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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01047
  • pan locus tag?: SAUPAN003324000
  • symbol: SAOUHSC_01047
  • pan gene symbol?: potB
  • synonym:
  • product: spermidine/putrescine ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01047
  • symbol: SAOUHSC_01047
  • product: spermidine/putrescine ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 1015519..1016316
  • length: 798
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCGTAATACTAATAAATTTCTCTTAATCCCGTATTTACTATGGATGGTTATATTTATT
    ATTGTACCAGTTGTATTACTCATTTATTTTTCATTTTTAGATATCAATGGACATTTTAGT
    TTCACGAATTATCAACAAATTTTTACTACAAAATATTTGAAAATGTTTGCATATTCAATT
    TTATATGCCGCTTCAATAACAATTATTACCTTGACTATCAGTTATCCAGCTGCCTATTAT
    ATTACTCGTTCGAAATTTCAAAATATCTTATTAATGATAATGATTATTCCAACATGGATA
    AATTTATTGTTAAAGACATATGCTTTTATAGGTTTATTAAGTCATGATGGCGTGATTAAT
    CAATTTTTCCACTTATTTAATTTACCATCATTCAATTTGTTATTTACAACTGGTGCGTTT
    TTAGTAGTGGCAAGTTACATTTATATACCATTTATGATTTTACCTATATTTAATAGCATG
    AAAGCAATTCCTAATAATTTATTGCAGGCCTCAAGTGATTTAGGTGCCAGTCCTTTCTAT
    ACTTTTAGAAAAGTAATCATGCCGTTAACAAAAGAAGGTGTTATGACTGGGATTCAAGTA
    ACCTTTATTCCATCACTTTCATTATTTATGATTACAAGATTAATTGCAGGTAATAAAGTC
    ATAAATATAGGTACGGCAATAGAGGAACAATTTTTAACAATTCAAAATTATGGTATGGGA
    TCAACTATAGCTATATTCTTAATTGTATTTATGGCATTCATTTTAATCATTACAAAATCA
    TCTAATGGGAGAGGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    798

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01047
  • symbol: SAOUHSC_01047
  • description: spermidine/putrescine ABC transporter permease
  • length: 265
  • theoretical pI: 10.0419
  • theoretical MW: 30150
  • GRAVY: 0.869434

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesputative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 77)
    MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 64.6)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 64.1)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein (TIGR00969; HMM-score: 54)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 49.6)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 39.3)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 31.1)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 17.5)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 15)
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 14.2)
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 13)
    MetabolismTransport and binding proteinsOtherthiamine/thiamine pyrophosphate ABC transporter, permease protein (TIGR01253; HMM-score: 9.8)
  • TheSEED:  
    Amino Acids and DerivativesArginine; urea cycle, polyaminesPolyamine Metabolism Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) 
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 40.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.153
    • Ymax_pos: 11
    • Cmax: 0.142
    • Cmax_pos: 41
    • Smax: 0.283
    • Smax_pos: 6
    • Smean: 0.231
    • D: 0.183
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNYQQIFTTKYLKMFAYSILYAASITIITLTISYPAAYYITRSKFQNILLMIMIIPTWINLLLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSMKAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKSSNGRG

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]