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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0408 [new locus tag: SAUSA300_RS02190 ]
  • pan locus tag?: SAUPAN002130000
  • symbol: SAUSA300_0408
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_0408 [new locus tag: SAUSA300_RS02190 ]
  • symbol: SAUSA300_0408
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 461338..462846
  • length: 1509
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAGGGAAAATTTTAAGTTACGTAAAATGAAAGTCGGTTTAGTATCTGTTGCAATTACA
    ATGTTATATATTATGACAAACGGACAAGCAGAAGCATCTGAAAATCAAAACGCTTTAATC
    TCTAATATAAATGTAGACAATCAGGAAAAACAGAATAATGTAAATCAAGCTGTTCAGCCT
    CAAAATAATACTAATGAAACATCAAAAGTACCGGCTAATTTTGTCAAATTGAATGATATT
    AAACCAGGTGATACTTCTATACAAGGAACAACTTTACCAAATCAATTTATACTATTAACT
    ATTGATAAAAAAGATGTGAGCTCAGTTGAAGATTCTGACAGCAGCTTTGTTATGTCTGAT
    AAAGATGGGAATTTTAAGTATGACTTAAATGGTCGCAAAATTGTTCATAATCAAGAAATT
    GAAGTGTCTTCATCAGATCCCTATTTAGGTGACGATGAAGAAGATGAAGAAGTAGAAGAA
    ACTTCAACTGAAGAAGTTGGTGCTGAGGAAGAAAGTACAGAAGCTAAAGCTACATATACA
    ACACCGCGATATGAAAAAGCGTATGAAATACCGAAAGAACAGCTAAAAGAAAAAGATGGA
    CATCACCAAGTTTTTATCGAACCTATTACTGAAGGTTCAGGTATTATTAAAGGCCATACC
    TCTGTAAAAGGTAAAGTTGCTCTATCTATTAATAATAAATTTATTAACTTTGAGACAAAT
    GCTAATGGTGGTCCAAATAAAGAAGAAGCGAAATCTGGATCAGAAGGAATCTGGATGCCT
    ATTGATGACAAAGGATACTTTAATTTTGACTTCAAAACGAAACGTTTCGATGATTTAGAG
    TTAAAGAAAAATGATGAGATCTCATTAACATTTGCACCTGATGACGAAGATGAGGCATTG
    AAGTCATTAATTTTCAAAACTAAGGTAACGAGTTTAGAAGATATTGATAAAGCAGAAACT
    AAATATGACCATACTAAAGTGGAAAAAGTAAAAGTATTGAAAGATGTTAAAGAAGATTTA
    CATGTAGATGAAATTTACGGAAGCTTATATCATACAGAAAAAGGTAAAGGTATTCTTGAT
    AAAGAAGGTACTAAAGTAATTAAAGGTAAGACTAAATTCGCAAATGCAGTTGTGAAGGTA
    GACTCTGAACTAGGTGAAGGTCAAGAATTCCCTGATTTGCAAGTCGATGAAAAAGGTGAA
    TTCAGCTTTGATGTAGATCATGCTGGATTTAGATTACAAAATGGAGAAACACTAAACTTC
    ACAGTAGTTGATCCTATTACAGGTGAATTATTAAGTGGAAATTTTGTTTCTAAAAACATA
    GATATTTATGAATCTCCTGAAGAAAAAGCAGATCGTGAGTTTGATGAAAGAATGGAAAAC
    ACACCTGCATATCATAAATTACATGGTGATAAAATTGTCGGCTACGATACTAACGGATTC
    CCGATTACCTGGTTTTATCCATTAGGTGAAAAGAAAGTTGAACGTAAGGCACCAAAATTA
    GAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1509

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_0408 [new locus tag: SAUSA300_RS02190 ]
  • symbol: SAUSA300_0408
  • description: hypothetical protein
  • length: 502
  • theoretical pI: 4.51591
  • theoretical MW: 56546.4
  • GRAVY: -0.79243

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 18.6)
  • TheSEED:  
    Hypothetical protein within Pathogenicity island SaPIn2 
  • PFAM:
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 15.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: GQAEASEN
  • SignalP: Signal peptide length 33 aa
    • Ymax: 0.818
    • Ymax_pos: 33
    • Cmax: 0.808
    • Cmax_pos: 33
    • Smax: 0.913
    • Smax_pos: 22
    • Smean: 0.83
    • D: 0.822
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASENQNALISNINVDNQEKQNNVNQAVQPQNNTNETSKVPANFVKLNDIKPGDTSIQGTTLPNQFILLTIDKKDVSSVEDSDSSFVMSDKDGNFKYDLNGRKIVHNQEIEVSSSDPYLGDDEEDEEVEETSTEEVGAEEESTEAKATYTTPRYEKAYEIPKEQLKEKDGHHQVFIEPITEGSGIIKGHTSVKGKVALSINNKFINFETNANGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRFDDLELKKNDEISLTFAPDDEDEALKSLIFKTKVTSLEDIDKAETKYDHTKVEKVKVLKDVKEDLHVDEIYGSLYHTEKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEGQEFPDLQVDEKGEFSFDVDHAGFRLQNGETLNFTVVDPITGELLSGNFVSKNIDIYESPEEKADREFDERMENTPAYHKLHGDKIVGYDTNGFPITWFYPLGEKKVERKAPKLEK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SaeR
    SaeR (TF) important in Virulence  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]