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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01679
  • pan locus tag?: SAUPAN004159000
  • symbol: SAOUHSC_01679
  • pan gene symbol?: mtaB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01679
  • symbol: SAOUHSC_01679
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1588289..1589635
  • length: 1347
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCAACAGTTGCGTTTCACACATTAGGTTGTAAAGTAAACCATTATGAAACTGAAGCA
    ATCTGGCAATTATTTAAAGAAGCAAACTATGAGCGCGTTGACTTTGAAGCGAATGCTGAT
    GTATTTGTTATTAATACTTGTACAGTAACGAATACGGGTGATAAAAAAAGTCGTCAAATA
    ATTAGACGTGCAATAAGACAAAATCCTGATGCTGTAATCTGTGTAACAGGTTGTTATGCG
    CAAACTTCATCAGCTGAAATTATGGAAATTCCTGGTGTCGATGTAGTAGTTGGTACACAA
    GATAGACATAAACTATTAGGTTACATTGACGAATTCCGTAAAGAACGCCAACCAATTAAT
    GGTGTTGGAAATATCATGAAAAATCGTAAATATGAAGAATTAGATGTCCCTTATTTTACA
    GATAGAACACGTGCGTCATTAAAAATTCAAGAAGGTTGTAACAACTTCTGCACATTCTGT
    ATTATTCCATGGGCTCGTGGCTTAATGCGTTCAAGAGATCCGGAAAAAGTAGTTGAACAA
    GCGACGCAACTAGTGAATTCAGGATATAAGGAAATTGTATTGACGGGAATTCATACAGGT
    GGATATGGTCAAGATTTAAAAGATTATAACTTGGCCCAATTATTACGTGATCTTGAAACG
    ATTAATGGATTAGAACGAATTCGAATTTCTTCAATTGAAGCAAGTCAACTTACAGATGAA
    GTAATTGACGTTTTAGAACGTTCAACAAAAGTTGTGCGTCATTTGCATATTCCATTACAA
    TCTGGTTCAGATACAGTATTAAAACGTATGAGACGTAAGTATACAATGGATAGATTTTCA
    GAACGATTAACAAAATTGCATAAAGCTTTACCAGACTTGGCAGTTACGAGTGATGTAATT
    GTTGGTTTCCCAGGTGAAACTGAAGCTGAGTTCCAAGAAACATATGATTTTATCGTAAAG
    CATAAGTTCTCTGAACTGCATGTTTTCCCTTATTCTCCTAGAATTGGCACGCCAGCTGCA
    AGAATGGATGACCAAATTGATGAAGAAATTAAAAATGAACGGGTGCATAAGTTAATTACG
    CTAAGCAATCAACTCGGAAAGTTATATGCGTCTAAATTTGATCAAGATGTGCTTGAAGTA
    ATTCCTGAGGAACAGGGTGACACAGAAGGTACATTAGTTGGATATGCAGATAATTATATG
    AAAGTACAATTTGAAGGTGACGAATCACTCATAGGTCAAATTGTAAAAGTTAAAATTACG
    CAAGCAAATTATCCATTAAATGAAGGGCAAGCAATTAAAGTTGTTGATTTCGCAACAAAT
    AAATCTGATAGAGAAGTTTTAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01679
  • symbol: SAOUHSC_01679
  • description: hypothetical protein
  • length: 448
  • theoretical pI: 5.53724
  • theoretical MW: 50955.5
  • GRAVY: -0.397321

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 494.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationMiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 476.1)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 354.1)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein S12 methylthiotransferase RimO (TIGR01125; EC 2.1.1.-,2.8.1.-; HMM-score: 295.7)
    Genetic information processingProtein synthesistRNA and rRNA base modificationMiaB-like tRNA modifying enzyme, archaeal-type (TIGR01578; HMM-score: 276.4)
    Unknown functionEnzymes of unknown specificityB12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 95.7)
    Unknown functionEnzymes of unknown specificityB12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 82.8)
    Cellular processesCellular processesToxin production and resistanceradical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 52.1)
    hopanoid biosynthesis associated radical SAM protein HpnJ (TIGR03471; HMM-score: 39.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamincoproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 33.3)
    Cellular processesCellular processesAdaptations to atypical conditionsKamA family protein (TIGR00238; HMM-score: 29.6)
    Unknown functionEnzymes of unknown specificityuncharacterized radical SAM protein YgiQ (TIGR03904; HMM-score: 29.3)
    Unknown functionEnzymes of unknown specificityradical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 27.6)
    Unknown functionEnzymes of unknown specificityradical SAM family uncharacterized protein (TIGR03960; HMM-score: 25.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersLipoatelipoyl synthase (TIGR00510; EC 2.8.1.8; HMM-score: 24.1)
    magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (TIGR02026; HMM-score: 20.8)
    Cellular processesCellular processesToxin production and resistancetryptophan 2-C-methyltransferase (TIGR04428; EC 2.1.1.106; HMM-score: 19.9)
    Cellular processesCellular processesAdaptations to atypical conditionsglutamate 2,3-aminomutase (TIGR04368; EC 5.4.3.9; HMM-score: 19.8)
    putative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 19)
    lysine-2,3-aminomutase-related protein (TIGR03822; EC 5.4.3.-; HMM-score: 15.7)
    His-Xaa-Ser system radical SAM maturase HxsC (TIGR03977; HMM-score: 15.3)
    Genetic information processingProtein fateProtein modification and repairEF-P beta-lysylation protein EpmB (TIGR03821; EC 5.4.3.-; HMM-score: 15.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminoxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 13.4)
    lysine-2,3-aminomutase (TIGR03820; EC 5.4.3.2; HMM-score: 13.4)
    B12-binding domain/radical SAM domain protein, rhizo-twelve system (TIGR04295; HMM-score: 11.3)
  • TheSEED:  
    Protein MetabolismProtein processing and modificationRibosomal protein S12p Asp methylthiotransferase tRNA-t(6)A37 methylthiotransferase 
    RNA MetabolismRNA processing and modificationMethylthiotransferases tRNA-t(6)A37 methylthiotransferase 
    Stress ResponseHeat shockHeat shock dnaK gene cluster extended tRNA-t(6)A37 methylthiotransferase 
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 102.8)
    CheY (CL0304) UPF0004; Uncharacterized protein family UPF0004 (PF00919; HMM-score: 92.2)
    OB (CL0021) TRAM; TRAM domain (PF01938; HMM-score: 27.7)
    NADP_Rossmann (CL0063) B12-binding; B12 binding domain (PF02310; HMM-score: 18.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.134
    • Ymax_pos: 21
    • Cmax: 0.13
    • Cmax_pos: 21
    • Smax: 0.218
    • Smax_pos: 1
    • Smean: 0.138
    • D: 0.136
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVINTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDRHKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFCIIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDLETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTMDRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPRIGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTLVGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]