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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00461
  • pan locus tag?: SAUPAN002227000
  • symbol: SAOUHSC_00461
  • pan gene symbol?: metS
  • synonym:
  • product: methionyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00461
  • symbol: SAOUHSC_00461
  • product: methionyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 461717..463690
  • length: 1974
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCTAAAGAAACATTTTATATAACAACCCCAATATACTATCCTAGTGGGAATTTACAT
    ATAGGACATGCATATTCTACAGTGGCTGGAGATGTTATTGCAAGATATAAGAGAATGCAA
    GGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAA
    GCTCAAAAAGCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAA
    CAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAACAACTGAAGAACGT
    CATAAACATGTCGTTGAGCAAGTGTTTGAACGTTTATTAAAGCAAGGTGATATCTATTTA
    GGTGAATATGAAGGTTGGTATTCTGTTCCGGATGAAACATACTATACAGAGTCACAATTA
    GTAGACCCACAATACGAAAACGGTAAAATTATTGGTGGCAAAAGTCCAGATTCTGGACAC
    GAAGTTGAACTAGTTAAAGAAGAAAGTTATTTCTTTAATATTAGTAAATATACAGACCGT
    TTATTAGAGTTCTATGACCAAAATCCAGATTTTATACAACCACCATCAAGAAAAAATGAA
    ATGATTAACAACTTCATTAAACCAGGACTTGCTGATTTAGCTGTTTCTCGTACATCATTT
    AACTGGGGTGTCCATGTTCCGTCTAATCCAAAACATGTTGTTTATGTTTGGATTGATGCG
    TTAGTTAACTATATTTCAGCATTAGGCTATTTATCAGATGATGAGTCACTATTTAACAAA
    TACTGGCCAGCAGATATTCATTTAATGGCTAAGGAAATTGTGCGATTCCACTCAATTATT
    TGGCCTATTTTATTGATGGCATTAGACTTACCGTTACCTAAAAAAGTCTTTGCACATGGT
    TGGATTTTGATGAAAGATGGAAAAATGAGTAAATCTAAAGGTAATGTCGTAGACCCTAAT
    ATTTTAATTGATCGCTATGGTTTAGATGCTACACGTTATTATCTAATGCGTGAATTACCA
    TTTGGTTCAGATGGCGTATTTACACCTGAAGCATTTGTTGAGCGTACAAATTTCGATCTA
    GCAAATGACTTAGGTAACTTAGTAAACCGTACGATTTCTATGGTTAATAAGTACTTTGAT
    GGCGAATTACCAGCGTATCAAGGTCCACTTCATGAATTAGATGAAGAAATGGAAGCTATG
    GCTTTAGAAACAGTGAAAAGCTACACTGAAAGCATGGAAAGTTTGCAATTTTCTGTGGCA
    TTATCTACGGTATGGAAGTTTATTAGTAGAACGAATAAGTATATTGACGAAACAACGCCT
    TGGGTATTAGCTAAGGACGATAGCCAAAAAGATATGTTAGGCAATGTAATGGCTCACTTA
    GTTGAAAATATTCGTTATGCAGCTGTATTATTACGTCCATTCTTAACACATGCGCCGAAA
    GAGATTTTTGAACAATTGAACATTAACAATCCTCAATTTATGGAATTTAGTAGTTTAGAG
    CAATATGGTGTGCTTAATGAGTCAATTATGGTTACTGGGCAACCTAAACCTATTTTCCCA
    AGATTGGATAGCGAAGCGGAAATTGCATATATCAAAGAATCAATGCAACCGCCTGCTACT
    AAAGAGGAAAAAGAAGAGATTCCTAGCAAACCTCAAATTGATATTAAAGACTTTGATAAA
    GTTGAAATTAAGGCAGCAACGATTATTGATGCTGAACATGTTAAGAAGTCAGATAAGCTT
    TTAAAAATTCAAGTAGACTTAGATTCTGAACAAAGACAAATTGTATCAGGAATTGCCAAA
    TTCTATACACCAGATGATATTATTGGTAAAAAAGTAGCAGTTGTTACTAACCTGAAACCA
    GCTAAATTAATGGGACAAAAATCTGAAGGTATGATATTATCTGCTGAAAAAGATGGTGTA
    TTAACCTTAGTAAGTTTACCAAGTGCAATTCCAAATGGTGCAGTGATTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1974

