From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01486
  • pan locus tag?: SAUPAN003930000
  • symbol: SAOUHSC_01486
  • pan gene symbol?: hepT
  • synonym:
  • product: heptaprenyl diphosphate syntase component II

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01486
  • symbol: SAOUHSC_01486
  • product: heptaprenyl diphosphate syntase component II
  • replicon: chromosome
  • strand: -
  • coordinates: 1442912..1443856
  • length: 945
  • essential: no [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAACAATGAAATTAAGAAAGTGGAACAACGACTTGAAAAGGCAATAAAAAGTAAAGAT
    TCTGTATTAGAACAGGCATCATTACACTTATTGTCTTCTGGTGGTAAAAGAGTACGCCCA
    GCATTTGTTATTCTGAGTAGCCAATTTGGCAAAGATGAACAAACGTCTGAACAAACGTAT
    CAAGTTGCAGTCGCATTAGAGTTAATTCATATGGCAACACTTGTTCATGATGACGTTATT
    GATAAAAGCGACAAGCGTCGAGGCAAGTTAACCATATCAAAGAAATGGGATCAGACAACT
    GCTATTTTAACTGGGAATTTTTTATTGGCATTAGGACTTGAACACTTAATGGCCGTTAAA
    GATAATCGTGTACATCAATTGATATCTGAATCTATCGTTGATGTTTGTAGAGGGGAACTT
    TTCCAATTTCAAGACCAATTTAACAGTCAACAGACAATTATTAATTATTTACGACGTATC
    AATCGCAAAACAGCACTGTTAATTCAAATATCAACTGAAGTTGGTGCAATTACTTCTCAA
    TCTGATAAAGAGACTGTACGAAAATTGAAAATGATTGGTCATTATATAGGTATGAGCTTC
    CAAATCATTGATGATGTATTAGACTTCACAAGTACCGAAAAGAAATTAGGTAAGCCGGTC
    GGAAGTGATTTGCTTAATGGTCATATTACGTTACCGATTTTATTAGAAATGCGTAAAAAT
    CCAGACTTCAAATTGAAAATCGAACAGTTACGTCGTGATAGTGAACGCAAAGAATTTGAA
    GAATGTATCCAAATCATTAGAAAATCTGACAGCATCGATGAGGCTAAGGCAGTAAGTTCG
    AAGTATTTAAGTAAAGCTTTGAATTTGATTTCTGAGTTACCAGATGGACATCCGAAATCA
    CTACTTTTAAGTTTGACGAAAAAAATGGGTTCAAGAAACACGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01486
  • symbol: SAOUHSC_01486
  • description: heptaprenyl diphosphate syntase component II
  • length: 314
  • theoretical pI: 9.79656
  • theoretical MW: 35584.9
  • GRAVY: -0.357325

Function[edit source | edit]

  • reaction:
    EC 2.5.1.30?  ExPASy
    Heptaprenyl diphosphate synthase(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneheptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 377.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonesolanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 242.2)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsIsoprenoid scratch Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    IsoprenoidsIsoprenoinds for Quinones Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    IsoprenoidsPolyprenyl Diphosphate Biosynthesis Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
  • PFAM:
    Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 222.8)
    HEPPP_synt_1; Heptaprenyl diphosphate synthase (HEPPP synthase) subunit 1 (PF07307; HMM-score: 29.4)
    no clan definedBaculo_p33; Baculovirus P33 (PF05214; HMM-score: 16.5)
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.145
    • Ymax_pos: 12
    • Cmax: 0.145
    • Cmax_pos: 56
    • Smax: 0.23
    • Smax_pos: 1
    • Smean: 0.194
    • D: 0.164
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVRPAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWDQTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIINYLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTEKKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDSIDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]