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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00464
  • pan locus tag?: SAUPAN002231000
  • symbol: ksgA
  • pan gene symbol?: ksgA
  • synonym:
  • product: dimethyladenosine transferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00464
  • symbol: ksgA
  • product: dimethyladenosine transferase
  • replicon: chromosome
  • strand: +
  • coordinates: 465208..466101
  • length: 894
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTTGGATAATAAAGATATTGCAACACCATCAAGAACGCGAGCGTTGTTAGATAAATAT
    GGCTTTAATTTTAAAAAAAGTTTAGGACAGAACTTTTTGATAGATGTGAATATCATTAAT
    AATATCATTGATGCAAGTGATATTGATGCACAAACTGGGGTGATTGAAATTGGTCCAGGC
    ATGGGGTCATTGACAGAACAATTGGCCAGACATGCTAAAAGAGTATTGGCATTTGAAATT
    GATCAACGTTTAATACCTGTATTAAATGATACACTATCACCTTATGATAATGTGACGGTG
    ATTAATGAAGATATTTTAAAAGCGAATATTAAAGAAGCTGTTGAAAATCATTTACAAGAT
    TGTGAAAAAATAATGGTTGTTGCAAACCTGCCGTACTATATTACGACGCCAATTTTATTA
    AATTTGATGCAACAAGATATACCAATTGATGGCTACGTGGTGATGATGCAAAAAGAAGTG
    GGCGAACGCTTAAATGCTGAAGTAGGTTCAAAAGCATATGGTTCGTTATCAATTGTCGTA
    CAATACTATACAGAGACTAGTAAAGTATTAACGGTACCTAAATCTGTATTTATGCCACCA
    CCTAATGTTGATTCAATAGTTGTAAAACTGATGCAGAGAACTGAACCGTTAGTAACAGTA
    GATAACGAGGAAGCATTCTTTAAGTTAGCAAAAGCAGCATTTGCACAAAGAAGAAAGACA
    ATTAACAATAACTATCAAAATTATTTTAAAGATGGTAAACAACACAAAGAAGTGATTTTA
    CAATGGTTGGAACAAGCAGGTATTGATCCAAGACGTCGCGGTGAAACGCTATCTATTCAA
    GATTTTGCTAAATTGTATGAAGAAAAGAAAAAATTCCCTCAATTAGAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    894

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00464
  • symbol: KsgA
  • description: dimethyladenosine transferase
  • length: 297
  • theoretical pI: 5.49072
  • theoretical MW: 33737.6
  • GRAVY: -0.280471

Function[edit source | edit]

  • reaction:
    EC 2.1.1.182?  ExPASy
    16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA = 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 281.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 18.2)
    Genetic information processingProtein synthesistRNA and rRNA base modification23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 15.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllmagnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 15.4)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 13.4)
    Unknown functionEnzymes of unknown specificityputative methylase (TIGR00537; HMM-score: 12.7)
  • TheSEED:  
    Protein MetabolismProtein biosynthesisRibosome biogenesis bacterial SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase (EC 2.1.1.182) 
  • PFAM:
    NADP_Rossmann (CL0063) RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 235.4)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 20.8)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 19.1)
    FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 17.8)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 16.4)
    Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 16)
    NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 14.6)
    RsmJ; Ribosomal RNA large subunit methyltransferase D, RlmJ (PF04378; HMM-score: 13.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.145
    • Ymax_pos: 51
    • Cmax: 0.162
    • Cmax_pos: 51
    • Smax: 0.22
    • Smax_pos: 1
    • Smean: 0.122
    • D: 0.136
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIIDASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVINEDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEVGERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLVTVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGETLSIQDFAKLYEEKKKFPQLEN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Heather C O'Farrell, Jason P Rife
Staphylococcus aureus and Escherichia coli have disparate dependences on KsgA for growth and ribosome biogenesis.
BMC Microbiol.: 2012, 12;244
[PubMed:23095113] [WorldCat.org] [DOI] (I e)