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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00186
  • pan locus tag?: SAUPAN001079000
  • symbol: SAOUHSC_00186
  • pan gene symbol?: hptA
  • synonym:
  • product: lipoprotein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00186
  • symbol: SAOUHSC_00186
  • product: lipoprotein
  • replicon: chromosome
  • strand: -
  • coordinates: 204352..205320
  • length: 969
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAAATCAAAAATTTATATCTTGCTATTATTTCTCATTTTTTTATCAGCATGCGCTAAT
    ACGCGTCACTCTGAATCCGATAAAAATGTATTAACAGTTTATTCTCCGTATCAATCAAAC
    TTGATTCGTCCAATTTTAAATGAATTTGAAAAACAAGAGCATGTCAAAATTGAAATTAAA
    CACGGATCTACTCAAGTACTGCTTTCAAACTTGCATAACGAAGATTTTTCGGAGCGTGGT
    GATGTCTTTATGGGTGGTGTGTTGTCAGAAACAATTGATCATCCAGAAGATTTTGTTCCC
    TATCAAGATACATCTGTAACACAGCAATTAGAGGATTATCGCTCGAACAATAAATATGTT
    ACTAGTTTTCTATTAATGCCAACAGTTATAGTAGTGAATTCAGATTTACAAGGAGATATT
    AAGATTCGAGGTTATCAAGATTTATTACAACCTATACTTAAAGGTAAAATTGCGTACTCA
    AATCCAAATACAACAACGACAGGCTATCAACATATGCGTGCTATTTATAGCATGCATCAT
    CGAGTAAGTGATGTGCATCAATTCCAAAACCATGCGATGCAACTGTCAAAGACGTCTAAA
    GTCATTGAAGATGTTGCAAAAGGTAAATATTACGCAGGTCTAAGCTACGAACAAGATGCA
    CGCACATGGAAAAACAAAGGTTATCCTGTATCAATCGTTTATCCAATTGAAGGAACAATG
    TTAAATGTTGATGGTATTGCTTTAGTTAAAAATGCACACCCTCATCCTAAGCGTAAAAAG
    TTAGTGCAATATTTAACAAGCCGCTCAGTACAACAACGATTAGTTGCAGAGTTCGATGCC
    AAGTCAATTCGAAAGGATGTTAGTGAACAAAGCGATCAGTCTATCGAAAATCTTAAGAAC
    ATACCTTTAATACCGAAATCTAAATTACCGGATATTCCACATCATAAATTTTTGGAGATG
    ATTCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00186
  • symbol: SAOUHSC_00186
  • description: lipoprotein
  • length: 322
  • theoretical pI: 8.99606
  • theoretical MW: 36931.9
  • GRAVY: -0.455279

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesputative 2-aminoethylphosphonate ABC transporter, periplasmic 2-aminoethylphosphonate-binding protein (TIGR03261; HMM-score: 88.7)
    2-aminoethylphosphonate ABC transporter, 2-aminoethylphosphonate binding protein (TIGR03227; HMM-score: 40.9)
    MetabolismTransport and binding proteinsOtherABC transporter periplasmic binding protein, thiB subfamily (TIGR01254; HMM-score: 27.2)
  • TheSEED:  
    Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryIron acquisition in Streptococcus Ferric iron ABC transporter, iron-binding protein 
  • PFAM:
    PBP (CL0177) SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 74.7)
    SBP_bac_6; Bacterial extracellular solute-binding protein (PF13343; HMM-score: 62.9)
    SBP_bac_8; Bacterial extracellular solute-binding protein (PF13416; HMM-score: 38.7)
    SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 23.1)
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: -0.33
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: FLSACAN
  • SignalP: Signal peptide length 25 aa
    • Ymax: 0.415
    • Ymax_pos: 25
    • Cmax: 0.26
    • Cmax_pos: 25
    • Smax: 0.838
    • Smax_pos: 16
    • Smean: 0.688
    • D: 0.522
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRPILNEFEKQEHVKIEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQDTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYSNPNTTTTGYQHMRAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQDARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVAEFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Joo Youn Park, Jong Wan Kim, Bo Youn Moon, Juyeun Lee, Ye Ji Fortin, Frank W Austin, Soo-Jin Yang, Keun Seok Seo
Characterization of a novel two-component regulatory system, HptRS, the regulator for the hexose phosphate transport system in Staphylococcus aureus.
Infect. Immun.: 2015, 83(4);1620-8
[PubMed:25644013] [WorldCat.org] [DOI] (I p)