTGGCACGTCAGAAT
    TTACATGTTTTAGTTTGCGACAATGGTATCGGGATGTCGTCATCTCGCCTGCAATATGTA
    CGCCAATCTCTTAATAATGATGTATTTGATACTAAGCATTTGGGATTAAATCATTTACAC
    AATAAAGCAATGATTCAATATGGTTCACATGCACGACTCCATATTTTTTCAAAGCGTAAT
    CAAGGTACGTTAATTTGTTATAAAATACCACTTTCAAGGGGGAATGTAGATGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1557

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00185
  • symbol: SAOUHSC_00185
  • description: hypothetical protein
  • length: 518
  • theoretical pI: 8.26122
  • theoretical MW: 61046.4
  • GRAVY: -0.383591

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Cellular processesCellular processesSporulation and germinationsporulation integral membrane protein YtvI (TIGR02872; HMM-score: 16.6)
    Cellular processesCellular processesSporulation and germinationanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.4)
    Signal transductionRegulatory functionsProtein interactionsanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 15.4)
    Signal transductionRegulatory functionsDNA interactionstranscriptional regulator, Spx/MgsR family (TIGR01617; HMM-score: 13.9)
    and 2 more
    Cellular processesCellular processesSporulation and germinationstage II sporulation protein M (TIGR02831; HMM-score: 12.6)
    Cellular processesCellular processesCell divisioncell division protein FtsL (TIGR02209; HMM-score: 12.4)
  • TheSEED:  
    Two-component sensor histidine kinase 
  • PFAM:
    no clan definedHis_kinase; Histidine kinase (PF06580; HMM-score: 76)
    and 10 more
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 25.8)
    no clan definedSIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 16.3)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.5)
    no clan definedDUF2730; Protein of unknown function (DUF2730) (PF10805; HMM-score: 14.2)
    Tetraspannin (CL0347) Tetraspannin; Tetraspanin family (PF00335; HMM-score: 14.1)
    BPD_transp_1 (CL0404) FtsX; FtsX-like permease family (PF02687; HMM-score: 13.9)
    no clan definedSpore_III_AB; Stage III sporulation protein AB (spore_III_AB) (PF09548; HMM-score: 13.7)
    Phage_barrel (CL0504) Phage-tail_3; Putative phage tail protein (PF13550; HMM-score: 13.1)
    no clan definedDUF4477; Domain of unknown function (DUF4477) (PF14780; HMM-score: 9.5)
    STAT_int; STAT protein, protein interaction domain (PF02865; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.21
    • Ymax_pos: 11
    • Cmax: 0.118
    • Cmax_pos: 37
    • Smax: 0.492
    • Smax_pos: 1
    • Smean: 0.439
    • D: 0.299
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHRTIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPATLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKDNRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHENIEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPLKNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTIQYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIKLHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGIGMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYKIPLSRGNVDV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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