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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1510 [new locus tag: SACOL_RS07685 ]
  • pan locus tag?: SAUPAN003930000
  • symbol: SACOL1510
  • pan gene symbol?: hepT
  • synonym:
  • product: polyprenyl synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1510 [new locus tag: SACOL_RS07685 ]
  • symbol: SACOL1510
  • product: polyprenyl synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1549577..1550536
  • length: 960
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    GTGGCAAAGTTAAACATGAACAATGAAATTAAGAAAGTGGAACAACGACTTGAAAAGGCA
    ATAAAAAGTAAAGATTCTGTATTAGAACAGGCATCATTACACTTATTGTCTTCTGGTGGT
    AAAAGAGTACGCCCAGCATTTGTTATTCTGAGTAGCCAATTTGGCAAAGATGAACAAACG
    TCTGAACAAACGTATCAAGTTGCAGTCGCATTAGAGTTAATTCATATGGCAACACTTGTT
    CATGATGACGTTATTGATAAAAGCGACAAGCGTCGAGGCAAGTTAACCATATCAAAGAAA
    TGGGATCAGACAACTGCTATTTTAACTGGGAATTTTTTATTGGCATTAGGACTTGAACAC
    TTAATGGCCGTTAAAGATAATCGTGTACATCAATTGATATCTGAATCTATCGTTGATGTT
    TGTAGAGGGGAACTTTTCCAATTTCAAGACCAATTTAACAGTCAACAGACAATTATTAAT
    TATTTACGACGTATCAATCGCAAAACAGCACTGTTAATTCAAATATCAACTGAAGTTGGT
    GCAATTACTTCTCAATCTGATAAAGAGACTGTACGAAAATTGAAAATGATTGGTCATTAT
    ATAGGTATGAGCTTCCAAATCATTGATGATGTATTAGACTTCACAAGTACCGAAAAGAAA
    TTAGGTAAGCCGGTCGGAAGTGATTTGCTTAATGGTCATATTACGTTACCGATTTTATTA
    GAAATGCGTAAAAATCCAGACTTCAAATTGAAAATCGAACAGTTACGTCGTGATAGTGAA
    CGCAAAGAATTTGAAGAATGTATCCAAATCATTAGAAAATCTGACAGCATCGATGAGGCT
    AAGGCAGTAAGTTCGAAGTATTTAAGTAAAGCTTTGAATTTGATTTCTGAGTTACCAGAT
    GGACATCCGAAATCACTACTTTTAAGTTTGACGAAAAAAATGGGTTCAAGAAACACGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1510 [new locus tag: SACOL_RS07685 ]
  • symbol: SACOL1510
  • description: polyprenyl synthetase
  • length: 319
  • theoretical pI: 9.89584
  • theoretical MW: 36142.6
  • GRAVY: -0.351411

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneheptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 378.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonesolanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 242.5)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsIsoprenoid scratch Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    IsoprenoidsIsoprenoinds for Quinones Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    IsoprenoidsPolyprenyl Diphosphate Biosynthesis Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
  • PFAM:
    Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 222.7)
    HEPPP_synt_1; Heptaprenyl diphosphate synthase (HEPPP synthase) subunit 1 (PF07307; HMM-score: 29.4)
    no clan definedBaculo_p33; Baculovirus P33 (PF05214; HMM-score: 16.5)
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.161
    • Ymax_pos: 17
    • Cmax: 0.139
    • Cmax_pos: 61
    • Smax: 0.312
    • Smax_pos: 10
    • Smean: 0.24
    • D: 0.192
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVRPAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWDQTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIINYLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTEKKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDSIDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 47 [5]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]