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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01485
  • pan locus tag?: SAUPAN003929000
  • symbol: ndk
  • pan gene symbol?: ndk
  • synonym:
  • product: nucleoside diphosphate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01485
  • symbol: ndk
  • product: nucleoside diphosphate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1442371..1442802
  • length: 432
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGATTAAACCAGATGCAGTACAAAGAAATCTAATTGGTGAAGTAATTTCAAGAATTGAA
    AGAAAAGGACTAAAACTTGTCGGTGGTAAATTAATGCAAGTACCAATGGAACTTGCTGAA
    ACACATTATGGTGAACACCAAGGTAAACCATTTTATAATGATTTAATTTCATTTATTACA
    TCAGCACCAGTGTTCGCAATGGTAGTTGAAGGTGAAGATGCAGTTAATGTATCTAGACAT
    ATTATTGGCAGCACCAATCCTTCAGAAGCTTCACCAGGATCAATTAGAGGTGATTTAGGT
    TTAACTGTTGGTAGAAATATCATTCACGGTTCAGATTCATTAGAGTCTGCTGAACGTGAA
    ATTAATCTATGGTTTAATGAAAATGAAATTACTAGCTATGCTTCACCACGTGATGCATGG
    TTATATGAATAA
    60
    120
    180
    240
    300
    360
    420
    432

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01485
  • symbol: Ndk
  • description: nucleoside diphosphate kinase
  • length: 143
  • theoretical pI: 4.79684
  • theoretical MW: 15796.7
  • GRAVY: -0.257343

Function[edit source | edit]

  • reaction:
    EC 2.7.4.6?  ExPASy
    Nucleoside-diphosphate kinaseATP + nucleoside diphosphate = ADP + nucleoside triphosphate
  • TIGRFAM:
  • TheSEED:  
    Nucleosides and NucleotidesPurinesPurine conversions Nucleoside diphosphate kinase (EC 2.7.4.6) 
    Pyrimidinespyrimidine conversions Nucleoside diphosphate kinase (EC 2.7.4.6) 
  • PFAM:
    no clan definedNDK; Nucleoside diphosphate kinase (PF00334; HMM-score: 183.1)
    TIM_barrel (CL0036) OMPdecase; Orotidine 5'-phosphate decarboxylase / HUMPS family (PF00215; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.167
    • Ymax_pos: 40
    • Cmax: 0.164
    • Cmax_pos: 40
    • Smax: 0.308
    • Smax_pos: 38
    • Smean: 0.124
    • D: 0.15
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMELAETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIRGDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]