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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01791
  • pan locus tag?: SAUPAN004306000
  • symbol: SAOUHSC_01791
  • pan gene symbol?: dnaI
  • synonym:
  • product: primosomal protein DnaI

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01791
  • symbol: SAOUHSC_01791
  • product: primosomal protein DnaI
  • replicon: chromosome
  • strand: -
  • coordinates: 1688106..1689026
  • length: 921
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAGCAATTTAAAAGTATAATTAACACGTCGCAGGACTTTGAAAAAAGAATAGAAAAG
    ATAAAAAAAGAAGTAATCAATGACCCAGATGTTAAGCAATTTTTGGAAGCGCATCGAGCT
    GAATTAACGAATGCTATGATTGATGAAGACTTAAATGTGTTACAAGAGTATAAAGATCAA
    CAAAAACATTATGACGGTCATAAATTTGCTGATTGTCCAAATTTCGTAAAGGGGCATGTG
    CCTGAGTTATATGTTGATAATAACCGAATTAAAATACGCTATTTACAATGCCCATGTAAA
    ATCAAGTACGACGAAGAACGCTTTGAAGCTGAGCTAATTACATCTCATCATATGCAACGA
    GATACTTTAAATGCCAAATTGAAAGATATTTATATGAATCATCGAGACCGTCTTGATGTA
    GCTATGGCAGCAGATGATATTTGTACAGCAATAACTAATGGGGAACAAGTGAAAGGCCTT
    TACCTTTATGGTCCATTTGGGACAGGTAAATCTTTTATTCTAGGTGCAATTGCGAATCAG
    CTCAAATCTAAGAAGGTACGTTCGACAATTATTTATTTACCGGAATTTATTAGAACATTA
    AAAGGTGGCTTTAAAGATGGTTCTTTTGAAAAGAAATTACATCGCGTAAGAGAAGCAAAC
    ATTTTAATGCTTGATGATATTGGGGCTGAAGAAGTGACTCCATGGGTGAGAGATGAGGTA
    ATTGGACCTTTGCTACATTATCGAATGGTTCATGAATTACCAACATTCTTTAGTTCTAAT
    TTTGACTATAGTGAATTGGAACATCATTTAGCGATGACTCGTGATGGTGAAGAGAAGACT
    AAAGCAGCACGTATTATTGAACGTGTCAAATCTTTGTCAACACCATACTTTTTATCAGGA
    GAAAATTTCAGAAACAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

Protein[edit source | edit]

Protein Data Bank: 5HE8
Protein Data Bank: 5HE9

General[edit source | edit]

  • locus tag: SAOUHSC_01791
  • symbol: SAOUHSC_01791
  • description: primosomal protein DnaI
  • length: 306
  • theoretical pI: 7.15118
  • theoretical MW: 35635.5
  • GRAVY: -0.589543

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairchromosomal replication initiator protein DnaA (TIGR00362; HMM-score: 38.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDnaA regulatory inactivator Hda (TIGR03420; HMM-score: 21)
    AAA family ATPase, CDC48 subfamily (TIGR01243; HMM-score: 17.9)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesproteasome ATPase (TIGR03689; EC 3.6.4.8; HMM-score: 17.3)
    26S proteasome subunit P45 family (TIGR01242; HMM-score: 15.5)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairorc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 13.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairHolliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 11.5)
  • TheSEED:  
    DNA MetabolismDNA replicationDNA-replication Helicase loader DnaI 
  • PFAM:
    no clan definedDnaI_N; Primosomal protein DnaI N-terminus (PF07319; HMM-score: 94.4)
    P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 36.8)
    Bac_DnaA; Bacterial dnaA protein (PF00308; HMM-score: 31.3)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 24.5)
    AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 21.6)
    PIF1; PIF1-like helicase (PF05970; HMM-score: 18.6)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 17)
    PhoH; PhoH-like protein (PF02562; HMM-score: 16.2)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.3)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 13.8)
    KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 13.3)
    AAA_22; AAA domain (PF13401; HMM-score: 13.2)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 12.3)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.2)
    AAA_30; AAA domain (PF13604; HMM-score: 12.1)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 11.9)
    T3SS-hook (CL0424) Flg_hook; Flagellar hook-length control protein FliK (PF02120; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.128
    • Ymax_pos: 18
    • Cmax: 0.107
    • Cmax_pos: 18
    • Smax: 0.201
    • Smax_pos: 11
    • Smean: 0.141
    • D: 0.133
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTNAMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIKYDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGLYLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVREANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDGEEKTKAARIIERVKSLSTPYFLSGENFRNN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Ilya Borovok, Batia Gorovitz, Michaela Yanku, Rachel Schreiber, Bertolf Gust, Keith Chater, Yair Aharonowitz, Gerald Cohen
Alternative oxygen-dependent and oxygen-independent ribonucleotide reductases in Streptomyces: cross-regulation and physiological role in response to oxygen limitation.
Mol. Microbiol.: 2004, 54(4);1022-35
[PubMed:15522084] [WorldCat.org] [DOI] (P p)
Yan Li, Kenji Kurokawa, Luzia Reutimann, Hikaru Mizumura, Miki Matsuo, Kazuhisa Sekimizu
DnaB and DnaI temperature-sensitive mutants of Staphylococcus aureus: evidence for involvement of DnaB and DnaI in synchrony regulation of chromosome replication.
Microbiology (Reading, Engl.): 2007, 153(Pt 10);3370-9
[PubMed:17906136] [WorldCat.org] [DOI] (P p)