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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01643
  • pan locus tag?: SAUPAN004123000
  • symbol: SAOUHSC_01643
  • pan gene symbol?: comGA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01643
  • symbol: SAOUHSC_01643
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1559316..1560290
  • length: 975
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTA
    CATTTTATTCCAGTTAAAAATGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAG
    CAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTATTAGTTTATATGAAGTTTCAA
    GCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
    AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGT
    GTTATCAGAATTGTACCTCAATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGAT
    TTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGTTTAGTGGACCAACTGGTTCA
    GGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
    GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTG
    AATGATAAAGCTGGCATAAACTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCT
    GATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTGCCAAGTGTGTTATACAGGCT
    AGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
    TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATT
    ATAAACCAACGACTTGTAACTACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTA
    TCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATTCATTACCATCATCATTTAAG
    AACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
    CATAAATACATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01643
  • symbol: SAOUHSC_01643
  • description: hypothetical protein
  • length: 324
  • theoretical pI: 9.07987
  • theoretical MW: 36864.8
  • GRAVY: -0.0317901

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesPathogenesistype II secretion system protein E (TIGR02533; HMM-score: 221.8)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype II secretion system protein E (TIGR02533; HMM-score: 221.8)
    Cell structureCell envelopeSurface structurestype IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 213.5)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype IV-A pilus assembly ATPase PilB (TIGR02538; HMM-score: 213.5)
    Cell structureCell envelopeSurface structurestwitching motility protein (TIGR01420; HMM-score: 146.5)
    Cellular processesCellular processesChemotaxis and motilitytwitching motility protein (TIGR01420; HMM-score: 146.5)
    Genetic information processingMobile and extrachromosomal element functionsPlasmid functionsplasmid transfer ATPase TraJ (TIGR02525; HMM-score: 84.9)
    Dot/Icm secretion system ATPase DotB (TIGR02524; HMM-score: 58.2)
    Cellular processesCellular processesConjugationP-type conjugative transfer ATPase TrbB (TIGR02782; HMM-score: 57.5)
    P-type DNA transfer ATPase VirB11 (TIGR02788; HMM-score: 54.5)
    helicase/secretion neighborhood ATPase (TIGR03819; HMM-score: 35.9)
    Cellular processesCellular processesSporulation and germinationstage III sporulation protein AA (TIGR02858; HMM-score: 17.1)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 14.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDnaA regulatory inactivator Hda (TIGR03420; HMM-score: 14.4)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative secretion ATPase, PEP-CTERM locus subfamily (TIGR03015; HMM-score: 14.1)
    Cellular processesCellular processesChemotaxis and motilityflagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 12.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsguanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 12.8)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 12.2)
  • TheSEED:  
    DNA MetabolismDNA uptake, competenceGram Positive Competence Late competence protein ComGA, access of DNA to ComEA 
    DNA uptake, competenceLate competence Late competence protein ComGA, access of DNA to ComEA 
  • PFAM:
    P-loop_NTPase (CL0023) T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 192.4)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 26.7)
    DAP3; Mitochondrial ribosomal death-associated protein 3 (PF10236; HMM-score: 18)
    AAA_23; AAA domain (PF13476; HMM-score: 17.7)
    AAA_22; AAA domain (PF13401; HMM-score: 15.7)
    Rad17; Rad17 cell cycle checkpoint protein (PF03215; HMM-score: 15.4)
    ATP_bind_1; Conserved hypothetical ATP binding protein (PF03029; HMM-score: 15.4)
    AAA_30; AAA domain (PF13604; HMM-score: 15.2)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 15.2)
    AAA_14; AAA domain (PF13173; HMM-score: 14.8)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14.6)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 14.4)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 14.4)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.4)
    AAA_33; AAA domain (PF13671; HMM-score: 12.6)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 12.3)
    AAA_25; AAA domain (PF13481; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.89
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 18
    • Cmax: 0.12
    • Cmax_pos: 23
    • Smax: 0.215
    • Smax_pos: 11
    • Smean: 0.151
    • D: 0.142
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLFNKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLNVISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMHKYI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]