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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1359 [new locus tag: SAUSA300_RS07410 ]
  • pan locus tag?: SAUPAN003930000
  • symbol: SAUSA300_1359
  • pan gene symbol?: hepT
  • synonym:
  • product: polyprenyl synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1359 [new locus tag: SAUSA300_RS07410 ]
  • symbol: SAUSA300_1359
  • product: polyprenyl synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1526290..1527234
  • length: 945
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAACAATGAAATTAAGAAAGTGGAACAACGACTTGAAAAGGCAATAAAAAGTAAAGAT
    TCTGTATTAGAACAGGCATCATTACACTTATTGTCTTCTGGTGGTAAAAGAGTACGCCCA
    GCATTTGTTATTCTGAGTAGCCAATTTGGCAAAGATGAACAAACGTCTGAACAAACGTAT
    CAAGTTGCAGTCGCATTAGAGTTAATTCATATGGCAACACTTGTTCATGATGACGTTATT
    GATAAAAGCGACAAGCGTCGAGGCAAGTTAACCATATCAAAGAAATGGGATCAGACAACT
    GCTATTTTAACTGGGAATTTTTTATTGGCATTAGGACTTGAACACTTAATGGCCGTTAAA
    GATAATCGTGTACATCAATTGATATCTGAATCTATCGTTGATGTTTGTAGAGGGGAACTT
    TTCCAATTTCAAGACCAATTTAACAGTCAACAGACAATTATTAATTATTTACGACGTATC
    AATCGCAAAACAGCACTGTTAATTCAAATATCAACTGAAGTTGGTGCAATTACTTCTCAA
    TCTGATAAAGAGACTGTACGAAAATTGAAAATGATTGGTCATTATATAGGTATGAGCTTC
    CAAATCATTGATGATGTATTAGACTTCACAAGTACCGAAAAGAAATTAGGTAAGCCGGTC
    GGAAGTGATTTGCTTAATGGTCATATTACGTTACCGATTTTATTAGAAATGCGTAAAAAT
    CCAGACTTCAAATTGAAAATCGAACAGTTACGTCGTGATAGTGAACGCAAAGAATTTGAA
    GAATGTATCCAAATCATTAGAAAATCTGACAGCATCGATGAGGCTAAGGCAGTAAGTTCG
    AAGTATTTAAGTAAAGCTTTGAATTTGATTTCTGAGTTACCAGATGGACATCCGAAATCA
    CTACTTTTAAGTTTGACGAAAAAAATGGGTTCAAGAAACACGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

Protein[edit source | edit]

Protein Data Bank: 5H9D

General[edit source | edit]

  • locus tag: SAUSA300_1359 [new locus tag: SAUSA300_RS07410 ]
  • symbol: SAUSA300_1359
  • description: polyprenyl synthetase
  • length: 314
  • theoretical pI: 9.79656
  • theoretical MW: 35584.9
  • GRAVY: -0.357325

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneheptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 377.5)
    and 1 more
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonesolanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 242.2)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsIsoprenoid scratch Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    and 3 more
    IsoprenoidsIsoprenoinds for Quinones Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    IsoprenoidsPolyprenyl Diphosphate Biosynthesis Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
    Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Heptaprenyl diphosphate synthase component II (EC 2.5.1.30) 
  • PFAM:
    Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 222.8)
    and 3 more
    HEPPP_synt_1; Heptaprenyl diphosphate synthase (HEPPP synthase) subunit 1 (PF07307; HMM-score: 29.4)
    no clan definedBaculo_p33; Baculovirus P33 (PF05214; HMM-score: 16.5)
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 15.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.145
    • Ymax_pos: 12
    • Cmax: 0.145
    • Cmax_pos: 56
    • Smax: 0.23
    • Smax_pos: 1
    • Smean: 0.194
    • D: 0.164
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVRPAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWDQTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIINYLRRINRKTALLIQISTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTEKKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSERKEFEECIQIIRKSDSIDEAKAVSSKYLSKALNLISELPDGHPKSLLLSLTKKMGSRNT

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]