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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01792
  • pan locus tag?: SAUPAN004307000
  • symbol: SAOUHSC_01792
  • pan gene symbol?: dnaB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01792
  • symbol: SAOUHSC_01792
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1689026..1690426
  • length: 1401
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGACGACAAGCCTTCGAATTCGGCTTAAGACCAAAGGATCAATTCAAAGTTATGCAA
    CACTTTGATTTAAATACGAATCATTTAGAGGTATTGAATCGACTGTATACACCGCTAATT
    GGAACGCAAGCAGTGGGTTTATATCATTTTATGACTCAATTCGTTAAAGAAAGTCATAAT
    GAAACATTGATTCTTTCTCATTATATTTTTATGAATGAACTTAAAATTAATTTACTAGAG
    TTTAGACAACAAATGGACTTACTTGAAGCGATTGGATTGTTAAAGGCGTTTGTCAAACAT
    GATGAACAAGAAACACAATTTGTTTACCAATTAATTCAACCGCCTTCAGCACATTTATTT
    TTTAATGATCCAATGTTGTCAATCTTTTTATATAGTGAAGTCGAGCATCGTCGATTTCAT
    GAGTTGAAGAAATACTTTGAGTATCAACAAATTGATTTGAGTGAATTTAAACAGGTAACA
    AGACAATTTACTGATGTTTTTAAAGTGCCGTCTACAAAAATAGATATCGATACAAGTGAT
    ATTCCGATTAATGAACCGTACCAAGGTATCGATTTATCAAATGAATCTTTTGACTTTGAA
    ATGTTGAGACAAATGTTAGGTAAACATTTTATTAGTCAAGACATTGTTACCAAAGATGCA
    AAGCGTTTAATTACACAACTCGCGACTTTATATGGTTTAACAGCAGATGGCATGAAGCAT
    GTTATTTTAAATTCAATTACAAGTGGACAACAGTTATCCTTTGAAGAAATGCGTAAACAA
    GCACGCTCGTATTACTTAATGGAACACGAAAATCAAATGCCTAAATTACAGGTGAAATCT
    CCTGCTACATCATCTTCGGCGGGCAAATCTTCAGAAGTGAATCCAAAGCCACAAAGTGAT
    GAATGGTTTGAGTTACTTGAACAAACGAGTCCAATTGATATGTTAGCTTCTTGGTCTGAA
    TCAGAACCAACTATATCGCAAAAGACAATGGTTGAAGAATTAATTGAACGTGAAAAAATG
    TCTTTTGGCGTCATTAATATTTTGTTGCAATTTGTCATGTTAAAAGAAGATATGAAACTT
    CCTAAAGCGTATATTTTGGAAATAGCATCAAATTGGAAGAAAAAAGGTATTAAGACAGCT
    AAAGAAGCATATAATTACGCTAAAAAAGTTAATCAACCTAAAAATGAGGGTTCTAGCGGA
    AATTACCAAAAGCGTGGTTCATACTATGGGCAGCGTAATCGTATTTCAAAAGAAAAAACA
    CCTAAATGGTTAGAAAATAGAGATAAACCTAGTGAAGAAGATTCGGCTAAAGATAATAGC
    GTAGATGATCAACAATTAGAGCAAGATCGACAAGCATTTCTAGATAAATTATCTAAAAAA
    TGGGAGGAGGACAGTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01792
  • symbol: SAOUHSC_01792
  • description: hypothetical protein
  • length: 466
  • theoretical pI: 6.12276
  • theoretical MW: 54532.6
  • GRAVY: -0.661159

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDnaD domain protein (TIGR01446; HMM-score: 20.3)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsDnaD domain protein (TIGR01446; HMM-score: 20.3)
  • TheSEED:  
    Helicase loader DnaB 
  • PFAM:
    no clan definedDnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 39.2)
    DUF4144; protein structure with unknown function (PF13642; HMM-score: 14.3)
    SUIM_assoc; Unstructured region C-term to UIM in Ataxin3 (PF16619; HMM-score: 14.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.096
    • Ymax_pos: 25
    • Cmax: 0.111
    • Cmax_pos: 58
    • Smax: 0.151
    • Smax_pos: 2
    • Smean: 0.089
    • D: 0.093
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVTRQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSAGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Ilya Borovok, Batia Gorovitz, Michaela Yanku, Rachel Schreiber, Bertolf Gust, Keith Chater, Yair Aharonowitz, Gerald Cohen
Alternative oxygen-dependent and oxygen-independent ribonucleotide reductases in Streptomyces: cross-regulation and physiological role in response to oxygen limitation.
Mol. Microbiol.: 2004, 54(4);1022-35
[PubMed:15522084] [WorldCat.org] [DOI] (P p)