From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01793
  • pan locus tag?: SAUPAN004308000
  • symbol: nrdR
  • pan gene symbol?: nrdR
  • synonym:
  • product: transcriptional regulator NrdR

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01793
  • symbol: nrdR
  • product: transcriptional regulator NrdR
  • replicon: chromosome
  • strand: -
  • coordinates: 1690427..1690897
  • length: 471
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAATGCCCGAAATGTAATTCTACACAATCTAAAGTTGTAGATTCAAGGCATGCCGAT
    GAATTAAATGCCATTCGAAGACGAAGAGAATGTGAAAATTGTGGAACACGTTTCACTACA
    TTTGAACATATCGAAGTTAGTCAGCTTATAGTTGTGAAAAAAGATGGCACAAGAGAGCAG
    TTTTCAAGAGAAAAGATACTTAATGGACTTGTGCGTTCTTGTGAGAAACGACCAGTTAGA
    TATCAACAACTTGAAGACATAACTAACAAGGTTGAATGGCAATTACGAGATGAAGGTCAT
    ACGGAAGTGTCTTCACGAGATATAGGTGAACACGTTATGAACTTGTTAATGCATGTTGAT
    CAAGTTTCATATGTTAGATTTGCATCAGTCTATAAAGAATTTAAAGATGTTGATCAATTG
    TTAGCATCGATGCAAGGGATTTTAAGCGAAAACAAACGGAGTGATGCTTAA
    60
    120
    180
    240
    300
    360
    420
    471

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01793
  • symbol: NrdR
  • description: transcriptional regulator NrdR
  • length: 156
  • theoretical pI: 7.89688
  • theoretical MW: 18203.5
  • GRAVY: -0.744872

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionstranscriptional regulator NrdR (TIGR00244; HMM-score: 187.7)
    Cys-rich peptide, TIGR04165 family (TIGR04165; HMM-score: 16.4)
    Signal transductionRegulatory functionsDNA interactionsputative regulatory protein, FmdB family (TIGR02605; HMM-score: 14.6)
    MJ0042 family finger-like domain (TIGR02098; HMM-score: 12)
    cxxc_20_cxxc protein (TIGR04104; HMM-score: 8.7)
  • TheSEED:  
    Nucleosides and NucleotidesNucleosides and Nucleotides - no subcategoryRibonucleotide reduction Ribonucleotide reductase transcriptional regulator NrdR 
  • PFAM:
    no clan definedATP-cone; ATP cone domain (PF03477; HMM-score: 65.1)
    C2H2-zf (CL0361) zf-DBF; DBF zinc finger (PF07535; HMM-score: 19.1)
    Zn_Beta_Ribbon (CL0167) TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 16.3)
    Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 15.4)
    Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 14.6)
    zinc_ribbon_5; zinc-ribbon domain (PF13719; HMM-score: 14.1)
    zf-FYVE-PHD (CL0390) FYVE; FYVE zinc finger (PF01363; HMM-score: 14)
    Zn_Beta_Ribbon (CL0167) Elf1; Transcription elongation factor Elf1 like (PF05129; HMM-score: 13.8)
    TFIIS_C; Transcription factor S-II (TFIIS) (PF01096; HMM-score: 12.2)
    C2H2-zf (CL0361) zf-C2H2; Zinc finger, C2H2 type (PF00096; HMM-score: 11.1)
    Zn_Beta_Ribbon (CL0167) zinc_ribbon_2; zinc-ribbon domain (PF13240; HMM-score: 8.7)
    zinc_ribbon_4; zinc-ribbon domain (PF13717; HMM-score: 8.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.181
    • Ymax_pos: 20
    • Cmax: 0.14
    • Cmax_pos: 20
    • Smax: 0.439
    • Smax_pos: 1
    • Smean: 0.257
    • D: 0.211
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHIEVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTEVSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Ilya Borovok, Batia Gorovitz, Michaela Yanku, Rachel Schreiber, Bertolf Gust, Keith Chater, Yair Aharonowitz, Gerald Cohen
Alternative oxygen-dependent and oxygen-independent ribonucleotide reductases in Streptomyces: cross-regulation and physiological role in response to oxygen limitation.
Mol. Microbiol.: 2004, 54(4);1022-35
[PubMed:15522084] [WorldCat.org] [DOI] (P p)