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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00400
  • pan locus tag?: SAUPAN002130000
  • symbol: SAOUHSC_00400
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00400
  • symbol: SAOUHSC_00400
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 403207..404715
  • length: 1509
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGGGAAAATTTTAAGTTACGTAAAATGAAAGTCGGTTTAGTATCTGTTGCAATTACA
    ATGTTATATATTATGACAAACGGACAAGCAGAAGCATCTGAAAATCAAAACGCTTTAATC
    TCTAATATAAATGTAGACAATCAGGAAAAACAGAATAATGTAAATCAAGCTGTTCAGCCT
    CAAAATAATACTAATGAAACATCAAAAGTACCGGCTAATTTTGTCAAATTGAATGATATT
    AAACCAGGTGATACTTCTATACAAGGAACAACTTTACCAAATCAATTTATACTATTAACT
    ATTGATAAAAAAGATGTGAGCTCAGTTGAAGATTCTGACAGCAGCTTTGTTATGTCTGAT
    AAAGATGGGAATTTTAAGTATGACTTAAATGGTCGCAAAATTGTTCATAATCAAGAAATT
    GAAGTGTCTTCATCAGATCCCTATTTAGGTGACGATGAAGAAGATGAAGAAGTAGAAGAA
    ACTTCAACTGAAGAAGTTGGTGCTGAGGAAGAAAGTACAGAAGCTAAAGCTACATATACA
    ACACCGCGATATGAAAAAGCGTATGAAATACCGAAAGAACAGCTAAAAGAAAAAGATGGA
    CATCACCAAGTTTTTATCGAACCTATTACTGAAGGTTCAGGTATTATTAAAGGCCATACC
    TCTGTAAAAGGTAAAGTTGCTCTATCTATTAATAATAAATTTATTAACTTTGAGACAAAT
    GCTAATGGTGGTCCAAATAAAGAAGAAGCGAAATCTGGATCAGAAGGAATCTGGATGCCT
    ATTGATGACAAAGGATACTTTAATTTTGACTTCAAAACGAAACGTTTCGATGATTTAGAG
    TTAAAGAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTG
    AAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACT
    AAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTA
    CATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGAT
    AAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAATGCAGTTGTGAAGGTA
    GACTCTGAACTAGGTGAAGGTCAAGAATTCCCTGATTTGCAAGTCGATGAAAAAGGTGAA
    TTCAGCTTTGATGTAGATCATGCTGGATTTAGATTACAAAATGGAGAAACACTAAACTTC
    ACAGTAGTTGATCCTATTACAGGTGAATTATTAAGTGGAAATTTTGTTTCTAAAAACATA
    GATATTTATGAATCTCCTGAAGAAAAAGCAGATCGTGAGTTTGATGAAAGAATGGAAAAC
    ACACCTGCATATCATAAATTACATGGTGATAAAATTGTCGGCTACGATACTAACGGATTC
    CCGATTACCTGGTTTTATCCATTAGGTGAAAAGAAAGTTGAACGTAAGGCACCAAAATTA
    GAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1509

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00400
  • symbol: SAOUHSC_00400
  • description: hypothetical protein
  • length: 502
  • theoretical pI: 4.51591
  • theoretical MW: 56546.4
  • GRAVY: -0.79243

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 18.6)
  • TheSEED:  
    Hypothetical protein within Pathogenicity island SaPIn2 
  • PFAM:
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 15.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: GQAEASEN
  • SignalP: Signal peptide length 33 aa
    • Ymax: 0.818
    • Ymax_pos: 33
    • Cmax: 0.808
    • Cmax_pos: 33
    • Smax: 0.913
    • Smax_pos: 22
    • Smean: 0.83
    • D: 0.822
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNINVDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTIDKKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEETSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRFDDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPDLQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKADREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_00715 (SaeR*)
    SaeR* (TF) important in Virulence

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]