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00461
  • symbol: SAOUHSC_00461
  • description: methionyl-tRNA synthetase
  • length: 657
  • theoretical pI: 4.94244
  • theoretical MW: 74885
  • GRAVY: -0.338661

Function[edit source | edit]

  • reaction:
    EC 6.1.1.10?  ExPASy
    Methionine--tRNA ligaseATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationmethionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 564.6)
    Genetic information processingProtein synthesistRNA aminoacylationvaline--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 149)
    Genetic information processingProtein synthesistRNA aminoacylationmethionine--tRNA ligase, beta subunit (TIGR00399; EC 6.1.1.10; HMM-score: 138.6)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 114.5)
    Genetic information processingProtein synthesistRNA aminoacylationisoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 112.1)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 81.2)
    Genetic information processingProtein synthesistRNA aminoacylationcysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 62)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingexport-related chaperone protein CsaA (TIGR02222; HMM-score: 38.7)
    Genetic information processingProtein fateProtein folding and stabilizationexport-related chaperone protein CsaA (TIGR02222; HMM-score: 38.7)
    Genetic information processingProtein synthesistRNA aminoacylationphenylalanine--tRNA ligase, beta subunit (TIGR00472; EC 6.1.1.20; HMM-score: 27.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogscysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 26.2)
    Genetic information processingProtein synthesistRNA aminoacylationarginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 23.8)
    Genetic information processingProtein synthesistRNA aminoacylationlysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 16.4)
  • TheSEED:  
    Methionyl-tRNA synthetase (EC 6.1.1.10) 
  • PFAM:
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 387.2)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 105.5)
    OB (CL0021) tRNA_bind; Putative tRNA binding domain (PF01588; HMM-score: 89.1)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 56.5)
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 36.3)
    HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 25.3)
    Pilus (CL0327) PilM; PilM (PF07419; HMM-score: 14.6)
    no clan definedARL2_Bind_BART; The ARF-like 2 binding protein BART (PF11527; HMM-score: 12.9)
    HUP (CL0039) tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 12.6)
    no clan definedT4_neck-protein; Virus neck protein (PF11649; HMM-score: 10.8)
    HUP (CL0039) tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 9.9)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 25
    • Cmax: 0.19
    • Cmax_pos: 35
    • Smax: 0.15
    • Smax_pos: 23
    • Smean: 0.093
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGHEVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. 2.000 2.001 2.002 2.003 2.004 2.005 2.006 2.007 2.008 2.009 2.010 2.011 2.012 2.013 2.014 2.015 2.016 2.017 2.018 2.019 2.020 2.021 2.022 2.023 2.024 2.025 2.026 2.027 2.028 2.029 2.030 2.031 2.032 2.033 2.034 2.035 2.036 2.037 2.038 2.039 2.040 2.041 2.042 2.043 2.044 2.045 2.046 2.047 2.048 2.049 2.050 2.051 2.052 2.053 2.054 2.055 2.056 2.057 2.058 2.059 2.060 2.061 2.062 2.063 2.064 2.065 2.066 2.067 2.068 2.069 2.070 2.071 2.072 2.073 2.074 2.075 2.076 2.077 2.078 2.079 2.080 2.081 2.082 2.083 2.084 2.085 2.086 2.087 2.088 2.089 2.090 2.091 2.092 2.093 2.094 2.095 2.096 2.097 2.098 2.099 2.100 2.101 2.102 2.103 2.104 2.105 2.106 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]